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mbbs ims msu
 

mbbs ims msu

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int asst practical

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    mbbs ims msu mbbs ims msu Presentation Transcript

    • 1
      RBC Count
    • 2
      RBC Count
      Number of red blood cells per a unit volume
      (x million / cu.mm.)
      ( x 106 / cu.mm.)
      (x 106 / mm3.)
      (x 106 / uL.)
      (x 1012 / L.)
    • 3
      RBC Count (cont.)
      Requirement
      1. Blood collection and diluting system
      2. Rubber pipe
      3. Plastic syringe
      4. Pipette vibrator
      5. Hemacytometer & cover slip
      (Counting chamber)
      6. Microscope
      7. Counter (hand tally)
      8. Report form
    • 4
      Specimens
      1. Fresh blood
      2. Anticoagulated blood
      EDTA
      Double Oxalate
      Citrate
      Heparin
      Etc.
    • 5
      Procedure
      A finger is lanced and small amount of blood is allowed to accumulate
      Blood is drawn into the pipette
      Fill blood into red pipette at 0.5 mark.
      Fill reagent add up into the pipette to 101 mark.
      Shake the pipette on the vibrator for 1 min.
    • Procedure
      Discarded the first 3-4 drops.
      Fill in the hemacytometer nicely.
      Allow RBC to set down for 2-3 min.
      Count 5 red squares under microscope
      Calculate the RBC concentration.
      6
    • 7
      RBC Count (cont.)
      Counting
      1. Count in 5 red cell squares in the middle
      (In 4 squares at corners and another 1 in the middle).
      2. Top-left touching count
      3. Using counter hand tally or hand tally
    • 8
      RBC Count (cont.)
      0.2 mm.
      W
      W
      R
      R
      1 mm.
      R
      0.2 mm.
      R
      R
      W
      W
      High 0.1 mm.
      3 mm.
    • 9
      RBC Count (cont.)
      Top
      Left
    • 10
      RBC Count (cont.)
      Calculation
      Red cell count = number of cells counted (N)
      x volume factor (=50)
      x dilution factor (=200)
      = N x 10,000
      Normal range = 3.8 - 6.0 x 106 / cu.mm.
    • Decrease in RBC
      Burns
      Pregnancy
      Anemia
      Iron deficiency
      Vit B12
      Bone marrow damage
      11
    • Increased RBC
      Dehydration
      Heart disease
      Renal problems
      12