mbbs ims msu

1
WBC Count
Specific Equipment
1. White pipette
2. Rubber pipe
3. Plastic syringe
4. Pipette vibrator
5. Hemacytometer & cover slip
(Counting chamber)
6. Microscope
7. Counter (hand tally)
8. Report form

WBC Count
Reagent
WBC diluting fluids
1. Turk’s solution
Glacial acetic acid
Gential violet
Distilled water
2. Two % acetic acid
3. One % hydrochloric acid

3
WBC Count
Specimens
1. Fresh blood
2. Anticoagulated blood
EDTA
Double Oxalate
Citrate
Heparin
Etc.

WBC Count
Procedure
1. Mix the blood sample thoroughly.
2. Fill blood into white pipette at 0.5 mark.
3. Fill reagent add up into the pipette
to 11 mark.
4. Shake the pipette on the vibrator for 1 min.
5. Discarded the first 3-4 drops.
6. Fill in the hemacytometer nicely.
7. Allow WBC to set down for 2-3 min.
8. Count 4 white squares under microscope
9. Calculate the WBC concentration.

5
WBC Count
1 mm.
W
W
1 mm.
R
R
1 mm.
R
R
R
W
W
High 0.1 mm.
3 mm.

6
WBC Count
W
1 mm.
High 0.1 mm.
1mm.

7
WBC Count Calculation
1. Volume of 1 white square = 1 x 1 x 0.1 cu.mm.
= 0.1 cu.mm.
2. Volume of 4 white square = 0.1 x 4 cu.mm.
= 0.4 cu.mm.
3. In 0.4 cu.mm. the WBC count = N (counted No.)
4. In 1 cu.mm. the WBC count = N x 1 / 0.4
= N x 2.5
5. The dilution for WBC = 0.5 / 10
= 20
6. The final WBC count = N x 2.5 x 20
= 50 N (/cu.mm.)

8
WBC Count
Calculation
White cell count = number of cells counted (N)
x volume factor (=2.5)
x dilution factor (=20)
= N x 50
Normal range = 5,000 - 10,000 / cu.mm.
5 - 10 x 103 / cu.mm.

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