The Academic Center and Development of Future Treatments for Malignant Gliomas <ul><li>Thomas C. Chen, MD, PhD </li></ul><...
Malignant Gliomas <ul><li>18,000 new cases of primary CNS tumors in the US </li></ul><ul><li>At least 10x that number for ...
Typical Treatment Course for Gliomas 1.  Surgery-biopsy or resection 2.  Radiation with low dose temozolomide (TMZ) 3.  Hi...
 
 
 
Preop Axial T1  with Contrast
Pre-resection motor stimulation with Ojemann Stimulator
Post Resection:  Motor Stimulation
Gliadel Wafers Placed
Glioblastoma:  post-mortem
Goal of Talk <ul><li>1.  New chemotherapy paradigms </li></ul><ul><li>a.  ER stress-DMC, Nelfinavir, EGCG </li></ul><ul><l...
 
Glioma Research Group <ul><li>Thomas Chen, MD,PhD-Neurosurgery, Pathology </li></ul><ul><li>Florence Hofman, PhD-Pathology...
Targeting the Perinecrotic Rim in Glioblastoma Multiforme via Modulation of the Endoplasmic Reticulum Stress Response Vasc...
Targeting the Perinecrotic Rim in Glioblastoma Multiforme via  Modulation of the Endoplasmic Reticulum Stress Response
Concept of ER stress <ul><li>1.  Glioma cells are “stressed” because of local microenvironment, and external stimuli from ...
<ul><li>DMC and Nelfinavir-Increase ER stress </li></ul>
Targeting the Perinecrotic Rim in Glioblastoma Multiforme via  Modulation of the Endoplasmic Reticulum Stress Response
•
HIV-1 Protease Inhibitors Nelfinavir and Atazanavir Induce Malignant Glioma Death by Triggering Endoplasmic Reticulum Stress
GRP78 LEVELS IN GLIOBLASTOMA TISSUE SECTIONS
TMZ increases GRP78 in glioblastoma cell lines. GRP78 Actin GRP78 Actin
U87 glioblastoma cells transfected with different concentrations of GRP78 siRNA  and GFP siRNA fused to rhodamine.
 
 
 
Glioma Cancer Stem Cells and ER Stress
Neural Stem Cells and the  Origin of Gliomas Nader Sanai, M.D., Arturo Alvarez-Buylla, Ph.D., and Mitchel S. Berger, M.D.
Glioma Cancer Stem Cells (GCSC) <ul><li>What do we know? </li></ul><ul><li>1)  Progenitor cells for development of GBM </l...
x16 x48 Morphology of GCSC (D431)
TMZ treatment with 15% BSA Treatment in 6well plate and transfer to 96well plate for MTT analysis  2009-10-01 TMZ (uM) % O...
Is DMC Cytotoxic To Glioma Cancer Stem Cells (GCSC) ?  U87 U251 CSC (single) CSC (sphere) DMC (uM) % O.D. at 490nm Conditi...
Does DMC Enduces ER Stress in Glioma Cancer Stem Cells (CSC) ?  1  2  3  4  5  GRP78 Chop Actin 1: No treatment 2: DMC 20u...
Does DMC Induce ER Stress in Glioma Cancer Stem Cells (CSC) ?  1  2  3  4  5  GRP78 Chop Actin Parp MGMT Conclusion: ER st...
 
 
Perillyl Alcohol (POH) <ul><li>1.  POH is a natural distillate from plants (pines). </li></ul><ul><li>2.  It is sold comme...
 
May/2006 Dec/2006 Fibrillary Astrocytoma grade II ________________________________________________________________________...
JAN / 2007 MAY / 2008 Recurrente Anaplastic Oligodendroglioma ____________________________________________________________...
Disease-free survival (6 months) _________________________________________________________________________________________...
USA DEVELOPMENT <ul><li>1.  Synthesis of pharmaceutical grade perillyl alcohol (Norac Pharmaceuticals).  Our synthetic POH...
 
 
Development of an Implantable Pump for Convection Enhanced Delivery
What’s currently available for drug delivery for brain tumors <ul><li>1.  Oral Chemotherapy </li></ul><ul><li>2.  Intraven...
CONVECTION ENHANCED DELIVERY <ul><li>Direct delivery of drug into tumor </li></ul><ul><li>Drug delivered for 4-5 days via ...
 
www.pharmaco-kinesis.com Convection Enhanced Trials 1.  Some success in Phase I/II trials  2.  No clear success in Phase I...
<ul><li>Photograph depicting the different types of catheters tested in the gel experiments. Scale on the left side of the...
<ul><li>Screenshot of the iPlan! flow application (version 2) showing the planned trajectories for five catheters and the ...
 
