Meet the T100 from Bio-Rad Laboratories


Published on

The Bio-Rad T100 thermal cycler makes PCR a truly touching experience. With its compact footprint, reliable performance and easy-to-use touch screen, running PCR has never been easier.

1 Like
  • Be the first to comment

No Downloads
Total views
On SlideShare
From Embeds
Number of Embeds
Embeds 0
No embeds

No notes for slide

Meet the T100 from Bio-Rad Laboratories

  1. 1. T100 TM Thermal Cycler 7/5/2011
  2. 2. Bio-Rad Thermal Cyclers <ul><li>Bio-Rad offers a wide variety of thermal cyclers from personal 48-well cyclers to high-throughput multi-bay instruments </li></ul>Tetrad 2 Dyad C1000 Touch S1000 T100 MJ Mini
  3. 3. <ul><li>The T100 is an easy-to-use, 96-well gradient thermal cycler with reliable performance from a market leader in PCR </li></ul>T100 Thermal Cycler
  4. 4. <ul><li>Save time programming with the intuitive touch screen </li></ul><ul><li>Get superior results faster by optimizing your PCR assays in a single run using a thermal gradient </li></ul><ul><li>Save valuable benchspace with the compact design </li></ul><ul><li>Keep your protocols organized using personalized folders or a USB flash drive </li></ul><ul><li>Be confident in you results with the reliability you expect from Bio-Rad </li></ul>Key Features & Benefits
  5. 5. <ul><li>Save time programming and training new users </li></ul><ul><li>Instant Incubate feature is convenient for maintaining constant temperatures for quick ligations or digests </li></ul><ul><li>Library of pre-installed protocols for routine applications allows new users to get started immediately </li></ul>Intuitive Touch Screen
  6. 6. <ul><li>Custom folders keep protocols organized for multiple users </li></ul><ul><li>Preview panel makes it easy to find a protocol </li></ul><ul><li>Easily backup protocols on a PC for peace of mind </li></ul><ul><li>Save time transferring protocols between T100 thermal cyclers with the USB drive </li></ul>Custom Folders & USB Drive
  7. 7. <ul><li>Gradient allows you to optimize a reaction for maximum yield and specificity in a single run, saving time and money on reagents </li></ul><ul><li>Dynamic ramping ensures that hold times are identical across rows of a gradient </li></ul>Thermal Gradient with Dynamic Ramping
  8. 8. <ul><li>Sealed electronics ensure long-lasting, reliable performance. </li></ul><ul><li>Backed by a company with over 20 years of experience in PCR. </li></ul>Reliable Performance Premium thermal cyclers Personal thermal cyclers 1988: PTC100 1991: MiniCycler 1994 DNA Engine 1995 Tetrad 1999 iCycler 2008 1000-series 2002 MyCycler 2005 MJ Mini 2011 T100 2011 C1000 Touch 2000 Dyad
  9. 9. <ul><li>Accuracy: ± 0.5 ° C </li></ul><ul><li>Uniformity: ± 0.5 ° C </li></ul><ul><li>Maximum ramp rate: 4 ° C/s </li></ul><ul><li>Average ramp rate: 2.5 ° C/s </li></ul><ul><li>Sample volumes: 1-100 μ L </li></ul><ul><li>Temperature range: 4-100 ° C </li></ul>Reliable Performance Consistent results can be obtained at low (5 μL) sample volumes. A 370 bp target was amplified from human genomic DNA using iTaq supermix and the product from each well was run on a gel
  10. 10. <ul><li>Standby mode turns off the screen, saving energy </li></ul><ul><li>Pre-installed protocols for fast PCR further reduce energy usage </li></ul><ul><li>Beep notifies you that the run is finished so you can shut off the cycler immediately, saving more energy </li></ul>Energy Efficient
