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micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
micro propagation of banana
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micro propagation of banana

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  • 1. MICROPROPAGATION FOR PRODUCTION OF QUALITY BANANA PLANTING MATERIAL SPEAKER Mr. AMOL V. JAMALEReg. No: J4-00905-2011PGIABM, JAU, JUNAGADH 1
  • 2. CONTENT Introduction. Why do micropropagation? Advantages of micropropagation. General steps of micropropagation. Varieties grown in India. Economic of micropropagated banana. SWOT analysis of micropropagation. Government schems and incentives. Conclusion. 2
  • 3. INTRODUCTION  Banana is a globally important fruit crop with 97.5 million tones of production.  India produces about 26.217 million tones of Banana from an area of 0.709 Mha., with an average productivity of 37.0 mt/ha.  Banana occupy 20% area among the total area under crop in India.  Micropropagation is refers to rapid asexual in vitro multiplication of a desired plant of banana.  In India only 7 percent area under the tissue culture plantlets and remaining 93percent propagated by sucker.  New entrepreneurs having sound scope in production of banana micropropagated plantlets.Source :- www.apcoab.org 3
  • 4. LEADING BANANA PRODUCING COUNTRIESSource: National Horticulture Board (NHB) 2009-10 4
  • 5. Indian ProductionState wise Production of Banana in of BananaIndiaSource: National Horticulture Board (NHB) 2009-10 5
  • 6. DISTRICT WISE ESTIMATED AREA, PRODUCTION & PRODUCTIVITY OF BANANA IN GUJARAT YEAR 2010-11 AREA IN HECTORS, PRODUCTION IN M.T., PRODUCTIVITY M.T./HA. GUJARAT STATESr. Area Productio ProductiviNo. Name of District n ty 1 Bharuch 15200 990280 65.15 2 Anand 14045 877391 62.47 3 Baroda 9390 569130 60.61 4 Narmada 6000 384000 64.00 5 Junagadh 1800 81000 45.00 6 Bhavnagar 1600 88000 55.00 7 Tapi 1600 88000 55.00 8 Kutch 1000 52690 52.69 9 Baroda 9390 569130 60.61 10 Kheda 806 36270 45.00 11 Valsad 770 43120 56.00 12 Navsari 460 25760 56.00 13 Panchmahal 300 9150 30.50 14 Sabarkantha 204 6149 30.14Source:- agri.gujarat.Gov. in 6
  • 7. WHAT IS MICROPROPAGATION ?A whole plant can be regenerated from a small tissue orplant cells in a suitable culture medium under controlledenvironment. The plantlets so produced are called tissue-culture raised plants. 7
  • 8. WHY DO MICROPROPAGATION ? A single explant can be multiplied into several thousand plants in less than a year – this allows fast commercial propagation of new cultivars. Once established, a plant tissue culture line can give a continuous supply of young plants throughout the year. In plants prone to virus diseases, virus free explants (new meristem tissue is usually virus free) can be cultivated to provide virus free plants. 8
  • 9. CONT.…  Plant ‘tissue banks’ can be frozen, then regenerated through tissue culture.  Plant cultures in approved media are easier to export than are soil-grown plants, as they are pathogen free and take up little space (most current plant export is now done in this manner). 9
  • 10. LIST OF BANANA AND PLANTAIN DISEASESBacterial diseasesBacterial wilt Pseudomonas solanacearumFinger tip rot (gumming) Pseudomonas sppRhizome rot Erwinia carotovoraFungal diseasesAnthracnose Colletotrichum musaeBlack cross Phyllachora musicolaBlack leaf streak Mycosphaerella fijiensisBlack root rot Rosellinia bunodesCrown rot Verticillium theobromae Source:- Wikipedia, the free encyclopedia 10
  • 11. ADVANTAGES OF MICROPROPAGATION1.Rapid multiplication2.Requirement of limited mother stock3.Product uniformity4.Season independent production5.Agronomic advantages6.Plant exchange7.High returns 11
  • 12. Source : Cadila pharmaceuticals limited 12