<ul><li>What if?  We had a novel delivery device that could be: </li></ul><ul><ul><li>implanted-medication delivered conti...
9/19/2008 Property of Pharmaco Kinesis Corp.
<ul><li>The catheter will serve as a means of delivery as well as analysis on the environment in the brain. </li></ul><ul>...
Fig. 8 www.pharmaco-kinesis.com Medication Storage and Delivery System Circulation pump Medication pump Medication Reservo...
 
www.pharmaco-kinesis.com
www.pharmaco-kinesis.com
Fig. 3B www.pharmaco-kinesis.com
<ul><li>Bi-directional communication. </li></ul><ul><li>Low power consumption “wake-up” circuitry. </li></ul><ul><li>One c...
Cir. And Reservoir Pump ( Bartels ) Active Valve ( Bartels ) Flow Sensor ( Sensirion ) Pressure Sensor Temperature Sensor ...
www.pharmaco-kinesis.com Wireless Radio High level goals High level tasks Internal states Growth signal Intracranial press...
What type of drugs to place? <ul><li>1.  Avastin </li></ul><ul><li>2.  Irinotecan </li></ul><ul><li>3.  Topotecan </li></u...
www.pharmaco-kinesis.com Avastin in 28-day pump Implantated IP CPT-11 D7 – D11 D14 – D118 D21 – D25 D28 – D32 1 st  dose o...
VIDEO OF IMPLANTABLE PUMP <ul><li>1.  Key attributes </li></ul><ul><li>a.  Implantable </li></ul><ul><li>b.  Physiological...
 
RCR vectors for  intracellular chemotherapy   <ul><li>CD converts the non-toxic prodrug 5-FC to the toxic metabolite 5-FU ...
U87 intra-cerebral glioma model for  in vivo  analysis of RCR vector-mediated suicide gene therapy Establishment  of tumor...
RCR-CD + 5-FC achieves significantly prolonged survival in an intracranial glioma model <ul><li>Doubling of survival time ...
RCR-mediated suicide gene therapy achieves significant inhibition of primary intracranial tumor growth <ul><li>Anti-MLV im...
U01:  Pre-clinical Development of RCR  <ul><li>1.  Multi-center NIH grant:  UCLA, USC, UCSF </li></ul><ul><li>2.  Develop ...
Phase I/II Clinical Trial <ul><li>1.  Tocagen, Inc. raised money and spearheading trial </li></ul><ul><li>2.  Treatment Si...
Immunotherapy <ul><li>1.  Immune therapy based on recognition by immune system of tumor antigens vs self antigens. </li></...
Immunotherapy <ul><li>1.  Development of new vaccine-based on combination of allogeneic and syngeneic tumor vaccine </li><...
CONCLUSION <ul><li>1.  New treatment paradigms in malignant glioma therapy based on translational research. </li></ul><ul>...
Future Development <ul><li>1.  Miniaturization Started </li></ul><ul><li>2.  In-vitro agarose brain models </li></ul><ul><...
CONCLUSION <ul><li>1.  Brain tumor pump-resectable and unresectable tumors, leptomeningeal carcinomatosis.  Goal is to imp...
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Dr. Thomas Chen, UCI grand rounds 7-28-2010

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Dr. Thomas Chen, UCI grand rounds 7-28-2010

