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Mechanism of interaction of Ethidium Bromide (EtBr) with DNA

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ajithnandanam

Ethidium Bromide contains tricyclic phenanthridine ring system that is able to interact with stacked base pairs of double stranded DNA. Ethidium is capable of forming close van der Walls contacts with the base pairs and due to that it can bind to the hydrophobic interior of the DNA molecule. The peripheral phenyl and ethyl groups projects into the major groove of DNA helix.

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Mechanism of Interaction Ethidium Bromide with DNA Bio-Resource www.technologyinscience.blogspot.com
Ethidium Bromide • Ethidium Bromide (EtBr) is an intercalating agent widely used in molecular biology labs as a fluorescent dye for nucleic acid gel electrophoresis www.technologyinscience.blogspot.com
Structure of Ethidium Bromide www.technologyinscience.blogspot.com
Interaction of EtBr with DNA www.technologyinscience.blogspot.com
Ethidium Bromide Stained Gel www.technologyinscience.blogspot.com
EtBr Toxicity and Alternatives • Gel Red • SYBR Safe www.technologyinscience.blogspot.com

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