  • 13. STAGE 0 : SELECTION AND MAINTENANCE OF STOCK PLANTS FOR CULTURE INITIATION EXPLANT SHOOT TIPS FROM YOUNG SUCKER APICAL MERISTEM (1-2 Cm3) SURFACED STERILIZATION 13
  • 14. STAGE- I : INITIATION AND ESTABLISHMENT OF ASEPTIC CULTURESource: Jain Irrigation Systems Ltd 14
  • 15. INITIATION 15
  • 16. STAGE –II : MULTIPLICATION OF SHOOT 16
  • 17. STAGE -III: ROOTING 17
  • 18. STAGE –IV: ACCLIMATIZATION / HARDENING 18
  • 19. SECONDARY HARDENINGPlants transferred to nursery bagsKept for 6 to 8 weeks under 50% shade Regular foliar spraysVariation if observed is discardedPlant ready for sale (1 feet height) 19
  • 20. FACTORS AFFECTING PLANT MICROPROPAGATION 1. Growth Media 2. Environmental Factors 3. Explants Source 4. Genetics 20
  • 21. BANANA PLANTS READY FOR SALE 21
  • 22. IDEAL TISSUE CULTURE RAISED PLANTAn ideal tissue culture raised plant should: Be 30 cm in height and have a pseudostem circumference of 5.0-6.0 cm after 60 days of total hardening. Have 4-5 photosynthetically active leaves and inter-foliar space must be not less than 5.0 cm. Have approximately 25-30 more than 15 cm active roots at the end of secondary hardening. 22
  • 23. CONT…. Be free from any visual symptoms of leaf spot, pseudostem rot and physical deformations; Be free from root pathogens like Erwinia, nematode lesions and root knots. Random checking of roots is essential to ensure health of plantation. 23
  • 24. PROJECTED MULTIPLICATION RATE OF BANANA UNDER OF TISSUE CULTURE FROM A SINGLE EXPLANTParticulars Stage Generation Duration (days) No. of plants Initiation 25 1 Subculture 1st 50 3 stages 2nd 75-80 12 3rd 100-110 48 4th 125-130 192 5th 175-180 760 6th 200-210 3,040 7th 225-230 12,160 Rooting 255-260 11-12000 Primary 270-280 11,500-11,000* hardening Secondary 310-320 10,000-10,500* hardeningShukla S. 2008 24
  • 25. Hand FingerSource: Jain Irrigation Systems Ltd 25
  • 26. SUCKERS TISSUE CULTURESource: Jain Irrigation 26
  • 27. COMPARATIVE INCOME FROM TISSUE-CULTURE AND SUCKER-PROPAGATED BANANASSl. Parameters TC Sucker ComparisonNo propagated propagated percentage bananas bananas1 Mean yield (bunches/ha) 2,663 2,416 -2 Mean price received (/bunch) 94.47 76.42 -3 Value of main product (/ha) 2,51,573 184,630 -4 Value of by-product (/ha) 1729 2,518 -5 Gross income (/ha) 2,53,302 187,149 26%6 Total expenses (/ha) 1,41,040 108,294 23%7 Net income (/ha) 1,12,262 78,855 30%8 Cost of production per bunch 52.31 43.78 -9 Net income per bunch 42.16 32.64 -Source: Hanumantharya et al. (2009) 27
  • 28. VARIETIES GROWN IN INDIAState Varieties grownMaharashtra Grand naine, safed velchi, (AB), Dwarf Cavedish, Shindhuri, Ardhapuri, Lalvelchi (Mysore), Rajeli (Plantain).Gujarat Grand naine, Dwarf Cavedish, Lactan, Harichal, Gandevi Selection.Tamil nadu Grand naine, matti, sevvazhai (red banana), ney poovan, robusta poovan (mysore), sthali (silk), virupakshi (pome), pachanadan, nedran, sakkai.Andhra Pradesh Grand naine, Dwarf Cavedish, robusta.Assam Jahaji, borjahaji, manjahaji, robusta, honda, chenichampa, alpan, malbhoj (silk), jatikol (mysore) manohar, bhimkol.Bihar Dwarf Cavedish, chinai, chenichampa, alpan (mysore), malbhoj (silk), gauria .Karnataka Grand naine, Dwarf Cavedish, poovan (mysore), robusta , elakki bale.Kerala Njali poovan, red banana, nendran (plantain), palayankondan (mysore) poovan silk, monthan.West bengal Amrit sagar, giant governor, lacton, mortman (silk), champa 28
  • 29. PRODUCTION CAPACITY OF TISSUE CULTURE COMPANIES IN INDIAState Company Name Production Capacity (millions per annum)Gujarat Cadila Pharmaceuticals Ltd. 10.00 Shaili Biotech (P) Ltd. 6.00 Sarjan Biotech Pvt. Ltd. 5.00 Arcadia Agro 3.60Maharashtra Jain Irrigation Systems Ltd. 60.00 Rise N Shine Biotech Pvt. Ltd. 15.00 Gargi Biotek Pvt. Ltd. 15.00 H.U Gugle Agro Biotech Co. 5.00 Seema Biotech 2.00Andhra Pradesh Godrej Agrovet Ltd. 5.00 Anantha Biotechnologies 3.50 A.G. Bioteck Laboratories (India) 3.00 Ltd. Agri Vitro Tech Laboratories 3.00 Brookfileds Biotech Pvt. Ltd. 1.50 29
