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Im Ts Food Qa Qc Assays 07 07 2010
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Im Ts Food Qa Qc Assays 07 07 2010


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Empowering Food QA/QC teams to screen for Total Bacteria Count, Total Yeasts and Molds, E. coli, Salmonella, Listeria, in-house in about an hour.

Empowering Food QA/QC teams to screen for Total Bacteria Count, Total Yeasts and Molds, E. coli, Salmonella, Listeria, in-house in about an hour.

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  • 1. IMT’s Food QA/QC Assays Andy Moreno Vice President, Microbiology Sales Engineering Cell: +559-827-8245 FAX: +866-776-3746 [email_address] Linkedin Profile SKYPE: Andy Moreno USA Slide 1 of 8
  • 2. Industrial Microbial Testing (IMT)
      • Manufactures assays to support rapid real-time quantitative Polymerase Chain Reaction microbial testing.
        • Actionable Information: Rapid Release/99% product safe
        • Establish Positive Release Program
        • Food Safety Screening: Hold/Release time
          • ~One (1) hour/PCR vs. Traditional Culture Media Testing
          • Ingredients, In-process Solutions, Finished Product
        • Sensitivity: One (1) cell per 5 µl of sample
        • One (1) sample, many tests
    Slide 2 of 8
  • 3. Traditional Culture Media vs. ~1 HOUR qRT-PCR Real-Time Data Standard Curve versus 3 to 14 days ~ One (1) Hour Slide 3 of 8
  • 4. Slide 4 of 8
  • 5. “ Organism Prospecting Screens”
    • Water or Matrix
      • Irrigation water/Ingredients/In Process/Final Product sample
    • Total Bacterial Count (TBC) screen
      • Is there bacteria?
        • Presence/Absence
        • Quantitation
    • Total Yeasts and Molds Count (YMC) screen
      • Are there yeasts and molds?
        • Presence/Absence
        • Quantitation
    Slide 5 of 8
  • 6. IMT’s OneStep Assays 85 µl molecular grade water added to primers with PCR chemistry in dried-down format 5 µl purified DNA Sample per Cepheid Tube plus 20 µ l water and primer chemistries: total 25 µ l—4 test batch ~30 minute run time Slide 6 of 8
  • 7. Typical Food QA Station & Team
    • IMT TBC Assay and Cepheid SmartCycler
    • Minimally-trained QA/QC team(s)
    • 30 minute, easy-to-use, accurate DNA results
    Slide 5 of 7
  • 8. IMT Assays
    • Total Bioburden Assays
      • OneStep Total Bacteria Count Assay
      • OneStep Total Yeasts & Molds Count Assay
    • Commercialized Pathogen Assays
      • E. coli spp. Assay
      • Salmonella spp. Assay
      • C. botulinum “E” Assay
      • Porcine Assay
    • R&D Pipeline
      • O157:H7
      • Campylobacter Assay
      • Listeria mono. Assay
      • Enterococcus spp. Assay
      • Staphylococcus spp . Assay
      • Pseudomonas spp. Assay
      • Xanthomonas spp . Assay
      • Mycoplasma spp . Assay
    Slide of 8 Samples Total Bacteria Count Pathogen of Interest Positive Release Program
  • 9. IMT’s Assays Validate Food Producer’s Statistical Process Control Program Periodic Testing of Randomized samples against established maximum and minimum standards.
  • 10. Food: Positive Release Program
    • Irrigation water tested
    • Raw fruit and vegetables are washed and cleaned
    • Peracetic Acid and Chlorine wash bath tested at the end of each run
    • Finished product is tested
    • Environmental samples collected in plant “hot spots”
    Slide of 8
  • 11. Sample Environmental Testing* Surface Sample Collection Collect bacterial samples in filter One sample, 5 tests -Total Bacterial Count - E. coli spp. -Salmonella spp. -Listeria spp. Collection and preparation of samples = 30 minutes Quantitative real-time PCR results in 30 minutes *Quantitatively track the presence pathogens in the environment and correlate with spoilage using statistical process control methodologies.