Your SlideShare is downloading. ×
Gene therapy
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×

Saving this for later?

Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime - even offline.

Text the download link to your phone

Standard text messaging rates apply

Gene therapy

369
views

Published on

gene therapy, vectors

gene therapy, vectors

Published in: Education, Technology

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
369
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
25
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. By, D.Akshaya B.GOKULA PRIYA
  • 2.  GENE THERAPY- DEFINITION  HISTORY-GENE THERAPY  TECHNIQUES AND TERMS  TYPES OF GENE THERAPY  ADVANTAGES AND DISADVANTAGES  CONCLUSION
  • 3. Gene therapy is the use of DNA as a pharmaceutical agent to treat disease.
  • 4.  DNA: Genes are composed of DNA, a molecule in the memorable shape of a double helix, a spiral ladder. Each rung of the spiral ladder consists of two paired chemicals called bases. There are four types of bases. They are adenine (A), thymine (T), cytosine (C), and guanine (G). As indicated, each base is symbolized by the first letter of its name: A, T, C, and G. Certain bases always pair together (AT and GC). Different sequences of base pairs form coded messages.  The gene: A gene is a sequence (a string) of bases. It is made up of combinations of A, T, C, and G. These unique combinations determine the gene's function, much as letters join together to form words. Each person has thousands of genes -- billions of base pairs of DNA or bits of information repeated in the nuclei of human cells -- which determine individual characteristics (genetic traits).
  • 5.  The concepts of gene therapy arose initially during the 1960s and early 1970s ….  With the arrival of recombinant DNA techniques, cloned genes became available  Efficient retroviral vectors and other gene transfer methods have permitted convincing demonstrations of efficient phenotype correction in vitroand in vivo, now making gene therapy a broadly accepted approach to therapy
  • 6. PICTORIAL REPRESENTATION..
  • 7.  Cells from the patient’s blood or bone marrow are removed (usually cells containing defective genes)  The cells are exposed to viruses,carrying the desired genes…  Then,the cells are grown in the laboratory and then injected into the patient’s body.
  • 8.  Viral method-uses viral vectors for gene transfer…  Method: Viruses are first disabled to replicate themselves and are exposed to the cells  Drawback:possiblities for vial multiplication- leads to new diseases..  NON-VIRAL METHODS  Electroporation- electric field  Sonoporation- ultasonic frequencies  Gene gun- bombardment  Magnetofection- magnetic field
  • 9.  SOMATIC -gene therapy  Gene transfer to somatic cells…  The effect is not hereditary i.e confined to the individual  GERM LINE-gene therapy  Gene transfer to germ cells viz..sperm or egg  The effect is hereditary
  • 10. Genetic disorders  Severe Combined Immune Deficiency  Chronic Granulomatus Disorder  Hemophilia Acquired diseases  Cancer  Neurodegenerative diseases  HIV
  • 11. Advantages arrests some diseases  gives patients new genes, so they don't have the disease and the disease doesn't come back  provides as a way of treatment when in the past the disease would have been incurable  there have been several medical breakthroughs  some people consider it a great advancement for mankind Disadvantages In the past, gene therapy has cured some diseases, but caused others  Its inconsistent  Its a fairly new method, so there are still some glitches to it that scientists are still figuring out.  some people are against the ethics of it: some say its "playing God"  the patient may have to undergo several therapy treatments
  • 12.  GENE-THERAPY is an upcoming field and in the future i.e within a decade its going to be the cure for every genetic disease and the other methods would go a cliche!!