  1. 1. The Academic Center and Development of Future Treatments for Malignant Gliomas <ul><li>Thomas C. Chen, MD, PhD </li></ul><ul><li>Associate Professor, Neurosurgery and Pathology </li></ul><ul><li>Director, Surgical Neuro-oncology, USC </li></ul>
  2. 2. Malignant Gliomas <ul><li>18,000 new cases of primary CNS tumors in the US </li></ul><ul><li>At least 10x that number for metastatic brain tumors </li></ul><ul><li>Most common type of primary CNS tumor-malignant glioma </li></ul><ul><li>Currently no cure-despite improvements in surgery, chemotherapy, radiation therapy </li></ul><ul><li>Grades I-IV: Worst grade-glioblastoma multiforme-survival of 12-15 months </li></ul>
  3. 3. Typical Treatment Course for Gliomas 1. Surgery-biopsy or resection 2. Radiation with low dose temozolomide (TMZ) 3. High dose or metronomic TMZ alone 4. F/u with MRI scans every two months 5. If MRI documented progression: Avastin, repeat resection, radiosurgery, or clinical trials
  4. 7. Preop Axial T1 with Contrast
  5. 8. Pre-resection motor stimulation with Ojemann Stimulator
  6. 9. Post Resection: Motor Stimulation
  7. 10. Gliadel Wafers Placed
  8. 11. Glioblastoma: post-mortem
  9. 12. Goal of Talk <ul><li>1. New chemotherapy paradigms </li></ul><ul><li>a. ER stress-DMC, Nelfinavir, EGCG </li></ul><ul><li>b. ER stress and cancer stem cells </li></ul><ul><li>c. New concepts of glioma vasculature and </li></ul><ul><li>anti-angiogenesis treatments </li></ul><ul><li>2. New modes of delivery </li></ul><ul><li>a. Intranasal delivery of chemotherapy </li></ul><ul><li>b. Brain tumor pump </li></ul><ul><li>3. New approach to gene therapy-replication competent </li></ul><ul><li>retrovirus </li></ul><ul><li>4. Conceptual switch in immunotherapy </li></ul>
  10. 14. Glioma Research Group <ul><li>Thomas Chen, MD,PhD-Neurosurgery, Pathology </li></ul><ul><li>Florence Hofman, PhD-Pathology </li></ul><ul><li>Stan Louie, PharmD, PhD-Pharmacy </li></ul><ul><li>Nicos Petasis, PhD-Chemistry </li></ul><ul><li>Axel Schonthal, PhD-Microbiology </li></ul>
  11. 15. Targeting the Perinecrotic Rim in Glioblastoma Multiforme via Modulation of the Endoplasmic Reticulum Stress Response Vascularized Periphery Perinecrotic Rim (Pseudopalisades ) Necrotic Area Thrombotic Vessel
  12. 16. Targeting the Perinecrotic Rim in Glioblastoma Multiforme via Modulation of the Endoplasmic Reticulum Stress Response
  13. 17. Concept of ER stress <ul><li>1. Glioma cells are “stressed” because of local microenvironment, and external stimuli from radiation, chemotherapy </li></ul><ul><li>2. Use drugs to induce greater “stress” and knock cells into apoptosis </li></ul><ul><li>3. Use cells to decrease glioma cell resistance ie GRP78, and make them more chemosensitive. </li></ul>
  14. 18. <ul><li>DMC and Nelfinavir-Increase ER stress </li></ul>
  15. 19. Targeting the Perinecrotic Rim in Glioblastoma Multiforme via Modulation of the Endoplasmic Reticulum Stress Response
  16. 20.
  17. 21. HIV-1 Protease Inhibitors Nelfinavir and Atazanavir Induce Malignant Glioma Death by Triggering Endoplasmic Reticulum Stress
  18. 22. GRP78 LEVELS IN GLIOBLASTOMA TISSUE SECTIONS
  19. 23. TMZ increases GRP78 in glioblastoma cell lines. GRP78 Actin GRP78 Actin
  20. 24. U87 glioblastoma cells transfected with different concentrations of GRP78 siRNA and GFP siRNA fused to rhodamine.
  21. 28. Glioma Cancer Stem Cells and ER Stress
  22. 29. Neural Stem Cells and the Origin of Gliomas Nader Sanai, M.D., Arturo Alvarez-Buylla, Ph.D., and Mitchel S. Berger, M.D.
  23. 30. Glioma Cancer Stem Cells (GCSC) <ul><li>What do we know? </li></ul><ul><li>1) Progenitor cells for development of GBM </li></ul><ul><li>2) Present in gliomas-constitute 1-2% of tumor population </li></ul><ul><li>3) Radiation and chemotherapy resistant </li></ul><ul><li>4) Very invasive </li></ul><ul><li>5) Hypothesized to be one of the main reasons for recurrence </li></ul><ul><li>6) Different markers-including CD133, CD15 </li></ul><ul><li>7) Hypothesis: ER stress may be used to treat glioma cancer stem cells </li></ul>
