DNA Extractionhttp://gslc.genetics.utah.edu/units/activities
MATERIALS•   Dried split peas•   Dishwashing detergent•   Isopropyl alcohol•   Meat tenderizer•   Small cup or beaker•   S...
Start with the peas
Blend the peas with water and alittle salt until you have “pea soup”
Why do we blend the peas?• The blender separates the pea CELLS  from each other
Put the strained pea soup in a cup   Add a squirt of dishwashing           detergent
Let the mixture sit for 5        minutes
What does the detergent do?• Each pea cell is  surrounded by a cell  membrane• DNA is in the nucleus  of a cell, which is ...
The membranes must bebroken open in order to get the     DNA out of the cell
Detergent:• Breaks apart membranes by attaching to the  lipids (fats) & proteins in the membranes
The cell and nuclear membranes have been                 broken apart, as well as all of the organelle membranes,such as t...
Add a pinch of meat tenderizer to          the mixture
Why?• Meat tenderizer is an ENZYME  – A protein that helps chemical reactions    happen more quickly• The enzymes in meat ...
The DNA in the nucleus is surrounded by proteins
The meat tenderizer cuts away  the proteins from the DNA
Stir very, very GENTLY!
Tilt your cup & slowly pour a layer of alcohol on top of the pea soup               mixture• DNA will float up into the al...
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Dna extraction 2

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Dna extraction 2

  1. 1. DNA Extractionhttp://gslc.genetics.utah.edu/units/activities
  2. 2. MATERIALS• Dried split peas• Dishwashing detergent• Isopropyl alcohol• Meat tenderizer• Small cup or beaker• Spoon
  3. 3. Start with the peas
  4. 4. Blend the peas with water and alittle salt until you have “pea soup”
  5. 5. Why do we blend the peas?• The blender separates the pea CELLS from each other
  6. 6. Put the strained pea soup in a cup Add a squirt of dishwashing detergent
  7. 7. Let the mixture sit for 5 minutes
  8. 8. What does the detergent do?• Each pea cell is surrounded by a cell membrane• DNA is in the nucleus of a cell, which is also surrounded by a membrane
  9. 9. The membranes must bebroken open in order to get the DNA out of the cell
  10. 10. Detergent:• Breaks apart membranes by attaching to the lipids (fats) & proteins in the membranes
  11. 11. The cell and nuclear membranes have been broken apart, as well as all of the organelle membranes,such as those around the mitochondria and chloroplasts. So what is left?• Proteins• Carbohydrates (sugars)• DNA
  12. 12. Add a pinch of meat tenderizer to the mixture
  13. 13. Why?• Meat tenderizer is an ENZYME – A protein that helps chemical reactions happen more quickly• The enzymes in meat tenderizer break apart proteins
  14. 14. The DNA in the nucleus is surrounded by proteins
  15. 15. The meat tenderizer cuts away the proteins from the DNA
  16. 16. Stir very, very GENTLY!
  17. 17. Tilt your cup & slowly pour a layer of alcohol on top of the pea soup mixture• DNA will float up into the alcohol from the pea soup
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