  • 30. CONT…. Tamil Nadu SPIC Agro Biotech Centre 7.00 Growmore Bioteh Ltd. 10.00 Uttarakhand Aryave Biotech Pvt. Ltd. 2.00 Uttar Pradesh Tissue Culture Facility, Biotech 0.60 Park Haryana Sheel Biotech Ltd. 6.00 West Bengal Boinchi Bioplants Pvt. Ltd. 2.00 Vedic Synergy Biotech. Ltd. 7.00 Elegant Flower Company Pvt. 1.50 Ltd. Karnataka Lakshmi Biotech 3.00 Green Earth Biotech. Ltd. 3.00 Lab and Biotech Pvt. Ltd. 3.00 Shri Ramco Biotech 5.0 K.F. Biotech Pvt Ltd. 3.00 Chattisgarh Aditya Biotech Lab & Research 1.00 Pvt Ltd. Devleela Biotechs 1.00 30Shukla S. 2008
  • 31. MARKET PROJECTIONS FOR TISSUE CULTURE BANANA PLANTS IN INDIA Year 2004-05 2007-08 2010-11 (million) (million) (million)Anticipated Volume 27.50 64.00 80.00 Value 330.00 768.00 960.00 @`Rs. 12/plant @`Rs. 12/plant @`Rs. 12/plant Actual Volume 30.00 51.00 Value 2750 765.00 @`Rs. 15/plant @`Rs. 15/plantSources : Survey report of DBT 2005 31
  • 32. AREA UNDER TISSUE CULTURE AND SUCKER PROPAGATED BANANAS Area(Mha) Percentage Tissue culture 0.053 7 Sucker propagated 0.709 93 Total 0.770 100source : www.apcoab.org. 32
  • 33. ESTABLISHMENT OF COMMERCIAL PLANT TISSUE CULTURE UNIT  COMPONENTS: 1) STORAGE ROOM FOR CHEMICALS. 2) MEDIA PREPARATION ROOM. 3) WASHING ROOM. 4) INOCULATION ROOM. 5) GROWTH ROOM. 6) GREEN HOUSE. 33
  • 34. ECONOMICS OF STARTING PTC BUSINESS WITH THE MINIMAL INVESTMENT Capacity 3 lakh plantlets per annumCAPITAL (Rs. lakhs)INVESTMENTFixed assets 10.10Working capital(3 3.63month)Total 13.73 34 34
  • 35. D. MEANS OF FINANCEDebt 10.25Equity 3.63Total 13.73Debt : Equity 3:2Rate of interest on loans 17% 35
  • 36. CONT….(I) Cost of Production (per annum) (Rs. lakhs)Recurring cost 14.52Depreciation (@10%) 0.80Interest (@17%) 1.31Total 16.63 36
  • 37. CONT….(II) TurnoverTotal plantlets 3 lakhsSelling price Rs. 8 per plantTotal turnover Rs. 24 lakhsNet profit Rs. 7.37 lakhs% Profit on sales 30%IRR 19%Return on investment 56%Source : DBT Government of India 37
  • 38. NEED FOR CERTIFICATION OF MICROPROPAGATED RAISED PLANTS  Department of Biotechnology (DBT), Government of India has established National Certification System for Tissue Culture Raised Plants (NCS TCP).  Regulate genetic fidelity as authorized vide the Gazette of India Notification dated 10th March 2006 of Ministry of Agriculture under section 8 of the Seeds Act, -1966. 38
  • 39. ACCREDITATION OF LABORATORIES AND CERTIFICATION OF PRODUCTION FACILITY TC CertificationApplication Authority Application Tissue CultureTest Accreditation/Certification ProductionLaboratory Unit Facility Accreditation/Certification Site visit Panel Site visit Evaluation Report Accreditation/Certification Unit Certified TC Production Facility Accreditation /Certificates 39
  • 40. PROCESS FOR CERTIFICATION TISSUE CULTURE OF RAISED PLANTS Certified TC Production Facility Application Site visit Accredited Test Laboratory Testing of Samples Test Report Positive Certificate of Quality (CQ-TCP) 40
  • 41. FEE STRUCTURE FOR CERTIFICATION OF TISSUE CULTURE PRODUCTION FACILITIES (FIGURES IN RS.) Small-scale Medium-scale Large-scale companies companies companies (Up to 1 million (1 to 3 million (>3 million plantlets/annum) plantlets/annum) plantlets/annu m)Registration fee 1000 1000 1000Inspection and 1000 3000 5000report preparationProcessing and 5000 5000 5000CertificationPERIOD OF VALIDITY OF CERTIFICATION IS TWO YEARS 41
  • 42. LABELING BANANA-TISSUE CULTURE (BTC) BTC shall be supplied in containers. A cloth-lined label of 12cm x 6 cmcontaining following information shall be affixed on the containerCrop Banana Label No. :VarietyClass of material : BTCLot No. :BatchAccredited test laboratoryand certification reference noteDate of certificationProduction Agency :(Name and address) Source:-Biotech consortium India Ltd.New delhi 42
