EURL CPS, Lm, MMP                                                                                                         ...
French Agency for Food,               Environmental and Occupational                        Health SafetyEURL CPS, Lm, MMP...
Relations with the government and                               ParliamentMinistry for ecology                            ...
Missions              Assess nutritional and health            Recommend public health                   risks and benefit...
ANSES in brief 1350 staff 800 external experts solicited (20 Expert Committees,  working groups) Annual budget: 140 mil...
Pôle HQSA                                  Biological Hazards                                                             ...
Laboratory for Food Safety    • Specialised in biological (raw or process) and      chemical (environment or process) haza...
EURL CPS, Lm, MMP                                                                                                         ...
Maisons-Alfort Food Safety lab in brief   150 staff   Between 55000 and 85000 analytical controls each year   Annual bu...
Biological and chemical hazards Origin             o in food             o generated by processing or preparation Missions...
European Union Reference Laboratory•        Mandate for Listeria monocytogenes          • Nomination in 2006 (Regulation E...
EURL requirements • suitably qualified staff with adequate training; • equipment and products needed; • appropriate admini...
Role of EURL (1)     • deal with analytical aspects related to the       corresponding legislation              –         ...
Food safety criteria from Annex I                                      (Reg EC 2073/2005)     EURL CPS, Lm, MMP     Europe...
Role of EURL (2) To manage a network of National Reference Laboratories (NRLs)   • 35 NRLs: 1/member state   • Annual work...
Role of EURL (3)     To conduct analytical development       • Internal lab work       • To develop/to improve reference m...
Role of EURL (4)To provide scientific & technical support to EC/DG SANCO          • Upon the needs          • Confirmatory...
EURL CPS, Lm, MMPEuropean Union Reference Laboratory forCoagulase Positive Staphylococci, Listeriamonocytogenes and Milk a...
Focus on activities • Guidance documents (Lm) to provide detailed   for the Reg EC 2073/2005 • Methods:             – Enha...
Article 3                                                  General requirements • 2. As necessary, the food business opera...
Guideline document on shelf-life studies     for Listeria monocytogenes in RTE foods • On demand of DG SANCO • Written in ...
2010-11 EU baseline study in RTE:                              opportunity for Typing database •      Taken part to EFSA p...
Article 5            Specific rules for testing and sampling • Food business operators manufacturing   ready-to-eat foods,...
Guidelines on sampling the food        processing area and equipment for the         detection of Listeria monocytogenes •...
Role of NRL • Very similar to EURL, but at National level 1. To manage the network of laboratories    approved by the Comp...
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"Overview of the European Union reference laboratory (eu rl) for Listeria monocytogenes (eurl) activities" - Adrien Assere (ANSES, Frankreich)

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"Listerien-Referenzlabor: Akademischer Luxus oder Public Health Erfordernis?", 19. Oktober 2012 (AGES, Graz)

Das Nationale Listerien-Referenzlabor ist innerhalb der AGES an das "Zentrum für lebensmittelbedingte Infektionskrankheiten" / Institut für medizinische Mikrobiologie und Hygiene in Graz übersiedelt. Zum Auftakt am neuen Standort veranstaltete die AGES dieses Fachgespräch, bei dem verschiedene Aspekte in Zusammenhang mit dem potentiellen Auftreten von Listerien aktuell beleuchtet wurden. Erstmals wurden zudem die neuen Richtlinien des Europäischen Referenzlabors (EU-RL) für Listeria monocytogenes zur Harmonisierung der Probennahme vorgestellt.

Dokumentation der Fachtagung mit Präsentationen:
http://www.ages.at/ages/ages-akademie/stakeholderveranstaltungen/graz-listerien-referenzlabor/

Published in: Health & Medicine
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"Overview of the European Union reference laboratory (eu rl) for Listeria monocytogenes (eurl) activities" - Adrien Assere (ANSES, Frankreich)

