Case study report on sugarcanePresentation Transcript
Case study report on Sugarcane Submitted by : Arun N ID Number: 008078 Module: D2D001 School of Bioscience, Nottingham University Recent advances in research on plant tissue culture of Sugarcane 1
Sugarcane is one of the most important crops in the world. Brazil is the largest sugarcane grower, they grow it for: Sugar – Production of food & alcoholic beverages Ethanol – Gasoline + ethanol blend as fuel In sugarcane, micropropagation is important for rapid multiplication of elite genotypes/clones and for the spreading of new varieties. Research attention on tissue culture of sugarcane was intensified due to its economic importance as a cash crop. 2 Sugarcane tissue culture
In vitro plant regeneration using shoot tip culture. In vitro micropropagation through callus culture. Somaclonal variation – screening for salt tolerance. 3 Recent research study on
Explants used are: Young leaves Shoot tips Meristem cells Leaf sheath 4 Laboratory details Common surface sterilization techniques are followed: Steps highlighted in green color are carried out inside the flow chamber
In vitro micropropagation of Sugarcane through callus culture
For best callus induction - medium is supplemented with 10% coconut water.
In vitro shoots are inoculated on half-strength MS medium for better rooting.
2,4-D conc. 2.5-3.0mg/L is best for callus induction.
NAA 3.0mg/L along with ½ strength MS medium is good for rooting.
In vitro plant regeneration using shoot tip culture 6 For shoot regeneration, cytokinin BAP was more effective than Kn and IBA. Auxin is essential for root initiation. NAA is best for rooting. Whereas, IAA or IBA produce poor quality roots. Rate of multiplication is high in adventitious shoots.
Plants regenerated from meristem (shoot tip) are very similar, both Phenotypically Genotypically, to the parent plant. Shoot tip culture is better than leaf roll culture for plant production. For shoot regeneration the combination of auxin and cytokinin is essential. Regeneration potential of callus was specific and genotype dependent and also parallel with hormonal conc. and combination. 7 In vitro propagation of Sugarcane
8 In vitro callus regeneration and plant establishment (1) Callus regeneration in MS+2.5mg/l 2,4-D (2) Callus regeneration in MS+2.5mg/l NAA (3&4) Multiple shoot emergence from callus tissue in MS+2.0 mg/l BAP+0.5mg/l IBA (5) Micro shoots rooted in 1/2MS+NAA(2.5 mg/l). (6) Hardening of rooted plant lets in plastic trays.
Somaclonal variation The term somaclonal variation was first introduced by Scow Croft and Larkin (1981) In Saccharum Sp somaclonal variation is termed as sub-clonal variation. Salt tolerant plants were produced by tissue culture techniques Khan et al., (2004) Explant – young leaf Regeneration medium – Fe-EDTA 1mg/L, Casein Hydrolyzate 500mg/L, Cystine free base 30mg/L Salt tolerant soma-clones performed better in the above mentioned characteristics 9
Over the past 10 years tissue culture technology of sugarcane has been developed, but not without some challenges. Work on Tissue culture should integrate hand in hand with molecular techniques, it will be helpful in obtaining genetically stable plants. Mass propagation of Sugarcane through Tissue culture approach will result in availability of disease free germplasm, propagation of newer germplasm with improved agronomic characters for the ultimate benefit of the farmers. Sugarcane tissue culture work can be directed towards germplasm conservation also. 10 Conclusion
In order to ascertain the true to type character of the Tissue culture raised plantlets through shoot proliferation, it is essential to check at the genomic level, it is felt that further experimentation is needed towards achieving the result. 11 Further studies
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