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Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm
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Matrix Assisted laser desorption Ioization technique by Anubhav singh, M.pharm

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MALDI Technique of ionization

MALDI Technique of ionization

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  • 1.   Presented By- Anubhav Singh M.pharm 1st Year IPR, GLA University
  • 2.          Introduction Mechanism Instrument MALDI Matrix MALDI Laser Time Mass Detector Application Conclusion References
  • 3.  Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectroscopy, allowing the analysis of biomolecules (biopolymers such as DNA, Proteins, peptide and sugars) and organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods.  Method where a laser is used to generate ions of high molecular weight samples, such as proteins and polymers.
  • 4.    The mechanism remains uncertain. It may involve absorption of light by the matrix. Transfer of this energy to the analyteo which then ionizes into the gas phase as a result of the relatively large amount of energy absorbed. o To accelerate the resulting ions into a flight-tube in the mass spectrometer they are subjected to a high electrical field
  • 5.  The MALDI technique combined with a MS detector (MALDI-MS) became an indispensable tool in analysis of biomolecules and organic macromolecules.  MALDI involves incorporation of the analyte into a matrix, absorption/desorption of laser radiation, and then ionization of the analyte.
  • 6.  The analyte incorporation in to a suitable matrix is the first step of the MALDI process, and is an important feature of the MALDI method.  A typical sample preparation involves using 10-6 M solution of the analyte mixed with 0.1 M solution of the matrix.  The solvents are then evaporated in a vacuum of the MS, and the matrix crystallizes with the analyte incorporated.
  • 7.  According to Sigma Aldrich, the matrix must meet the following properties and requirements : o Be able to embed and isolate analyte (e.g. by cocrystallization) o Be soluble in solvents compatible with analyte o Be vacuum stable o Absorb the laser wavelength o Cause co-desorption of the analyte upon laser irradiation o Promote analyte ionization
  • 8.  Numerous gas and solid state lasers have been developed for use in MALDI.  Most MALDI devices use a pulsed UV laser  N2 source at 337 nm.  Neodymium-yttrium aluminum garnet (Nd:YAG)  Emits at 355 nm and gives a longer pulse time  IR lasers are also used  The most common IR laser is the erbium doped-yttrium aluminum garnet (Er:YAG).  Emits at 2.94 micrometer.  It is “softer” than the UV, which is useful for certain biomolecules.
  • 9.  The typical detector used with MALDI is the time of flight mass detector (TOF-MS) o TOF is a method where the ions are accelerated by an electric field, resulting in ions of the same strength to have the same kinetic energy. o The time it takes for each ion to traverse the flight tube and arrive at the detector is based on its massto-charge ratio; therefore the heavier ions have shorter arrival times compared to lighter ions.
  • 10.  Applications of MALDI mass spectrometry in detection ofo Synthetic polymers o Peptides and proteins o Oligonucleotides o Oligosaccharides o Lipids o Inorganics o Bacterial identification
  • 11.  MALDI-MS is a vital tool in mass analysis of biomolecules and organic macromolecules.  Detection limits of femtomole to attomole.  Reproducibility is relative.  Complimentary technique to ESI (electrospray ionization)
  • 12. 1. 2. 3. 4. 5. “Matrix Assisted Laser Desorption Ionization (MALDI).” http://www.tau.ac.il/lifesci/units/proteomics/voyager.html (4/11/2013). “MALDI-TOF Mass Analysis.” http://www.protein.iastate.edu/maldi.html (4/11/2013). “Maldi Mass Spectrometry.” http://www.sigmaaldrich.com/img/assets/4242/fl_analytix6_200 1_new.pdf (4/11/2013). “MALDI Mass.” http://www.sigmaaldrich.com/analyticalchromatography/spectroscopy/maldi-mass.html (4/11/2013).

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