  • 43. GOVERNMENT SCHEMES AND INCENTIVES Agricultural and Processed food products Export Development Authority (APEDA). National Horticulture Board (NHB). Department of Biotechnology (DBT). Small Farmers Agri business Consortium (SFAC). State Level Incentives. Financial Assistance by Banks. 43
  • 44. AGRICULTURAL AND PROCESSED FOOD PRODUCTS EXPORT DEVELOPMENT AUTHORITY (APEDA)  50% subsidy is given for the development of infrastructure.  Financial assistance is also given for strengthening quality control facilities and implementation of ISO 9000. 44
  • 45. NATIONAL HORTICULTURE BOARD (NHB) For setting up of a new tissue culture lab there is a provision for project cost with a maximum limit of Rs. 25 lakh per project. NHB also has a scheme for providing subsidy for cultivation under controlled climate condition in poly houses, green houses, net houses, etc. 45
  • 46. DEPARTMENT OF BIOTECHNOLOGY (DBT) DBT has supported 150 projects so far for development of micropropagation related protocols for about 50plant species. DBT established National Certification System for Tissue Culture Raised Plants vide the Gazette of India Notification dated 10th March 2006 of Ministry of Agriculture under section 8 of the Seeds Act, 1966 46
  • 47. SMALL FARMERS AGRI BUSINESS CONSORTIUM (SFAC)  SFAC under the Ministry of Agriculture give soft loans up to 50 lakhs for setting up of small tissue culture labs by cooperative societies formed by small scale farmers. 47
  • 48. STATE LEVEL INCENTIVES The states of Karnataka, Gujarat, Maharashtra and Andhra Pradesh are giving financial assistance of 20% on investments for setting tissue culture labs under the new agro industrial policy. 48
  • 49. FINANCIAL ASSISTANCE BY BANKS Some nationalized banks like Canara Bank has opened a special cell for financing high tech agriculture projects. National Bank for Agriculture and Rural Development (NABARD) under its refinancing scheme has supported some 30 projects with 32% subsidy on investment 49
  • 50. MICROPROPAGATION OF BANANA SWOT ANALYSIS STRENGTH:  Agro climetic conditoin is suitable for tissue culture banana Cultivation  Bunches are uniform, big finger size and average 140-150 fingers per bunch.  Financing institution are also interested to finance of commercial production of banana tissue culture. 50
  • 51. WEAKNESS  It requires more investment in initial stage.  It is relatively sophisticated technology than suckers. and need to be well equipped with knowledge and skills. 51
  • 52. OPPORTUNITY  Heavy market demand with good price.  The enterpreneur could get subsidy and credit facilities . 52
  • 53. THREATS  There will be heavy damage to the plant if there is cyclone and hailstorm.  Complete watch and ward is necessary for discriminates for storing bunches.  Heavy winter during bunch initiation period reduces the bunch size and occurrence of throat choke disease. 53
  • 54. CONCLUSION India is being looked upon by the world as the main technology base for production and supply of economically important plant varieties. The plant tissue culture technology has been very successful as an industry and has greatly contributed to successful agriculture and Indian farmers are becoming aware about the propagation of tissue culture because of high yielding productivity. But, only 7 percent area of banana in India is under tissue culture plantation. New entrepreneurs having sound scope in production of banana micropropagated plantlets, will get 7.37 lakh from 3 lakh plantlets per annum. 54
  • 55. 55

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