Nutriplant Organics Product Training Cytozyme Laboratories

Nutriplant Organics Product Training

Cytozyme Laboratories An American company founded in 1975 Makes only biologically/organic products

An American company founded in 1975

Makes only biologically/organic products

Brought you Nutriplant AG Nutriplant SD Nutriplant SL Apsa80 Cytozyme Laboratories

Brought you

Nutriplant AG

Nutriplant SD

Nutriplant SL

Apsa80

20 processing steps 10 points of quality control Crop+Plus Nutriplant AG Manufacturing and QC Shipping Receiving Weighing Stabilization Screening Packaging Mixing Natural Ligands Seaweed Extracts Minerals Batching Chelation

N UTRIPLANT ® AG Product Technology N UTRIPLANT ® technology utilizes the latest scientific discoveries in biochemistry, agronomy, and chemical engineering.

NUTRIPLANT ® AG Superior Quality N UTRIPLANT ® is made from the best quality raw materials, with NO processed animal parts, NO waste and NO GMO (genetically modified organisms).

N UTRIPLANT ® AG Superior Quality Each batch of product undergoes rigorous quality control procedures.

N UTRIPLANT ® AG Superior Quality The products are analyzed for physical properties and chemical composition.

Product performance is verified by a unique bioassay of biological activity. N UTRIPLANT ® AG Superior Quality

N UTRIPLANT ® Root Promoter Available in 2 forms: Powder = Nutriplant SD for dry seeds Liquid = Nutriplant SL for soaking seeds and for all transplants

Available in 2 forms:

Powder = Nutriplant SD for dry seeds

Liquid = Nutriplant SL for soaking seeds and for all transplants

Laboratory results Corn not treated Corn treated Seed Treatment increases the root hairs, crucial for water and nutrient uptake .

N UTRIPLANT ® SD on Corn

What is Nutriplant AG? Naturally derived (from plants and minerals) Foliar (goes on leaves) Biostimulant (stimulates photosynthesis and plant growth) Contains complete balanced nutrition (macro and micro elements, in small quantities)

Naturally derived (from plants and minerals)

Foliar (goes on leaves)

Biostimulant (stimulates photosynthesis and plant growth)

Contains complete balanced nutrition (macro and micro elements, in small quantities)

N utriPlus™ NPK N utriPlus™ Potassium N utriPlus™ Zinc Nutriplant New 2009 Agri Products

N utriPlus™ NPK

N utriPlus™ Potassium

N utriPlus™ Zinc

Organically chelated foliar fertilizers To prevent and overcome deficiencies Product Description NutriPlus

Organically chelated foliar fertilizers

To prevent and overcome deficiencies

Amino acids, organic acids, and carbohydrates (to protect then minerals and make them more effective) Small molecular weight (95-800) to enter the leaves and plant cells faster Fast acting, 100% available to plants) NutriPlus products are chelated/complexed with nature’s own chelates: NutriPlus

Amino acids, organic acids, and carbohydrates (to protect then minerals and make them more effective)

Small molecular weight (95-800) to enter the leaves and plant cells faster

Fast acting, 100% available to plants)

How Are Chelates Formed? Natal Chelate= a mineral surrounded amino acids Mineral

NutriPlus products are chelated/complexed with nature’s own chelates: This means the minerals are protected and more effective. NutriPlus Metal Amino acids, carboxylic acids , etc. Chelate

Close Up Large molecules can only enter via stomata Stomata are open only part of the time Natural amino and carboxylic acids can enter via stomata and directly through leaf epidermis

Large molecules can only enter via stomata

Stomata are open only part of the time

Natural amino and carboxylic acids can enter via stomata and directly through leaf epidermis

Soil fertilization (apply to soil) Foliar fertilization (apply to leaves) How to Prevent or Overcome Nutrient Deficiencies?

Soil fertilization (apply to soil)

Foliar fertilization (apply to leaves)

Advantage of Foliar Fertilization over Soil Fertilization Soil Application Foliar Application Soil Application Ties up Slow acting Less efficient Leaches Foliar Application Faster uptake (within hours) No tie up by soil More efficient (3-100 times) No leaching Activate plant metabolism stimulating photosynthesis, uptake of water and nutrients Foliar applications are faster acting and more efficient!

