A Tale of Two Labs<br />Unzipping DNA<br />and<br />Molecular Motors<br />
Microtubules<br />
Kinesin<br />
Slide preparation<br />
Osmolytes<br />
Part 2<br />DNA Unzipping<br />
Unzipping DNA<br />Components:<br />Unzipping construct<br />Optical Tweezers<br />DNA to unzip<br />Optional: proteins<br />
We can unzip anything<br />F<br />F<br />For plot below:<br />Red Line – protein bound to DNA<br />Black Line – naked DNA<...
Shotgun DNA Mapping<br />Algorithm matches unzipped random digestion fragments to library of simulated unzipping fragments...
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KochLab Summary

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Here is a summary by Andy Maloney and myself. This describes what we basically do in the lab and why we have so much fun doing it. This presentation was a blast to make and I look forward to doing many more.

Credit: The song is "Missing You" by Trash80 from the album Icarus. It can be downloaded from 8bitpeoples.com.

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KochLab Summary

  1. 1. A Tale of Two Labs<br />Unzipping DNA<br />and<br />Molecular Motors<br />
  2. 2. Microtubules<br />
  3. 3. Kinesin<br />
  4. 4. Slide preparation<br />
  5. 5. Osmolytes<br />
  6. 6. Part 2<br />DNA Unzipping<br />
  7. 7. Unzipping DNA<br />Components:<br />Unzipping construct<br />Optical Tweezers<br />DNA to unzip<br />Optional: proteins<br />
  8. 8. We can unzip anything<br />F<br />F<br />For plot below:<br />Red Line – protein bound to DNA<br />Black Line – naked DNA<br />Black Dotted Line- predictions of protein locations<br />Arrows – missing protein locations<br />Unzipping can detect:<br />Naked DNA “sequence”<br />Changes to DNA sequence<br />proteins bound to DNA<br />And other possible structures<br />
  9. 9. Shotgun DNA Mapping<br />Algorithm matches unzipped random digestion fragments to library of simulated unzipping fragments<br />Initial data is based on yeast genome<br />Library of Simulated Curves<br />Experimental Force<br />Correct Match<br />
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