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  • 1. A preliminary assessment of phytochemical constituents and antimicrobial properties in three common traditional medicinal plants of Madurai
    by
    ANANDARAJ P (06BOT01)
    ANANTHVINOTHAN I (06BOT02)
    ARUN PANDI N (06BOT04)
    Under the guidance of
    Dr. G. C. ABRAHAM
  • 2. INTRODUCTION
    Resurgence of interest on herbal medicine and traditional practices.
    FRLHT, MSSRF – agency involved in protecting the indigenous knowledge and information.
  • 3. AIM AND OBJECTIVES
    To collect information on common plants that are used by the community as medicine.
    To study the specific uses of the selected plants.
    To conduct phytochemical tests and an antimicrobial assay on the selected plants.
  • 4. METHODOLOGY
    Field survey and collection of data.
    (a) Interviewing the practitioners and public with prepared questionnaire
    (b) Documentation of information in the form of tables
    Phytochemical investigation of shoot extracts.
    (c) Solvent extraction
    3. Antimicrobial assay
  • 5. QUESTIONNAIRE (model)
  • 6. A mini-survey on the therapeutic uses of certain common
    medicinal herbs at Madurai and its suburbs
  • 7. Binomial – Vitex negundo Linn
    Common – Cat leaf chaste tree
    Family – Verbenaceae
    Tamil - Notchi
    Binomial – Coccinia indica W&A
    Common – Ivy-gourd
    Family – Cucurbitaceae
    Tamil – Kovai kodi
    Binomial – Cardiospermum halicacabum Linn
    Common – Ballon vine, winter cherry.
    Family – Sapindaceae
    Tamil - Mudakkathan
  • 8. PLANT EXTRACTION PROCEDURE
    Dry coarse shoot material extracted by Soxhlet method and in iodine flask
    Extracted with distilled water
    Extracted with alcohol
    Alcoholic extract
    Aqueous extract
    Greenish black residue
    Dark green residue
  • 9. PHYTOCHEMICALTESTS
  • 10. Phytochemical screening of selected plant species (End results)
    PE -Petroleum Ether, OH –Methanol and Aq stands for Aqueous extracts.
  • 11. ANTIMICROBIAL ASSAY
    Selection of microbial culture.
    Preparation of Muller-Hinton agar plates.
    Spreading the microbial culture.
    Wells creation over the medium.
    Loading the wells with extracts.
    Analyzing the zone of inhibition after incubation.
  • 12. Test for the antimicrobial activity of three selected species of medicinal plants
    RESPONSES:
    - indicate absence of any effect.
    +, ++ & +++ indicate the extent of the zone of inhibition in increasing order.
  • 13. ANTIMICROBIAL ASSAY WITH SELECTED STRAINS
    Control
    (Staphyllococcus aureus)
    Control (E.coli)
    Methanol extract of Coccinia showing inhibition activity over E.coli..
    Coccinia indica W&A pet ether extract showing resistance against S.aureus growth.
  • 14. DISCUSSION AND CONCLUSION
    Many common plants have medicinal property.
    Of these, the three selected plants have some special constituents.
    Among the selected strains, S.aureus (G+) is controlled more than E.coli (G-).
    Of the three plants, Coccinia is more effective than the other two.
    Among the extracts, organic is more effective than aqueous and in that methanol is better than per ether extract.
  • 15. THANK YOU