Soil Application

Ties up

Slow acting

Less efficient

Leaches

Foliar Application

Faster uptake (within hours)

No tie up by soil

More efficient (3-100 times)

No leaching

Activate plant metabolism stimulating photosynthesis, uptake of water and nutrients

Importance of Balanced Nutrition The limiting factor for holding capacity of a barrel is the shortest stave. The limiting factor for growth is the nutrient in shortest supply. A short supply of nutrients reduces yields, causing reduced profits. Iron Zinc Potassium Nitrogen Copper Boron Calcium Iron Zinc Potassium Nitrogen Copper Boron Calcium

Nutrient Deficiencies By the time deficiencies are visible, yields have been reduced! Prevent deficiencies with a regular maintenance program, or with frequent foliar analyses followed by application of the appropriate nutrient(s). Visible deficiencies Hidden deficiencies Yields Genetic Potential Nutrient deficiency

Examples of Visible Deficiencies Iron Potassium Zinc By the time deficiencies are visible, yields have been reduced!

Magnesium and Zinc Deficiencies in Banana Leaves and Fruits Zinc deficiency Magnesium deficiency Double - Zinc deficiency Quadruples - Zinc deficiency

When soil or climatic conditions are less than ideal, even if soil fertilization is optimal: Too hot, too dry, too wet, poor soils, etc. During stages of rapid plant growth During critical stages of development, such as flowering, fruit set, and fruit maturity when deficiencies will cause yield reductions When Are Foliar Applications Needed? NutriPlus

When soil or climatic conditions are less than ideal, even if soil fertilization is optimal:

Too hot, too dry, too wet, poor soils, etc.

During stages of rapid plant growth

During critical stages of development, such as flowering, fruit set, and fruit maturity when deficiencies will cause yield reductions

Salt solutions (such as zinc sulfate) Synthetic chelates (such as EDTA zinc) Natural chelates Choices Available to Farmers NutriPlus™

Salt solutions (such as zinc sulfate)

Synthetic chelates (such as EDTA zinc)

Natural chelates

Crystal Deposits of Mineral Salts like Zinc Sulfate Ammonium sulfate, etc on Leaf Surface Dr. Zeev Wiesman. R & D Lab of Bio-Industries Upgrading, The Institutes For Applied Research. Ben Gurion University of the Negev, Beer Sheva.

Effects of NutriPlus on pH of Water NutriPlus decreases the pH which increases rate of absorption and translocation .

Why NutriPlus N PK? To supplement soil fertilizer Overcome and prevent deficiencies Easier to handle than single applications Faster acting than soil fertilizers 4-18-18 (% wt/wt) NutriPlus™ NPK N, P, K

Why NutriPlus N PK?

To supplement soil fertilizer

Overcome and prevent deficiencies

Easier to handle than single applications

Faster acting than soil fertilizers

Economic benefits of NutriPlus™ NPK: Improved flowering and fruit set Improved quality Increased yields Increased profits! 15% yield increase! 41% yield increase! NutriPlus™ NPK

Improved flowering and fruit set

Improved quality

Increased yields

Functions of potassium include: Photosynthesis Plant water relations Lignin and cellulose development Protein development (25%) NutriPlus™ Potassium P

Functions of potassium include:

Photosynthesis

Plant water relations

Lignin and cellulose development

Protein development

Economic benefits of NutriPlus™ Potassium: Improved crop quality Stronger stems and stalks (less lodging Increased yields Increased profits! 33% yield increase! 187% yield increase! NutriPlus™ Potassium

Improved crop quality

Stronger stems and stalks (less lodging

Increased yields

Functions of zinc include: Production of natural hormones Protein and carbohydrate synthesis Activation of enzymes Stabilization of membranes Antioxidant (detoxification of free radicals) (8.1%) NutriPlus™ Zinc Zn

Functions of zinc include:

Production of natural hormones

Protein and carbohydrate synthesis

Activation of enzymes

Stabilization of membranes

Antioxidant (detoxification of free radicals)

Economic benefits of NutriPlus™ Zinc: Better root development Increased leaf size Increased grain formation More uniform maturity Increased yields Improved fruit quality Increased profits! 57% yield increase! 67% yield increase! NutriPlus™ Zinc

Better root development

Increased leaf size

Increased grain formation

More uniform maturity

Increased yields

Improved fruit quality

Increases crop yield and quality through these modes of action: Increased chlorophyll content and photosynthesis Increased nutrient uptake through root Antioxidant activity to reduce abiotic stress Elicitor activity to reduce biotic stress How Do NUTRI Products Work?

