Experimental Techniques

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Slides on experimental methods for Developmental Biology, spring 2012

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Experimental Techniques

  1. 1. ExperimentalTechniques
  2. 2. Chapters 3 and 5
  3. 3. Chapter 10
  4. 4. Gene Cloning
  5. 5. Figure 10-13 Essential Cell Biology (© Garland Science 2010)
  6. 6. Figure 10-14 Essential Cell Biology (© Garland Science 2010)
  7. 7. Polymerase Chain Reaction(PCR)
  8. 8. Figure 10-15 Essential Cell Biology (© Garland Science 2010)
  9. 9. Figure 10-16 Essential Cell Biology (© Garland Science 2010)
  10. 10. Figure 10-17 Essential Cell Biology (© Garland Science 2010)
  11. 11. Mutagenesis
  12. 12. Figure 10-34 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)
  13. 13. Figure 10-34 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)
  14. 14. Gene Knock-Out
  15. 15. Figure 10-35 Essential Cell Biology (© Garland Science 2010)
  16. 16. Gene Replacement
  17. 17. Figure 10-35 Essential Cell Biology (© Garland Science 2010)
  18. 18. Reporter Genes
  19. 19. Figure 10-29a Essential Cell Biology (© Garland Science 2010)
  20. 20. Figure 10-30 Essential Cell Biology (© Garland Science 2010)
  21. 21. Transgenesis
  22. 22. Figure 10-36 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)
  23. 23. Figure 10-36 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)
  24. 24. Figure 10-37 Essential Cell Biology (© Garland Science 2010)
  25. 25. RNAi
  26. 26. microRNA (miRNA) Short, noncoding, regulatoryRNAs controlling gene expression
  27. 27. Figure 8-26 Essential Cell Biology (© Garland Science 2010)
  28. 28. small interfering RNA (siRNA) Short, double-strandedRNAs controlling gene expression
  29. 29. Figure 8-27 Essential Cell Biology (© Garland Science 2010)
  30. 30. Figure 10-38a Essential Cell Biology (© Garland Science 2010)
  31. 31. Figure 10-38b,c Essential Cell Biology (© Garland Science 2010)
  32. 32. Nucleic AcidHybridization
  33. 33. Figure 10-31 Essential Cell Biology (© Garland Science 2010)
  34. 34. DNA Microarrays
  35. 35. Figure 10-33 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)
  36. 36. Figure 10-33 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)
  37. 37. Blotting
  38. 38. Figure 10-5 Essential Cell Biology (© Garland Science 2010)
  39. 39. Figure 10-5 (part 1 of 5) Essential Cell Biology (© Garland Science 2010)
  40. 40. Gel Electrophoresis
  41. 41. Figure 10-3 Essential Cell Biology (© Garland Science 2010)
  42. 42. Figure 10-5 (part 2 of 5) Essential Cell Biology (© Garland Science 2010)
  43. 43. Figure 10-5 (part 3 of 5) Essential Cell Biology (© Garland Science 2010)
  44. 44. Figure 10-5 (part 4 of 5) Essential Cell Biology (© Garland Science 2010)
  45. 45. Figure 10-5 (part 5 of 5) Essential Cell Biology (© Garland Science 2010)
  46. 46. Figure 10-5 Essential Cell Biology (© Garland Science 2010)
  47. 47. Immunostaining
  48. 48. Fusion Proteins

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