A preliminary assessment of phytochemical constituents and antimicrobial properties in three common traditional medicinal ...
INTRODUCTION<br />  Resurgence of interest on herbal medicine and traditional   practices.<br />  FRLHT, MSSRF – agency in...
AIM  AND OBJECTIVES<br />To collect  information on common plants that are used by the community as medicine.<br />  To st...
METHODOLOGY<br />Field survey and collection of data.<br />      (a) Interviewing the practitioners and public with 			pre...
QUESTIONNAIRE (model) <br />
A mini-survey on the therapeutic uses of certain common <br />medicinal herbs at Madurai and its suburbs<br />
Binomial – Vitex negundo Linn<br />Common – Cat leaf chaste    	tree<br />Family    – Verbenaceae<br />Tamil      - Notchi...
PLANT  EXTRACTION  PROCEDURE<br />Dry coarse shoot material extracted by Soxhlet method and in iodine flask<br />Extracted...
PHYTOCHEMICALTESTS<br />
Phytochemical screening of selected plant species (End results)<br />PE -Petroleum Ether, OH –Methanol and Aq stands for A...
                  ANTIMICROBIAL ASSAY<br /> Selection of microbial culture.<br /> Preparation of Muller-Hinton agar plates...
Test for the antimicrobial activity of three selected species of medicinal plants<br />RESPONSES: <br />		-  indicate abse...
ANTIMICROBIAL  ASSAY  WITH  SELECTED  STRAINS<br />Control <br />(Staphyllococcus aureus)<br />Control (E.coli)<br />Metha...
DISCUSSION  AND  CONCLUSION<br /> Many common plants have medicinal property. <br /> Of these, the three selected plants h...
THANK YOU<br />
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Ananth vinothan

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Ananth vinothan

  1. 1. A preliminary assessment of phytochemical constituents and antimicrobial properties in three common traditional medicinal plants of Madurai<br />by<br />ANANDARAJ P (06BOT01)<br />ANANTHVINOTHAN I (06BOT02)<br />ARUN PANDI N (06BOT04)<br />Under the guidance of <br />Dr. G. C. ABRAHAM <br />
  2. 2. INTRODUCTION<br /> Resurgence of interest on herbal medicine and traditional practices.<br /> FRLHT, MSSRF – agency involved in protecting the indigenous knowledge and information.<br />
  3. 3. AIM AND OBJECTIVES<br />To collect information on common plants that are used by the community as medicine.<br /> To study the specific uses of the selected plants.<br /> To conduct phytochemical tests and an antimicrobial assay on the selected plants.<br />
  4. 4. METHODOLOGY<br />Field survey and collection of data.<br /> (a) Interviewing the practitioners and public with prepared questionnaire<br /> (b) Documentation of information in the form of tables <br />Phytochemical investigation of shoot extracts.<br /> (c) Solvent extraction <br />3. Antimicrobial assay<br />
  5. 5. QUESTIONNAIRE (model) <br />
  6. 6. A mini-survey on the therapeutic uses of certain common <br />medicinal herbs at Madurai and its suburbs<br />
  7. 7. Binomial – Vitex negundo Linn<br />Common – Cat leaf chaste tree<br />Family – Verbenaceae<br />Tamil - Notchi<br />Binomial – Coccinia indica W&A<br />Common – Ivy-gourd<br />Family – Cucurbitaceae<br />Tamil – Kovai kodi<br />Binomial – Cardiospermum halicacabum Linn<br />Common – Ballon vine, winter cherry.<br />Family – Sapindaceae<br />Tamil - Mudakkathan<br />
  8. 8. PLANT EXTRACTION PROCEDURE<br />Dry coarse shoot material extracted by Soxhlet method and in iodine flask<br />Extracted with distilled water<br />Extracted with alcohol<br />Alcoholic extract<br />Aqueous extract<br />Greenish black residue<br />Dark green residue<br />
  9. 9. PHYTOCHEMICALTESTS<br />
  10. 10. Phytochemical screening of selected plant species (End results)<br />PE -Petroleum Ether, OH –Methanol and Aq stands for Aqueous extracts.<br />
  11. 11. ANTIMICROBIAL ASSAY<br /> Selection of microbial culture.<br /> Preparation of Muller-Hinton agar plates.<br /> Spreading the microbial culture.<br /> Wells creation over the medium.<br /> Loading the wells with extracts.<br /> Analyzing the zone of inhibition after incubation.<br />
  12. 12. Test for the antimicrobial activity of three selected species of medicinal plants<br />RESPONSES: <br /> - indicate absence of any effect. <br /> +, ++ & +++ indicate the extent of the zone of inhibition in increasing order. <br />
  13. 13. ANTIMICROBIAL ASSAY WITH SELECTED STRAINS<br />Control <br />(Staphyllococcus aureus)<br />Control (E.coli)<br />Methanol extract of Coccinia showing inhibition activity over E.coli..<br />Coccinia indica W&A pet ether extract showing resistance against S.aureus growth.<br />
  14. 14. DISCUSSION AND CONCLUSION<br /> Many common plants have medicinal property. <br /> Of these, the three selected plants have some special constituents.<br /> Among the selected strains, S.aureus (G+) is controlled more than E.coli (G-).<br />Of the three plants, Coccinia is more effective than the other two.<br />Among the extracts, organic is more effective than aqueous and in that methanol is better than per ether extract.<br />
  15. 15. THANK YOU<br />
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