Increases crop yield and quality through these modes of action:

Increased chlorophyll content and photosynthesis

Increased nutrient uptake through root

Antioxidant activity to reduce abiotic stress

Elicitor activity to reduce biotic stress

Mode of Action I N UTRIPLANT AG Enhances Two Critical Processes in Crop Physiology 1. Photosynthesis 2. Nutrient uptake through roots

N UTRIPLANT AG Increases Chlorophyll Development Photosynthesis is how the plant traps energy from the sun.

N UTRIPLANT AG Increases Chlorophyll Development

Sources: Cotton and rice data from University of Arkansas. Tea data from UPASI Tea Research Foundation N UTRIPLANT AG Enhances Photosynthesis Crop Photosynthesis Increase (% over Control) Yield Increase (% over Control) Cotton 24% 22% Rice 34% 16% Tea 15% 8%

-5 0 5 10 15 20 25 30 35 40 DAYS AFTER APPLICATION 1,3 1,2 1,1 1,0 0,9 0,8 PHI E 0 RELATIVO = ET / ABS ( MEDIDO POR FLUORESCENCIA DE LA CLOROFILA ) CL - 143 HI1- 3305 CONTROL Effects of Two Different P roducts on Photosynthetic Activity W hen Applied to Grape Vines Louvieaux ( 2.004 ) 1º 2º 3º

Mode of Action II Increased Absorption of Nutrients by Roots

Plants with foliar applications of N UTRIPLANT AG absorbed 56% more Mn, 200% more Fe and Zn, and 370% more Cu N UTRIPLANT AG Increases Absorption of Nutrients by Roots

Even with optimal photosynthesis and nutrient uptake, crops do not reach their full genetic potential due to STRESS.

Crops produce only 24% of their yield potential. 65% of the yield is lost to abiotic stress 11% to biotic stress 1 Mean of 6 crops: barley, maize, potatoes, soybeans, sugar beets, wheat Bray, Bailey-Serres and Weretilnyk . 2000. Responses to abiotic stresses. In: W Gruissem, B. Biology of Plants. American Society of Plant Physiologists, Rockville, MD, pp 1158–1249. Mode of Action III Abiotic Stress (Extreme temperatures, UV radiation, drought, salinity, etc.)

Crops produce only 24% of their yield potential.

65% of the yield is lost to abiotic stress

11% to biotic stress

Abiotic stress: High temperatures Excessive UV radiation Excessive light Ozone Drought Root water logging/axphixiation Low temperatures Freezing Wind Nutrient deficiency Salinity Too much shade / light deficiency Heavy metals

Abiotic stress:

High temperatures

Excessive UV radiation

Excessive light

Ozone

Drought

Root water logging/axphixiation

Low temperatures

Freezing

Wind

Nutrient deficiency

Salinity

Too much shade / light deficiency

Heavy metals

Abiotic Stress Includes Photooxidation (Damage Caused by ‘Free radical Oxygen’) Occurs in crops when there is excess light Photosynthesis is reduced, resulting in reduced productivity Leaves may turn yellow, and drop early

Occurs in crops when there is excess light

Photosynthesis is reduced, resulting in reduced productivity

Leaves may turn yellow, and drop early

Increase in UV - 305 nm Radiation at Between 1992 and 1998 * Average daily values are grouped into monthly averages to calculate a mean value for each month of the year, which is substrated from each monthly mean. These values are the difference between ‘Zero” (equivalent To each montly mean. The regression has a confidence interval of 95 % Source: Cabrera. 2005 At Santiago, Chile.

Net Photosynthesis ( µ mol CO 2 / m 2 / sec. ) Light intensity ( µ mol of photons / m 2 / sec ) 0 2.000 1.000 500 1.500 Photo-thermic Stress 2.500 10 9 8 7 6 5 4 3 2 1 0

Stomates Close An Example of Stress: Response to Excess Solar Radiation H 2 O H 2 O H 2 O ABA

Photooxidative Damage !!

What happens when fruits get hot?

Aloni et al ( 2005 ) Leaves cannot cool themselves like leaves do, so they are always hotter than leaves.

