mbbs ims msu

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mbbs ims msu

  1. 1. 1<br />RBC Count <br />
  2. 2. 2<br />RBC Count<br />Number of red blood cells per a unit volume <br />(x million / cu.mm.) <br />( x 106 / cu.mm.)<br />(x 106 / mm3.)<br />(x 106 / uL.)<br />(x 1012 / L.)<br />
  3. 3. 3<br />RBC Count (cont.)<br />Requirement<br />1. Blood collection and diluting system <br />2. Rubber pipe<br />3. Plastic syringe<br />4. Pipette vibrator<br />5. Hemacytometer & cover slip<br /> (Counting chamber)<br />6. Microscope<br />7. Counter (hand tally)<br />8. Report form<br />
  4. 4. 4<br />Specimens<br />1. Fresh blood<br />2. Anticoagulated blood<br /> EDTA<br /> Double Oxalate<br /> Citrate<br /> Heparin<br /> Etc.<br />
  5. 5. 5<br />Procedure<br />A finger is lanced and small amount of blood is allowed to accumulate<br />Blood is drawn into the pipette<br />Fill blood into red pipette at 0.5 mark.<br />Fill reagent add up into the pipette to 101 mark.<br />Shake the pipette on the vibrator for 1 min.<br />
  6. 6. Procedure<br />Discarded the first 3-4 drops.<br />Fill in the hemacytometer nicely.<br />Allow RBC to set down for 2-3 min.<br />Count 5 red squares under microscope <br />Calculate the RBC concentration.<br />6<br />
  7. 7. 7<br />RBC Count (cont.)<br />Counting<br />1. Count in 5 red cell squares in the middle<br /> (In 4 squares at corners and another 1 in the middle). <br />2. Top-left touching count<br />3. Using counter hand tally or hand tally<br />
  8. 8. 8<br />RBC Count (cont.)<br />0.2 mm.<br />W<br />W<br />R<br />R<br />1 mm.<br />R<br />0.2 mm.<br />R<br />R<br />W<br />W<br />High 0.1 mm.<br />3 mm.<br />
  9. 9. 9<br />RBC Count (cont.)<br />Top<br />Left<br />
  10. 10. 10<br />RBC Count (cont.)<br />Calculation<br />Red cell count = number of cells counted (N)<br /> x volume factor (=50)<br /> x dilution factor (=200)<br /> = N x 10,000<br />Normal range = 3.8 - 6.0 x 106 / cu.mm.<br />
  11. 11. Decrease in RBC<br />Burns<br />Pregnancy<br />Anemia<br />Iron deficiency<br />Vit B12<br />Bone marrow damage<br />11<br />
  12. 12. Increased RBC<br />Dehydration<br />Heart disease<br />Renal problems<br />12<br />

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