5. angelica asignment 1 february 17


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5. angelica asignment 1 february 17

  1. 1. University of Puerto Rico - Cayey RISE ProgramTemplate for Laboratory Summaries of Assignment 1 Biol. 4997-Biomedical TechniquesDue date February 17, 2012Reg.# 5 Name Angélica M. González Sánchez Date: February 12, 2012.Paragraph 1. Microscopy and PhotomicrographyIn our first laboratory we had Dr. Robert Ross as our guest teacher. He was in charge of teaching useverything concerning microscopes, micro-techniques and photomicrography. First, he defined mountingmicro-techniques used to prepare a sample for observation under a microscope. Some of thesetechniques are the whole mount, the squash, and the smear. Additionally, Dr. Ross taught us aboutmicroscopes and their purposes. We also learned about photomicrography and how to manage itsequipment. This laboratory also gave us awareness about the applications and implications ofmicroscopy, as for example to observe microorganisms for biomedical studies that could lead to a bettercomprehension of diseases caused by bacteria. To put in practice some of these applications, we made aspecial project out of the classroom. It consisted in taking a microscopic picture of an organism and lateron presenting it to our classmates further. In conclusion, in this lab I learned very useful techniqueswhich I will probably be applying in research. Overall, this experience was very satisfying because it wasboth academically enriching and entertaining.Paragraph 2. Measurements - Micropipettes and Aseptic techniques.In our second laboratory we worked with measurements, micro-pipets, Microbiology and aseptictechniques. Our objective was to manage correctly the equipment used for measurements and topromote correct aseptic techniques. First, we were instructed on the use of micro-pipets and practicedmeasuring small quantities of liquids using this equipment. Moreover, Dr. Ross taught us about thedifferent types of bacteria. He described many of their characteristics and showed us various techniquesto study them. Some of these are the isolation of a bacteria colony by making a culture with smear andthe staining of bacteria. We also learned how to sterilize the equipment used when working with micro-organisms. Along with the learning experience, we had the opportunity to practice what we learned. Weobserved bacteria slides, made a bacteria culture on our own and prepared a slide with bacteria forobservation. This laboratory helped me to review and to learn techniques which have meaningfulimplications and applications in Microbiology studies that would lead to a better understanding ofepidemic diseases.Paragraph 3. Workshop UNC – From DNA to Protein.On our UNC workshop, we learned many topics concerning DNA, proteins, and cancer. The mainobjective of this workshop was to let us know about translational medicine. During the differentconferences, we got detailed information about this area of research, which is in charge of applyingscientific knowledge for the development of new medical therapies. First of all, we learned about cancerand its terrible implications in people’s lives, such as death. Then, we had the opportunity to extract ourown DNA. We also learned about DNA, genes and oncogenes. To apply what we learned, we made apolymerase chain reaction and observed DNA with electrophoresis to see if our assigned gene wasoncogenic. Moreover, we studied proteins and oncoproteins. To practice we did an electrophoresis ofproteins and compared the results with a western blot analysis. As a whole, this workshop had severalimplications and applications. The topics and techniques presented are definitely relevant for actualbiomedical research that will lead to a deeper comprehension of diseases on which DNA and proteinsplay crucial roles, as for example in cancer.