Glenn ( 2.006 ) 52 ± 1 ° C 46 – 49 °C

Heat stress/sunburn in fruit

Schrader et al ( 2.003 ) Stress Induced Disorder: Effect on Apple Fruit Quality. WSU Physiological Disorders induced by Heat Stress Bitter Pit Lenticelosis Splitting Water core Sunscald

CH 2 =CH 2 H 2 O 2 . O 2 - Fruit affected by Abiotic stress

The oxidative process begins at the memrane level.

Growth Maturation Aging Deteriratin Oxidation !

Reactive Species ( Oxidants ) Free radicals Non- RADICALS . O 2 - H 2 O 2 OH - HOCl NO . O 3 LOO . SUPEROXIDE E( Zn -SOD ; Mn -SOD ) CATALASES ASCORBIC ACID GLUTATHION TOCOFEROL CAROTENOIDS FLAVONOIDS ANTIOXIDANTS Oxidative stress is a biochemical state of the cell or tissue which can occur over a short or long period, where the generation of chemical oxidative species exceeds the capacity to produce antioxidants. M c Kersie, 1996

SUPEROXIDE DISMUTASES ( Zn -SOD ; Mn -SOD ) CATALASES. ASCORBIC ACID GLUTATHION TOCOFEROLS CAROTENOIDS FLAVONOIDS. Reactive Species of Oxigen ( OXIDANTS ) Free Radicals NON- RADICALS . O 2 - H 2 O 2 OH - HOCl NO . O 3 LOO . ANTI - OXIDANTS

Nutri products have Strong Antioxidant Activity NutriPlant AG has 10 X more! Product Name Antioxidant Activity (TE/100 g sample) NUTRIPLANT AG™ 10,133 NutriPlus Calcium™ 2,933 Biozyme™ 1,422 Kendal™ 703 Auxym™ 587 Stimulate™ 203 BM86™ 8 Kelpak™ 0 Cytogrower™ 0

Review

Review Nutriplant AG Foliar Biostimulant, increasing photosynthesis for more plant energy Stimulates plant growth Reduces effects of environmental stress Complete, balanced nutrition

Nutriplant AG

Foliar Biostimulant, increasing photosynthesis

for more plant energy

Stimulates plant growth

Reduces effects of environmental stress

Complete, balanced nutrition

Review NutriPlus NPK Foliar fertilizer Supplies Nitrogen, Phosphorus and Potassium directly to the leaves To prevent and overcome deficiencies Faster than NPK applied to the soil More efficient (none is lost in the soil)

NutriPlus NPK

Foliar fertilizer

Supplies Nitrogen, Phosphorus and Potassium directly to the leaves

To prevent and overcome deficiencies

Faster than NPK applied to the soil

More efficient (none is lost in the soil)

Review NutriPlus Potassium Foliar fertilizer Supplies Potassium directly to the leaves To prevent and overcome deficiencies of potassium Faster than applying Potassium to the soil For efficient than (none is lost in the soil)

NutriPlus Potassium

Foliar fertilizer

Supplies Potassium directly to the leaves

To prevent and overcome deficiencies of potassium

Faster than applying Potassium to the soil

For efficient than (none is lost in the soil)

Review NutriPlus Zinc Foliar fertilizer Supplies zinc directly to the leaves To prevent and overcome deficiencies of zinc Faster than putting zinc in the soil More efficient (none is lost in soil)

NutriPlus Zinc

Foliar fertilizer

Supplies zinc directly to the leaves

To prevent and overcome deficiencies of zinc

Faster than putting zinc in the soil

More efficient (none is lost in soil)

Growth promotors Biostimulants Antioxidants Biologically active nutrients, chelated/complexed with natural organic ligands Nutri Products Combining the Power of

Growth promotors

Biostimulants

Antioxidants

Biologically active nutrients, chelated/complexed with natural organic ligands

Increases crop yield and quality through these modes of action: Increased chlorophyll content and photosynthesis Increased nutrient uptake through root Antioxidant activity to reduce abiotic stress Elicitor activity to reduce biotic stress 4 Modes of Action

Increases crop yield and quality through these modes of action:

Increased chlorophyll content and photosynthesis

Increased nutrient uptake through root

Antioxidant activity to reduce abiotic stress

Elicitor activity to reduce biotic stress

Fertilizer only Do not stimulate photosynthesis Do not have combat effects of stress Do not have antioxidant activity Other Foliar Fertilizers

Fertilizer only

Do not stimulate photosynthesis

Do not have combat effects of stress

Do not have antioxidant activity

Salamat Po!