  24. 31. x16 x48 Morphology of GCSC (D431)
  25. 32. TMZ treatment with 15% BSA Treatment in 6well plate and transfer to 96well plate for MTT analysis 2009-10-01 TMZ (uM) % O.D. at 490nm TMZ (uM) % O.D. at 490nm Conclusion: TMZ is not cytotoxic to CSC. U251 w/ 15% BSA CSC w/ 15% BSA 0 20 40 60 80 100 120 0 10 20 30 40 50 60 70 80 90 100 0 20 40 60 80 100 120 0 10 20 30 40 50 60 70 80
  26. 33. Is DMC Cytotoxic To Glioma Cancer Stem Cells (GCSC) ? U87 U251 CSC (single) CSC (sphere) DMC (uM) % O.D. at 490nm Condition: Medium +10% BSA, 48hr treatment 0 20 40 60 80 100 120 140 0 10 20 30 40 50 60 70 80 90 100
  27. 34. Does DMC Enduces ER Stress in Glioma Cancer Stem Cells (CSC) ? 1 2 3 4 5 GRP78 Chop Actin 1: No treatment 2: DMC 20uM 3: DMC 40uM 4: DMC 60uM 5: DMC 80uM
  28. 35. Does DMC Induce ER Stress in Glioma Cancer Stem Cells (CSC) ? 1 2 3 4 5 GRP78 Chop Actin Parp MGMT Conclusion: ER stress upregulated at DMC 60 uM in CSC. DMC at 60 uM reduces MGMT levels. 24hr treatment Cl. parp Parp 1: No treatment 2: DMC 20uM 3: DMC 40uM 4: DMC 60uM 5: DMC 80uM CSC-D431
  29. 38. Perillyl Alcohol (POH) <ul><li>1. POH is a natural distillate from plants (pines). </li></ul><ul><li>2. It is sold commercially for laboratory purposes </li></ul><ul><li>3. It has been used as oral chemotherapy in Phase I trials in the US for systemic cancer </li></ul><ul><li>4. Overall toxicity was mild, with the most severe being gastrointestinal intolerance </li></ul><ul><li>5. Clovis Fonseca Brazilian neurosurgeon-used POH for nasal inhalation in the treatment of malignant gliomas. To date, 140 patients have been treated. </li></ul>
  30. 40. May/2006 Dec/2006 Fibrillary Astrocytoma grade II ______________________________________________________________________________________________________________________________________ May/2008 Jun/2007
  31. 41. JAN / 2007 MAY / 2008 Recurrente Anaplastic Oligodendroglioma ______________________________________________________________________________________________________________________________________ NOV / 2008
  32. 42. Disease-free survival (6 months) ______________________________________________________________________________________________________________________________________ The median PFS rate was 48.2% for patients with GBM, 60% for patients with AA, and 66.6% for patients with AO.
  33. 43. USA DEVELOPMENT <ul><li>1. Synthesis of pharmaceutical grade perillyl alcohol (Norac Pharmaceuticals). Our synthetic POH is more potent than the commercially available one. </li></ul><ul><li>2. We will be performing toxicity studies and obtaining IND from FDA to run a Phase I clinical trial for our synthetic POH in patients with recurrent GBM. </li></ul><ul><li>3. IP in development: a) use of POH as a solvent to dissolve standard chemotherapy for inhalational therapy b) chemically link POH with chemotherapeutic agents to make new drug </li></ul>
  34. 46. Development of an Implantable Pump for Convection Enhanced Delivery
  35. 47. What’s currently available for drug delivery for brain tumors <ul><li>1. Oral Chemotherapy </li></ul><ul><li>2. Intravenous Chemotherapy </li></ul><ul><li>3. Tumor Wafers (ie Gliadel) </li></ul><ul><li>4. Convection Enhanced Delivery </li></ul>www.pharmaco-kinesis.com
  36. 48. CONVECTION ENHANCED DELIVERY <ul><li>Direct delivery of drug into tumor </li></ul><ul><li>Drug delivered for 4-5 days via external catheter which is then removed </li></ul><ul><li>If drug needed to be infused again, need another surgery for catheter implantation </li></ul>
