1. TL1 proposal: common bean
TL2 annual Meeting, Bamako Mali – Nov. 2009
(Objective 3 – Tropical Legumes I)

2. Objective 3 Common bean – Major Outcome flows to TL-1 Phase II
Germplasm Genomic Marker Population Activity
Screening Resources Associations development
2007
Germplasm phenotyped SSR platform (1500) from Tools
(604) drought tolerance EST, BAC, etc., and
genotyping sets MAS crosses (CBB, Adv Backross
1st gen. SNP platform (768) BCMV, Bruchid) populations created (5)
Germplasm genotyped Intra-genepool Elite x
QTL populations
Phase I
phenotyped (deep Elite Crosses (40) Operation
Mapped markers / rooting and carbohyd.
Sources of drought Physical map translocation) Inter-genepool lines
tolerance (DAB and CBIB lines)
Advanced lines
Diversity Sets: and segregating
populations Early generation
Ethiopia, Kenya, QTL for deep rooting populations and
ECABREN / and for carbohydrate advanced lines
SABRN and translocation
Reference Coll. Outcome
2010
Phase II
Deep rooting sources SNP confirmation Marker-assisted Adv. Backcross and
backcrossing to MARS breeding
For Intra-genepool
develop improved populations for
populations
local varieties Andean drought
tolerance
2014

3. Act. 1: Evaluate rooting depth of
parental sources
• Extending work
on reference and
regional
collections
(G. Makunde,
Ph.D. thesis)

4. Act. 2: Validate SNPs for inter- and intra-
gene pool crosses
Mesoamericans
• For 50 donor and
recipient genotypes
• For introgression to
Andeans
• For use in MARS
Andeans

5. Act. 3: Deploy insect and disease
resistance genes
• Confirm MAS in
advanced lines
– BCMNV
– Storage insects
– Bacterial Blight
• RGA for Bacterial
blight

6. Act. 4: Deploy drought
tolerance traits from
inter-gene pool
o
o
o
o
crosses in Andean
o
gene pool Pod Pod
Partitioning Harvest
Index Index
• Characterize traits in 40 parentals
• Develop & test visual scales
• QTL for Pod Harvest Index & deep
rooting
• Test 60 Andeans for heat tolerance

7. Act. 5: Pyramid drought tolerance traits
through MARS and Advanced Backcross
in Andean gene pool
P1 P2
• Genotype 2 AB
populations F1
• Genotype & F1-BC1
phenotype 4 RIL
populations for MARS
F1-BC2
• 3 more populations for
MARS X
• Pyramiding from AB
and RILs
Advanced Backcross Lines

8. Advanced Backcross Scheme
Andean
Red Mottled 2 AB-QTL Microsatellites and SNPs
Cultivars Populations
CAL96 (Uga) selected
CAL143 (Mwi)
Phenotype
Genotype
Genetic Map
QTL identification
Mesoamerican
Drought Tolerance
Sources
SER16 Line distribution
SER48

9. Marker Assisted Recurrent Selection Scheme
DNA extraction
Genotyping
Lines Derived and QTL analysis
Recombination Cycles
F3.5: Drought
Multi-location trials option
Phenotyping for QTL analysis
Double & Multiple F3.4: Families
Lowland site
crosses
F1: Simple cross Cycle 1
Drought tol. X And. commercial starts F3: Plant select.
Low P + MAS for R gene
Highland site
F1 to F2 F2: Drought
Single Plant Selection
Lowland site

10. Act. 6: Training of students in
drought tolerance and MAS
• Training students in MARS
• Short course in CIAT
Act. 7: Data storage and
management

11. Proposed Act. 8: Testing the out-sourcing of
marker analysis with partners
• Samples currently cost $8 in Africa (paid by
special project using unique method)
• Outsourcing would cost $2-3
• Accuracy of markers would be verified
– Bacterial blight
– Storage insects