Marijuana and biotechnology primer

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Marijuana and biotechnology primer - Presentation Transcript

  1. Enzymatic THC A primer in biotech and marijuana Grown in marijuana or marjoram; from tobacco to tulips THC is a mere twelve carbons, thirty hydrogens and a pair of oxygens.
  2. There are four main steps in manufacturing and harvesting THC
    • Choose a host organism ie tobacco, or dandelions, or rice, or corn…
    • Add DNA from cannabis to the host
    • Feed the host a slightly special fertilizer (containing cannabigerol)
    • Harvest THC
  3. I - Choosing and growing a host
    • There are many organisms that may be suitable hosts for THC synthesis
    • We’re going to choose one we know works (tobacco, thanks to some amazing research done in Japan)
    • For best results we recommend hydroponic systems –
    • eg OmegaGardens
    • has many advantages
  4. II Adding new DNA to your host
    • The tested strategy for inserting new DNA into tobacco requires the purchase of clones built with DNA that encodes for THC Synthase
    • There are hundreds of labs capable of accomplishing this, prices range from several hundred (lowest price we’ve seen is a $295 special) to several thousand USD. Time ranges from 4 to 12 weeks.
    • The DNA is added into the roots of the tobacco plant where it becomes catalytically active.
  5. III Using a special fertilizer
    • Although cannabigerol may be added into soil the conversion rate has only been tested in a hydroponic environment
    • Over the course of seven days approximately 36% of the fertilizer is converted into THC
    • The total conversion depends largely on the size of the root system where older plants are expected to yield more THC
    Cannabigerolic Acid THC
  6. IV Harvesting
    • There are two excellent ways to harvest THC from our new tobacco
    • In a system such as the Omega Garden the liquid flowing through the roots has converted ~1/2 of your total THC
    • The remaining 50% is in the plant itself, and can be extracted through existing techniques (McKinney 1981)
SlideShare Zeitgeist 2009

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