  37. 50. www.pharmaco-kinesis.com Convection Enhanced Trials 1. Some success in Phase I/II trials 2. No clear success in Phase III trials (Neopharm, Transmid) 3. Question: Lack of success from delivery or from drug?
  38. 51. <ul><li>Photograph depicting the different types of catheters tested in the gel experiments. Scale on the left side of the image is 1 mm. </li></ul>
  39. 52. <ul><li>Screenshot of the iPlan! flow application (version 2) showing the planned trajectories for five catheters and the results of simulated infusion from these positions. </li></ul>
  40. 54. <ul><li>What if? We had a novel delivery device that could be: </li></ul><ul><ul><li>implanted-medication delivered continuously without reimplanting catheter </li></ul></ul><ul><ul><li>metronomic continuous delivery of drug </li></ul></ul><ul><ul><li>Adjustable delivery controlled externally </li></ul></ul><ul><ul><li>instant monitoring and feedback of tumor parameters-ie VEGF </li></ul></ul><ul><ul><li>minimize systemic side-effects of chemotherapy </li></ul></ul><ul><ul><li>do not have to worry about blood brain barrier </li></ul></ul><ul><ul><li>Bypass first pass hepatic metabolism </li></ul></ul>www.pharmaco-kinesis.com
  41. 55. 9/19/2008 Property of Pharmaco Kinesis Corp.
  42. 56. <ul><li>The catheter will serve as a means of delivery as well as analysis on the environment in the brain. </li></ul><ul><li>Catheter will be equipped with measuring devices utilizing optics to avoid any interference with electric, magnetic, and radio-frequency devices. </li></ul><ul><li>These devices will be capable </li></ul><ul><li>of measuring pressure, </li></ul><ul><li>temperature, pH, and levels </li></ul><ul><li>of dissolved oxygen. </li></ul>9/19/2008 Property of Pharmaco Kinesis Corp. www.pharmaco-kinesis.com
  43. 57. Fig. 8 www.pharmaco-kinesis.com Medication Storage and Delivery System Circulation pump Medication pump Medication Reservoirs Catheter Catheter Cross Section Fluid Circulation System
  44. 59. www.pharmaco-kinesis.com
  45. 60. www.pharmaco-kinesis.com
  46. 61. Fig. 3B www.pharmaco-kinesis.com
  47. 62. <ul><li>Bi-directional communication. </li></ul><ul><li>Low power consumption “wake-up” circuitry. </li></ul><ul><li>One chipset in both the device and a base station. </li></ul>9/19/2008 Property of Pharmaco-Kinesis Corp. www.pharmaco-kinesis.com
  48. 63. Cir. And Reservoir Pump ( Bartels ) Active Valve ( Bartels ) Flow Sensor ( Sensirion ) Pressure Sensor Temperature Sensor ( GE ) pH Sensor ( WPI ) Reservior ( Metal Bellows ) www.pharmaco-kinesis.com
  49. 64. www.pharmaco-kinesis.com Wireless Radio High level goals High level tasks Internal states Growth signal Intracranial pressure Tissue condition Tissue metabolism Table of parameters Circulate Inject 1 Inject 2 Inject 3 Pump Pump-valve Pump-valve Pump-valve VEGF Spectrometer Pressure sensor pH sensor Oxygen sensor Solid Tumor Microcontroller Medication Tumor Fluid
  50. 65. What type of drugs to place? <ul><li>1. Avastin </li></ul><ul><li>2. Irinotecan </li></ul><ul><li>3. Topotecan </li></ul><ul><li>4. IL-13 toxin, IL-4 toxin, Diphteria toxin </li></ul>www.pharmaco-kinesis.com
  51. 66. www.pharmaco-kinesis.com Avastin in 28-day pump Implantated IP CPT-11 D7 – D11 D14 – D118 D21 – D25 D28 – D32 1 st dose of Avastin 2nd dose of Avastin
  52. 67. VIDEO OF IMPLANTABLE PUMP <ul><li>1. Key attributes </li></ul><ul><li>a. Implantable </li></ul><ul><li>b. Physiological measurement with lab on chip to determine progress of chemotherapy </li></ul><ul><li>c. Multiple delivery bellows for different cycles of chemotherapy </li></ul><ul><li>d. Remote wi-fi control of pump </li></ul><ul><li>2. Development Stage </li></ul><ul><li>a. Pig model for implantation </li></ul><ul><li>b. Delivery into CSF for leptomeningeal model </li></ul><ul><li>3. Clinical Development Model </li></ul><ul><li>a. Leptomeningeal Disease </li></ul><ul><li>b. Solid tumors-recurrent GBM </li></ul>
