1. Microbiology Lecture 1 Dr. Saleh M Y OTH PhD Medical Molecular Biotechnology and Infectious Diseases 02/10/2010 IMS - MSU
2. Microbiology is the science that deals with tiny organisms that can’t be seen by naked eye. -micro=tiy , -bio=alive , - logy=science Introduction In general; we call this part of the science : Microbiology and the organisms called Microorganisms These microorganisms are widley distributed in nature and some of them are beneficial to man and some of them are harmful (pathogenic; cause diseaes) .
3. In our field of medical microbiolgy , we deal with microbs that cause diseases to man. Microbiology Viruses = Virulogy Bacteria = Bacteriolgy Fungi = Mycology Parasites = Parasitology Immunity = Immunology Antibiotics = in use for treament Medical Microbiology Food Microbiology Water Microbiology Air Microbiology Soil Microbiology Space Microbiology Industrial Microbiology Pranches of Microbiology Algae = Phycology Prtotzoa = Protozoology
4. The harmful microbs (agents) called pathogens Thus; the agents of human infecious diseases belong to 5 major groups: 1- Viruses 2- Bcateria 3- Fungi 4- Protozoa 5- Helminths Parasites Viruses are quite distinct from the other organisms as they are NOT cells but can replicate only within cells.
5. BACTERIA are microscopic unicellular Prokaryotes, the MOST Numerous Organisms on the Earth Evolution and Classification of Bacteria Bacteria have evolved into many different forms, and they are now part of nearly every environment on the Earth. They have been found at the bottom of the oceanic trenches 9.6 km (6 mi) below the water's surface and in Arctic and Antarctic Regions.
6. Evidence in the fossil record indicates that Prokaryotes are about 2.5 Billion Years Old and Modern Humans arose about 100,000 years ago. Unlike most other organisms, Bacteria have few Morphological differences that can be used to classify them. Bacteria do Not vary in size and shape to the extent that other types of organisms do. Classicaly, bacteria have been grouped based on their Structure , Physiology , Molecular Composition , and Reaction to specific types of stains (Gram Stain), rather than on their evolutionary relationships.
7. Bacteria can be one of THREE Different SHAPES: The three common types of bacterial morphology; Cocci - coccus , Bacilli - bacillus , spirillum These types: spherical, rod and sprial shaped morphologies are typical of such genera as: Streptococcus / Staphylococcus , Escherichia / Bacillus and Leptospira / Spirillum .
9. <ul><li>Bacteria are all around us. </li></ul><ul><li>Given good growing conditions, a bacterium grows slightly in size or length. </li></ul><ul><li>A new cell wall grows through the center forming two daughter cells, each with the same genetic material as the parent cell. </li></ul><ul><li>If the environment is optimum, the two daughter cells may divide into four in 20 minutes. </li></ul><ul><li>That mins: 1, 2, 4, 8, 16, 32, 64... Then why isn't the earth covered with bacteria...? </li></ul>Bacteria: growth, divide and multiply
10. <ul><li>The primary reason may be that conditions are rarely optimum. </li></ul><ul><li>Scientists who study bacteria try to create the optimum environment in the lab: culture medium with the necessary energy source, nutrients, pH, and temperature, in which bacteria grow predictably. </li></ul>Bacteria: growth, divide and multiply
11. 1- LAG PHASE: Growth is slow at first, while the "bugs" acclimate to the food and nutrients in their new habitat. 1 2 3 4 2- LOG PHASE: Once the metabolic machinery is running, they start multiplying exponentially, doubling in number every few minutes. 3- STATIONARY PHASE: As more and more bugs are competing for dwindling food and nutrients, booming growth stops and the number of bacteria stabilizes. 4- DEATH PHASE: Toxic waste products build up, food is depleted and the bugs begin to die. Growth Curve
12. STRUCTURE of the BACTERIAL CELL
13. Bacteria are Typically composed of: Cell Wall, Cell Membrane, and Cytoplasm Some bacteria have Distinctive Structures, such as Endospores , Capsules , and Outer Membranes . The Variety of Structures among Bacteria is due to adaptations to individual to take its place in the world.
17. CELL WALL bacteria have a Cell Wall and its function is to protect the bacterial cells from bursting or destroys and gives the bacteria its shape.
18. <ul><li>CELL MEMBRANE AND CYTOPLASM </li></ul><ul><li>The Bacterial Cell Membrane is composed of a Lipid Bilayer similar to Eukaryotes. </li></ul><ul><li>2. In Bacteria, the cell membrane contains Enzymes that Perform the Reactions of Cellular Respiration. </li></ul><ul><li>3. Bacteria do NOT have Mitochondria so they use their cell membranes to carry out Cellular Respiration. </li></ul><ul><li>4. Bacterial cells do NOT have membrane bound organelles. The CYTOPLASM of Bacterial cells is made of a Viscous (Thick Liquid) solution of Ribosomes and DNA. </li></ul><ul><li>5. The Bacterial DNA is arranged in a single, closed loop. Some bacteria also have PLASMIDS, self-replicating loops of DNA, in their Cytoplasm. </li></ul>
19. <ul><li>Capsules and Pili </li></ul><ul><li>Many bacterial species produce an Outer Covering called a CAPSULE . The capsule is made of polysaccharides that cling to the surface of the cell and Protect It against drying or harsh chemicals and a host body's white blood cells, which would otherwise engulf (eat) it. </li></ul><ul><li>2. When a Capsule consists of a Fuzzy Coat of Sticky Sugars, it is called a GLYCOCALX (GLIE-koh-KAY-liks). The Glycocalyx enables bacteria to attach to the surface of host cells and tissue. </li></ul><ul><li>3. PILI are Short, Hairlike Protein Structures found on the Surface of some species of bacteria. Pili help bacteria hold on to host cells, and are also used to transfer genetic material from one bacterium to another. </li></ul>
20. STRUCTURAL CHARACTERISTICS OF A BACTERIAL CELL moves the cell Flagellum assist the cell in attaching to other surfaces, which is important for genetic recombination Pilus (Pili) protects the cell against harsh environmental conditions, such as heat or drought Endospore protects the cell and assist in attaching the cell to other surfaces Capsule, and slime layer contains some genes obtain through genetic recombination Plasmid carries genetic information inherited from past generations Chromosome contains DNA, ribosomes, and organic compounds required to carry out life processes Cytoplasm regulates movement of materials into and out of the cell; contains enzymes important to cellular respiration Cell Membrane protects the cell against some antibiotics (only present in Gram-negative cells) Outer Membrane protects the cell and gives shape Cell Wall FUNCTION STRUCTURE