SODIS Method


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SODIS Method

  1. 1. Solar disinfection for water purification Amanda M. Icazatti Burtell Fernando Pacheco Ocasio RISE Program University of Puerto Rico - Cayey
  2. 2. What is the SODIS Method? • Solar Disinfection for Water Purification (SODIS) • Why use the SODIS Method? • Simple procedure • Affordable • Kills pathogens • What kind of pathogens? S.C. Rivera, T.C. Hazen, G.A. Toranzos. 1988. Isolation of Fecal coliforms from pristine sites in a tropical rain forest.
  3. 3. Why is important to have purified water? Avoid diseases! •1.1 million people do not have access to safe water •Diarrhea is the second main cause of death among children under 5 years old.
  4. 4. How does the SODIS Method work? • Water sample • PET bottles • UV rays from sunlight • 6 hours full exposition • Finally, Drink purified water!
  5. 5. Important details • How recontamination is prevented? • What are some problems that the SODIS Method may confront? • Turbidity • Cloudy days • Rainy days
  6. 6. How is contamination tested in water? • Indicator • Bacteriophages • Coliphages ( E. coli) • Why use coliphages as an indicator of contamination in the water sample? • What would a positive coliphages result for E. coli indicate?
  7. 7. Problem • Will the SODIS Method be proved to reduce the presence of pathogens (coliphages) in water samples and make water safe to drink? Hypothesis • The SODIS Method will be proven with empirical evidence to reduce the presence of pathogens (coliphages) in water samples making water safe to drink.
  8. 8. Materials PET Bottles Host bacteria: E. coli Medium Broth Petri dishes Multimeter UV Rays Machine
  9. 9. Methodology Choose a place Measure water for pH, temperature, Atmospheric pressure & oxygen level Collect 800 mL of water
  10. 10. Aseptic technique Sterilize PET Bottles Transfer water sample to PET Bottles50 mL per PET Bottle
  11. 11. Prepare bacteria, medium & water (3X) 15 minutes for agar solidification Incubate at 37˚C for 24 hours
  12. 12. Results • 4 hours after incubation at 37˚C • What does the presence of bubbles indicate? pH Temperature Atmospheric pressure 8.34 24.8˚C 730.7 mmHG
  13. 13. • 19 ½ hours after incubation Control: 0 minutes Control: 10 minutes Experimental: 0 minutes Experimental: 10 minutes
  14. 14. 24 hours of incubation Control: 10 minutes Experimental: 10 minutes
  15. 15. Control Experimental
  16. 16. Conclusions • Water samples from Rio La Plata, Cayey were collected and tested for coliphages. • The SODIS Method was not performed effectively using a UV light machine. • Samples were incubated at 37˚C for a period of 24 hours. • Samples were analyzed after 4 hours, 19 ½ hours after, and 24 hours of incubation.
  17. 17. Future Work • Repeat SODIS Method using UV Rays from sunlight exposition. • Perform SODIS Method for Enterococcus to investigate if there is any human fecal pollution in Rio La Plata, Cayey.
  18. 18. Acknowledgments • We want to thanks the RISE Program for this learning experience. • Prof. Javier Arce for letting us participate in water sampling testing experiments. • Jennifer Paredes for the all the help, time dedicated, and patience. • Giovanni Cruz for letting us use the laboratory and the UV rays machine.
  19. 19. Thanks for your attention!
  20. 20. Solar disinfection for water purification Amanda M. Icazatti Burtell Fernando Pacheco Ocasio RISE Program University of Puerto Rico - Cayey