  1. 1. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci Listeria monocytogenes Milk and Milk ProductsMaisons-Alfort Laboratory for Food Safety Overview of the European Union Reference Laboratory (EURL) for Listeria monocytogenes activities in Anses Laboratory Adrien ASSERE Deputy manager of EU reference Laboratories for Listeria monocytogenes, Staphylococci, and Milk & Milk Products Tel.: +33 149 772 749 Adrien.ASSERE@anses.fr EURL CPS, Lm, MMP French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr
  2. 2. French Agency for Food, Environmental and Occupational Health SafetyEURL CPS, Lm, MMPEuropean Union Reference Laboratory forCoagulase Positive Staphylococci, Listeriamonocytogenes and Milk and Milk Products
  3. 3. Relations with the government and ParliamentMinistry for ecology Ministry for Ministry for Ministry for Ministry for and sustainable labour health food, agriculture consumer development and fisheries affairs 5 supervisory ministries State Goals & Resources Contract Vote of the annual grant, annual report Parliament EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  4. 4. Missions Assess nutritional and health Recommend public health risks and benefits measures Monitor health Provide laboratory safety reference missions Conduct, coordinate and Provide training initiate research and information projects and contribute to public debate Coordinate the R 31 Authorise marketing of network of scientific veterinary medicinal organisations products EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products4
  5. 5. ANSES in brief 1350 staff 800 external experts solicited (20 Expert Committees, working groups) Annual budget: 140 million Euros More than 8000 Opinions issued since 1999 51 National Reference Laboratory mandates, 8 EURL mandates 250 scientific publications per year More than 100 doctorate and post-doctorate students EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  6. 6. Pôle HQSA Biological Hazards Pagot Chemical Hazards Bâtiment C Direction/administration Bâtiment B LCSVEURL CPS, Lm, MMPEuropean Union Reference Laboratory forCoagulase Positive Staphylococci, Listeriamonocytogenes and Milk and Milk Products
  7. 7. Laboratory for Food Safety • Specialised in biological (raw or process) and chemical (environment or process) hazards which have an effect on food safety for humans; • Activities: • Reference • Research • Surveillance/epidemiosurveillance • Scientific and technical expertise EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products7
  8. 8. EURL CPS, Lm, MMP HAZARDS European Union Reference Laboratory for Coagulase Positive Staphylococci Listeria monocytogenes Milk and Milk ProductsMaisons-Alfort Laboratory for Bacterial toxins Food Safety Food allergy Mycotoxins Intolerances Toxins Phycotoxins Food poisoning Animal poisons Foodborne Poisons Vegetal poisons diseases Chemical pollutants Bacteria Invasive Parasites Viruses Infections Bacteria Non invasive Parasites EURL CPS, Lm, MMP French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr
  9. 9. Maisons-Alfort Food Safety lab in brief 150 staff Between 55000 and 85000 analytical controls each year Annual budget: 12,6 million Euros 10 NRL mandates, 3 EURL mandates 50 scientific publications per year 30 technical expertise reports per year Around 10 doctorate and post-doctorate students EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products9
  10. 10. Biological and chemical hazards Origin o in food o generated by processing or preparation Missions • To improve knowledge on the identification of hazards • To develop methods for detecting, characterizing and measuring these hazards • To investigate why these hazards appear and what causes their development • To contribute to the implementation of control tools (FBO, OCB) • To assess the risks associated to these hazards • To perform molecular epidemiosurveillance EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products10
  11. 11. European Union Reference Laboratory• Mandate for Listeria monocytogenes • Nomination in 2006 (Regulation EC 882/2004 ammended) • EC Regulation 2073/2005 on microbiological criteria for foodstuffs EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 11
  12. 12. EURL requirements • suitably qualified staff with adequate training; • equipment and products needed; • appropriate administrative infrastructure; • ensure confidentiality; • sufficient knowledge of international standards and practices; • take account of research activities at national and EU level; • trained personnel available for emergency situations occurring within the Community. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products12
  13. 13. Role of EURL (1) • deal with analytical aspects related to the corresponding legislation – Reg. EC 2073/2005 on Microbiological criteria • Criteria for Lm checked by Official Reference EN ISO methods EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products13
  14. 14. Food safety criteria from Annex I (Reg EC 2073/2005) EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products14
  15. 15. Role of EURL (2) To manage a network of National Reference Laboratories (NRLs) • 35 NRLs: 1/member state • Annual workshops • Training sessions • PT inter-laboratory studies • Queries EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products15
  16. 16. Role of EURL (3) To conduct analytical development • Internal lab work • To develop/to improve reference methods for official control EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products16
  17. 17. Role of EURL (4)To provide scientific & technical support to EC/DG SANCO • Upon the needs • Confirmatory analyses • Development/implementation of European legislation • Ex 1: preparation of Reg. 2073/2005 • Ex 2: Implementation of Annex II of Reg. 2073/2005 • Guidelines on challenge tests, durability studies • Food classification • Participation for DG SANCO to European/international technical meetings/activities • Ex: CEN/ISO standardization EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products17
  18. 18. EURL CPS, Lm, MMPEuropean Union Reference Laboratory forCoagulase Positive Staphylococci, Listeriamonocytogenes and Milk and Milk Products
  19. 19. Focus on activities • Guidance documents (Lm) to provide detailed for the Reg EC 2073/2005 • Methods: – Enhancement of Lm enumeration method • filtration • reduction of the enrichment step – PFGE protocole for Lm – EURL Lm DB – PFGE profile database EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products19
  20. 20. Article 3 General requirements • 2. As necessary, the food business operators responsible for the manufacture of the product shall conduct studies in accordance with Annex II in order to investigate compliance with the criteria throughout the shelf-life. In particular, this applies to ready-to-eat foods that are able to support the growth of Listeria monocytogenes and that may pose a Listeria monocytogenes risk for public health. • Food businesses may collaborate in conducting those studies. • Guidelines for conducting those studies may be included in the guides to good practice referred to in Article 7 of Regulation (EC) No 852/2004. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products20
  21. 21. Guideline document on shelf-life studies for Listeria monocytogenes in RTE foods • On demand of DG SANCO • Written in collaboration with representatives of 7 National Reference Laboratories for Listeria monocytogenes (BE, NL, IE, NO, RO, SE, UK) – version 2 (Nov 2008) • Revising after 4 years of practices – new members (SK, PT) EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products21
  22. 22. 2010-11 EU baseline study in RTE: opportunity for Typing database • Taken part to EFSA plans • Opportunity for subtyping the strains • NRLs in charge of collecting the strains • Collaboration of the NRLs to type the strains • 1st use of the EURL database • Work to be transfered to EFSA EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products22
  23. 23. Article 5 Specific rules for testing and sampling • Food business operators manufacturing ready-to-eat foods, which may pose a Listeria monocytogenes risk for public health, shall sample the processing areas and equipment for Listeria monocytogenes as part of their sampling scheme. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products23
  24. 24. Guidelines on sampling the food processing area and equipment for the detection of Listeria monocytogenes • Enquiry • Document drafted by a WG • 137 respondents from 15 EU MSs • Presented after by my colleague Léna BARRE EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products24
  25. 25. Role of NRL • Very similar to EURL, but at National level 1. To manage the network of laboratories approved by the Compentent Authority in to perform official control analyses 2. To perform analytical development 3. Ensure dissemination of information 4. Provide scientific and technical assistance to the competent authority EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products25 B. Lombard 07-03-2012

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