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Article Reference Number: OPP 029
In-vitro and In-vivo anti-inflammatory potential of Tabernaemontana divaricata leaves
Sumithra Mohan*, J. Anbu, Arijit Chakraborty, Ariadasy Canagasaby, Odelia Sawian
School of Pharmaceutical Sciences, Vels University, VISTAS
Pallavaram, Chennai -117.
• Inflammation is a non specific, localized immune reaction of the
organism, which tries to localized the pathogenic agents. Many
consider the syndrome a self- defense mechanism.
• It consist in vascular, metabolic, cellular changes, triggered by the
entering of pathogenic agents in healthy tissues of the body.
Mechanism action of Anti-inflammatory drugs.
inflammation and fever
Ta be rna e m o nta na d iva ric a ta (Family - Apocynaceae) is a
beautiful evergreen shrub, about 54cm high, with large shiny
leaves, crepe jasmine flowers, may appear sporadically all year. It
, is a rich source of alkaloids with various pharmacological
T. d iva ric a ta
Ref: Kar B; Suresh Kumar RB; Karmakar I; Dolai N, Bala A;
Mazumder UK, Haldar PK; Antioxidant and in vitro antiinflammatory activities of Mimusops elengi leaves; Asian
Pacific Journal of Tropical Biomedicine; Vol. 2012; pp S976S980
The percentage of HRBC membrane stabilization or protection was
calculated using the formula:
O.D of Test Solution – O.D
of Product Control
Percentage Inhibition of Haemolysis = 100 –
O.D of Test
Observation table for In-vitro study
% Prevention of Lysis
Effect of concentration on %
prevention of lysis of extract treated
Effect of concentration on % inhibition
of lysis of diclofenac treated group
Ref: Winter CA; Risley EA; Nuss
GW; Carrageenin induced
oedema in hind paw of rat as an
assay for anti-inflammatory drugs;
Proc Soc Exp Biol Med 1962; 111;
EETD*: Ethanolic extract of
Ta be rna e m o nta na d iva ric a ta
Observation table for In-vivo study
0.325± Ta be
EETD*: 200 mg/kg Extract of0.081 rna e m o nta na
d iva ric a ta .
Effects of dose on mean increase in
Effect of dose on percentage inhibition
Result for In-vitro study (Table 1) :
The extract at concentration range of 6.3 -100 mg/ml protect
the human erythrocyte membrane against lysis induced by
At concentration of 100µg/ml, the extract produced 47.36 %
inhibition of RBC haemolysis as compared with 48.14% produced
by diclofenac sodium.
Result for In-vivo study (Table 2) :
The extract at concentration 200 mg/kg and 400 mg/kg protect
the human erythrocyte membrane against carrageen induced.
At concentration 200 mg/kg and 400 mg/kg, the extract produce
36.02 % and 42.63 % inhibition of inflammation as compared with
58.13 % produced by diclofenac sodium.
In conclusion the present study demonstrated that
Ta be rna e m o nta na d iv a ric a ta leaves extract has anti-inflammatory
activity at a dose of 100 µg/ml in in-vitro study and 200 mg/kg and
400 mg/kg in in-vivo study.
The authors are thankful to the authority of the School Of
Pharmaceutical Science, Vels University, Pallavaram, Chennai 600117, India, for providing necessary facilities for the present study.