Your SlideShare is downloading. ×
0
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Tangential flow filtration
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Tangential flow filtration

1,026

Published on

Tangential Flow Filtration [TFF] is used for clarifying, concentrating and purifying proteins. …

Tangential Flow Filtration [TFF] is used for clarifying, concentrating and purifying proteins.

Filtration is a pressure driven separation process that uses a membrane to separate components in liquid solution or suspension based on size and charge difference.

Types of Membrane Filtration

1. Normal Flow Filtration - Dead End Filtration
2. Tangential Flow Filtration - Cross Flow Filtration

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
1,026
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
1
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. Tangential Flow Filtration: Membrane Types and Process Parameters http://technologyinscience.blogspot.com
  • 2. • Tangential Flow Filtration [TFF] is used for clarifying, concentrating and purifying proteins. • Filtration is a pressure driven separation process that uses a membrane to separate components in liquid solution or suspension based on size and charge difference.
  • 3. Types of Membranes • 1. Normal Flow Filtration - Dead End Filtration • 2. Tangential Flow Filtration - Cross Flow Filtration
  • 4. Types of TFF • 1. Micro Filtration • 2. Ultra Filtration Ultra Filtration is catagorized into two • Virus Filtration • High Performance Tangential Flow Filtration
  • 5. Process Goals • Final Product Concentration • Feed Volume Reduction • Extent of Buffer Exchange • Contaminant Removal Specification
  • 6. Primary Protein Processing Goals • High Product Yield • High Product Quality • High Product Purity • Controlled Bioburden
  • 7. TFF Membranes • Polyethersulfone (PES) • Regenerated Cellulose
  • 8. Types of TFF Membranes • Flat Plate • Spiral Wound • Hollow Fiber
  • 9. Key Parameters to Optimize • Cross Flow Rate • Transmembrane Pressure • Filtrate Control • Membrane Area • Diafiltration Design
  • 10. Factors affecting Product Yield These are the four contribution of product loss during Tangential Flow Filtration • Retention Loss • Adsorption Loss • Solubility Loss • Unrecoverable hold up volume loss
  • 11. Factors affecting Product Quality Quality of product is compromised due to aggregation or denaturation caused by • Micro cavitation • Air-Liquid Interface • High Protein Concentration • Temperature Effects
  • 12. Thank You • Click here to Visit Bio-Resource for more details on Tangential Flow Filtration • http://technologyinscience.blogspot.com

×