  11. 11. <ul><li>Choice of full-height PCR plastics (tubes, tube strips, or plates)* </li></ul><ul><ul><li>MLP9601 - 96 well unskirted microplate </li></ul></ul><ul><ul><li>HSS9601 - 96 well semi-skirted plates </li></ul></ul><ul><ul><li>MSB1001 - Microseal B </li></ul></ul><ul><li>A complete system with our best in-class reagents </li></ul><ul><ul><li>170-8870 - iTaq DNA Polymerase </li></ul></ul><ul><ul><li>172-5301 - iProof for high fidelity PCR </li></ul></ul><ul><ul><li>170-8890 - iScript select cDNA Synthesis kit </li></ul></ul>PCR Plastics Compatibility and Reagents * Does not accept low-profile plastics, or full-skirted plates
  12. 12. <ul><li>So simple that training is not required </li></ul>Home screen
  13. 13. <ul><li>Touch any field to edit it via a popup keypad </li></ul><ul><li>Touch Insert to insert a step </li></ul><ul><li>Touch Options to change the options and insert a gradient </li></ul>New Protocol
  14. 14. <ul><li>Touch Save to input a name and a folder to save the protocol </li></ul><ul><li>Touch Run to start running immediately </li></ul>New Protocol
  15. 15. <ul><li>Status view shows the details of your run in progress </li></ul><ul><li>Touch View Clock to see the time remaining enlarged so you can monitor a run from across the lab </li></ul>Status
  16. 16. <ul><li>Select a protocol, then touch Run </li></ul><ul><ul><li>Preview allows you to easily find the right protocol </li></ul></ul><ul><ul><li>RECENT folder shows the last 20 runs for quick repeats </li></ul></ul><ul><ul><li>MAIN folder has standard pre-installed protocols </li></ul></ul>Saved Protocols
  17. 17. <ul><li>Insert a USB drive for easy protocol transfer and backup on a PC </li></ul><ul><li>Touch Folder Options > New to create a new folder, making it easy to organize protocols </li></ul>Saved Protocols
  18. 18. <ul><li>Touch Incubate from the Home screen </li></ul><ul><li>Convenient for keeping samples at the same temperature for ligations or digests, like a heat block </li></ul><ul><li>Set the desired temperature, then touch Run </li></ul>Incubate
  19. 19. Appendix – Touch screen information <ul><li>Are touch screens reliable? </li></ul><ul><ul><li>Yes. Bio-Rad did not adopt touch screens when the technology was new and unproven </li></ul></ul><ul><ul><li>Now touch screens are common and accepted as being reliable for phones, GPS devices and lab equipment </li></ul></ul><ul><ul><li>The T100 touch screen has been tested with over 1 million touches </li></ul></ul><ul><li>Can I wear gloves? </li></ul><ul><ul><li>Yes. The touch screen on the T100 can be used with or without gloves </li></ul></ul>
  20. 20. Appendix - Ordering Information Domed 12-Cap Strips , for 0.2 ml PCR tubes and plates, natural, 200 TCS-1201 12-Tube Strips without Caps (0.2 ml), natural, 100 strips (1,200 PCR tubes) TBS-1201 Microseal® 'B' Adhesive Seals , optically clear, 100 MSB-1001 Multiplate™ 96-Well Unskirted PCR Plates, natural, 25 plates MLP-9601 Hard-Shell® Full-Height 96-Well Semi-Skirted PCR Plates , clear shell, clear well, 25 HSS-9601 iProof High-Fidelity DNA Polymerase , 2 U/μl, 100 U, includes 5x reaction buffers, MgCl2 solution, DMSO 172-5301 iTaq DNA Polymerase , 5 U/μl, includes 250 U polymerase, 1.25 ml 10x PCR buffer (200 mM Tris-HCl, pH 8.4, 500 mM KCl), 1.25 ml 50 mM MgCl2 solution 170-8870 iScript cDNA Synthesis Kit , 25 x 20 μl reactions, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water iScript Select cDNA Synthesis Kit, 25 x 20 μl reactions, primers in separate tubes iScript Supermix , 25 x 20 μl reactions, one-tube formulation, includes no RT control iScript Advanced, 50 x 20 μl reactions, up to 7.5 μg input RNA 170-8890 170-8896 170-8840 170-8842 T100 Thermal Cycler , includes 96-well thermal cycler, power cord, tube support ring 186-1096 Description Part Number