39 Matthew Blair Tli Objective3 Phase Ii Work Plan
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39 Matthew Blair Tli Objective3 Phase Ii Work Plan

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39 Matthew Blair Tli Objective3 Phase Ii Work Plan 39 Matthew Blair Tli Objective3 Phase Ii Work Plan Presentation Transcript

  • TL1 proposal: common bean TL2 annual Meeting, Bamako Mali – Nov. 2009 (Objective 3 – Tropical Legumes I)
  • Objective 3 Common bean – Major Outcome flows to TL-1 Phase II Germplasm Genomic Marker Population Activity Screening Resources Associations development 2007 Germplasm phenotyped SSR platform (1500) from Tools (604) drought tolerance EST, BAC, etc., and genotyping sets MAS crosses (CBB, Adv Backross 1st gen. SNP platform (768) BCMV, Bruchid) populations created (5) Germplasm genotyped Intra-genepool Elite x QTL populations Phase I phenotyped (deep Elite Crosses (40) Operation Mapped markers / rooting and carbohyd. Sources of drought Physical map translocation) Inter-genepool lines tolerance (DAB and CBIB lines) Advanced lines Diversity Sets: and segregating populations Early generation Ethiopia, Kenya, QTL for deep rooting populations and ECABREN / and for carbohydrate advanced lines SABRN and translocation Reference Coll. Outcome 2010 Phase II Deep rooting sources SNP confirmation Marker-assisted Adv. Backcross and backcrossing to MARS breeding For Intra-genepool develop improved populations for populations local varieties Andean drought tolerance 2014
  • Act. 1: Evaluate rooting depth of parental sources •  Extending work on reference and regional collections (G. Makunde, Ph.D. thesis) View slide
  • Act. 2: Validate SNPs for inter- and intra- gene pool crosses Mesoamericans •  For 50 donor and recipient genotypes •  For introgression to Andeans •  For use in MARS Andeans View slide
  • Act. 3: Deploy insect and disease resistance genes •  Confirm MAS in advanced lines –  BCMNV –  Storage insects –  Bacterial Blight •  RGA for Bacterial blight
  • Act. 4: Deploy drought tolerance traits from inter-gene pool o o o o crosses in Andean o gene pool Pod Pod Partitioning Harvest Index Index •  Characterize traits in 40 parentals •  Develop & test visual scales •  QTL for Pod Harvest Index & deep rooting •  Test 60 Andeans for heat tolerance
  • Act. 5: Pyramid drought tolerance traits through MARS and Advanced Backcross in Andean gene pool P1 P2 •  Genotype 2 AB populations F1 •  Genotype & F1-BC1 phenotype 4 RIL populations for MARS F1-BC2 •  3 more populations for MARS X •  Pyramiding from AB and RILs Advanced Backcross Lines
  • Advanced
Backcross
Scheme
 Andean Red Mottled 2 AB-QTL Microsatellites and SNPs Cultivars Populations CAL96 (Uga) selected CAL143 (Mwi) Phenotype Genotype Genetic Map QTL identification Mesoamerican Drought Tolerance Sources SER16 Line distribution SER48
  • Marker
Assisted
Recurrent
Selection
Scheme
 DNA extraction Genotyping Lines Derived and QTL analysis Recombination Cycles F3.5: Drought Multi-location trials option Phenotyping for QTL analysis Double & Multiple F3.4: Families Lowland site crosses F1: Simple cross Cycle 1 Drought tol. X And. commercial starts F3: Plant select. Low P + MAS for R gene Highland site F1 to F2 F2: Drought Single Plant Selection Lowland site
  • Act. 6: Training of students in drought tolerance and MAS •  Training students in MARS •  Short course in CIAT Act. 7: Data storage and management
  • Proposed Act. 8: Testing the out-sourcing of marker analysis with partners •  Samples currently cost $8 in Africa (paid by special project using unique method) •  Outsourcing would cost $2-3 •  Accuracy of markers would be verified –  Bacterial blight –  Storage insects