  53. 69. RCR vectors for intracellular chemotherapy <ul><li>CD converts the non-toxic prodrug 5-FC to the toxic metabolite 5-FU </li></ul> gag pol env U5 R CMV U5 R U3 IRES CD 5-fluorocytosine (non-toxic) 5-fluorouracil (toxic)
  54. 70. U87 intra-cerebral glioma model for in vivo analysis of RCR vector-mediated suicide gene therapy Establishment of tumor 1 x 10 t.u. ACE-CD injected into tumor 4 Intraperitoneal injection of 5-FC H&E / IHC Survival analysis virus spread 2 x 10 U87 tumor cells injected into nude mice 5
  55. 71. RCR-CD + 5-FC achieves significantly prolonged survival in an intracranial glioma model <ul><li>Doubling of survival time in ACE-CD/5FC-treated animals </li></ul><ul><li>after only a single cycle of virus + pro-drug administration </li></ul>▪ Eventual death of treated animals due to metastatic spread of glioma cells to ectopic intracranial sites (cerebellum, brainstem) median survival: ≈ 65 days
  56. 72. RCR-mediated suicide gene therapy achieves significant inhibition of primary intracranial tumor growth <ul><li>Anti-MLV immunohistochemistry confirms viral replication </li></ul><ul><li>localized to tumor without spread to surrounding normal brain </li></ul><ul><li>No viable tumor cells left in RCR-injected primary lesion </li></ul>
  57. 73. U01: Pre-clinical Development of RCR <ul><li>1. Multi-center NIH grant: UCLA, USC, UCSF </li></ul><ul><li>2. Develop optimal conditions for GMP retroviral delivery, treatment, and detection of retroviral spread in preclinical models </li></ul>
  58. 74. Phase I/II Clinical Trial <ul><li>1. Tocagen, Inc. raised money and spearheading trial </li></ul><ul><li>2. Treatment Sites: UCSF, UCLA, Cleveland Clinic </li></ul><ul><li>3. Recruit 12 patients for RCR implantation and treatment </li></ul><ul><li>4. Radiographic demonstration of retroviral spread via MR spectroscopy-document at USC under Dr. Walter Wolf </li></ul>
  59. 75. Immunotherapy <ul><li>1. Immune therapy based on recognition by immune system of tumor antigens vs self antigens. </li></ul><ul><li>2. Tumor cells various mechanisms of immune escape-including loss of Class I MHC expression. </li></ul><ul><li>3. Immune therapy safe-but success still elusive </li></ul>
  60. 76. Immunotherapy <ul><li>1. Development of new vaccine-based on combination of allogeneic and syngeneic tumor vaccine </li></ul><ul><li>2. Allogeneic-based on establishment of brain tumor bank of different races </li></ul><ul><li>3. Southern California Immunotherapy Group: USC, UCLA, UC Irvine, City of Hope, Loma Linda </li></ul><ul><li>4. International combination with Belgium vaccine company. </li></ul>
  61. 77. CONCLUSION <ul><li>1. New treatment paradigms in malignant glioma therapy based on translational research. </li></ul><ul><li>2. Understanding of ER stress, tumor microenvironment, glioma endothelial cells. </li></ul><ul><li>3. New methods of delivery for chemotherapy including intranasal delivery and implanted brain tumor pump </li></ul><ul><li>4. More aggressive approaches to gene therapy and immunotherapy. </li></ul>
  62. 78. Future Development <ul><li>1. Miniaturization Started </li></ul><ul><li>2. In-vitro agarose brain models </li></ul><ul><li>3. In-vivo pig brain implants-non-survival/survival models </li></ul><ul><li>4. Initial development for simpler disease models: leptomeningeal carcinomatosis </li></ul>
  63. 79. CONCLUSION <ul><li>1. Brain tumor pump-resectable and unresectable tumors, leptomeningeal carcinomatosis. Goal is to improve local control, part of multi-disciplinary treatment, metronomic delivery. </li></ul><ul><li>2. Physiological feedback on treatment progress measured via lab on chip parameters. </li></ul><ul><li>3. Wi-Fi external control of multiple agents for cycle delivery. </li></ul>www.pharmaco-kinesis.com

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