9Th Triennial Conference of APA, Kenya, June30 - July 4, 2013
Can mineral oil protect seeds against
aphid transmission of ...
Potato Seed Problem
• PVY is the most important virus on potato
• 12% of refused production
• Decrease of the yield (40%)
...
POTATO VIRUS Y (PVY)
• Famille : Potyviridae
• Genre : Potyvirus
• More than 30 plant hosts
• Variability of PVY
Potato is...
PVYO
PVYNTNrec +
PVYO
PVYNTNrec
PVYNTN
PVY diversity in seed potato production
in Tunisia
95% PVYNTN
in the laboratory
Efficiency of transmission of 14 Vectors of PVY in Tunisia 
To evaluate the efficiency of the mineral oil
with insecticides as preventive treatment to
control Aphids and PVY infectio...
STEPS OF STUDY
1.Trapping of aphids
2.Treatments of potato (field)
3. Aphid acquisition and transmission of PVY with
oil c...
1. Aphid sampling
• Aerial sampling:
– Suction trap
• Foliage sampling :
– 30 leaves every
week during the
growing season
...
Cap Bon
KorbaINRAT
Soliman
Aerial sampling by suction
trap, daily catches
- Population dynamic
- Frequency
- Risk of conta...
Aphid flight data
0
100
200
300
400
500
600
1/3
8/3
15/3
22/3
29/3
5/4
12/4
19/4
26/4
3/5
10/5
17/5
24/5
31/5
Dates
Aphids...
2. EXPERIMENT1
treatment 1:
insecticides
only
treatment 2:
insecticides +
oil
• Experimental design: 4 repetitions, 3 trea...
Experimental station (Korba)
Detection of PVY on
200 tubers (may)
CT
TC
Evaluation of the
weight of 100 tubers
Control of
...
Treatment results on aphid
populations and PVY infection
Mean numbers of aphid colonies
PVY infections/
200tubers
Insectic...
Treatment reduction of aphids
0%
20%
40%
60%
80%
100%
Treatment 1 Treatment 2
Means followed by common letters are not sig...
Comparison of potato yield in the two
treatments 1,2
33,25
29,75 30,13
26
0
5
10
15
20
25
30
35
Control Treatment1 Control...
to explore the possibility of acquisition and
transmission of PVY by the most efficient
vector Myzus persicae after the oi...
1- Aphids :
• Myzus persicae populations
2 - PVY isolates : PVYNTN on tobacco
3 – Potatoes :
• Transmission
N. tabacum Xan...
Method of transmission
2 hours
to starve 3 mn acquisition of PVYNTN
Inoculation on potato
Plants tests 3 hours
TreatmentDA...
Aphid feeding on infected potato and then moved
on healthy plants
coated with oil
No acquisition,
no transmission
Acquisit...
PVY infection
ELISA results after 15 days inoculation of healthy potato by M. persicae
Aphids that feed
on infected
potato...
Conclusion
• Mineral oil is efficient to reduce PVY transmission
when well coated
• No phytotoxicity observed on plqnt spr...
Future Investigations
• use of crop border
• further evaluation of the use of
biopesticides in combination with oils
• sel...
Thank you
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Sess08 5 boukhris bouhachem s., sellami h.,souissi r. & el fahem m. – can mineral oil protect seeds against aphid transmission of potato virus y

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Sess08 5 boukhris bouhachem s., sellami h.,souissi r. & el fahem m. – can mineral oil protect seeds against aphid transmission of potato virus y

  1. 1. 9Th Triennial Conference of APA, Kenya, June30 - July 4, 2013 Can mineral oil protect seeds against aphid transmission of Potato virus Y? Boukhris-Bouhachem S., Sellami H., Souissi R. & El Fahem M. GIL
  2. 2. Potato Seed Problem • PVY is the most important virus on potato • 12% of refused production • Decrease of the yield (40%) • PVY is transmitted by more than 70 aphid species with non-persistent manner • Emergence of virus strain decreasing the quality of potato seeds PVYNTN , PVYNW
  3. 3. POTATO VIRUS Y (PVY) • Famille : Potyviridae • Genre : Potyvirus • More than 30 plant hosts • Variability of PVY Potato isolates PVYO PVYC PVYN PVYNTN PVYNW 95% PVYNTN
  4. 4. PVYO PVYNTNrec + PVYO PVYNTNrec PVYNTN PVY diversity in seed potato production in Tunisia 95% PVYNTN
  5. 5. in the laboratory Efficiency of transmission of 14 Vectors of PVY in Tunisia 
  6. 6. To evaluate the efficiency of the mineral oil with insecticides as preventive treatment to control Aphids and PVY infection of potato OBJECTIVES High quality of seeds
  7. 7. STEPS OF STUDY 1.Trapping of aphids 2.Treatments of potato (field) 3. Aphid acquisition and transmission of PVY with oil coat plants (in laboratory)
  8. 8. 1. Aphid sampling • Aerial sampling: – Suction trap • Foliage sampling : – 30 leaves every week during the growing season Suction trap
  9. 9. Cap Bon KorbaINRAT Soliman Aerial sampling by suction trap, daily catches - Population dynamic - Frequency - Risk of contamination - Treatment decision FOLLOWED APPROACHES
  10. 10. Aphid flight data 0 100 200 300 400 500 600 1/3 8/3 15/3 22/3 29/3 5/4 12/4 19/4 26/4 3/5 10/5 17/5 24/5 31/5 Dates Aphids/day Thiamethoxam Imidacloprid Pymethrozine Haulm destruction
  11. 11. 2. EXPERIMENT1 treatment 1: insecticides only treatment 2: insecticides + oil • Experimental design: 4 repetitions, 3 treatments (T, I, P) • Elementary plots: 625 m2 (5mx1000m) separated by 1m to avoid interactions
  12. 12. Experimental station (Korba) Detection of PVY on 200 tubers (may) CT TC Evaluation of the weight of 100 tubers Control of phytotoxicity
  13. 13. Treatment results on aphid populations and PVY infection Mean numbers of aphid colonies PVY infections/ 200tubers Insecticides Thiamethoxam Imidacloprid Pymetrozine Dates 7/4 14/4 21/4 12/5 28/4 5/5 12/10 Treatment 1a 9 6 25 27 13 31 9,5% Control 1 b 35 43 215 50 102 103 12% Treatment 2 a 21 9 12 26 15 34 2.5% Control 2 b 66 52 193 79 89 158 4% Treatments with different letter are significantly different (p=0.05, ANOVA) (+): positive samples by DAS-ELISA
  14. 14. Treatment reduction of aphids 0% 20% 40% 60% 80% 100% Treatment 1 Treatment 2 Means followed by common letters are not significantly different at 5% level Aphids aa
  15. 15. Comparison of potato yield in the two treatments 1,2 33,25 29,75 30,13 26 0 5 10 15 20 25 30 35 Control Treatment1 Control Treatment 2 Potato yield (kg) a a a a
  16. 16. to explore the possibility of acquisition and transmission of PVY by the most efficient vector Myzus persicae after the oil application on potato plants. EXPERIMENT 2
  17. 17. 1- Aphids : • Myzus persicae populations 2 - PVY isolates : PVYNTN on tobacco 3 – Potatoes : • Transmission N. tabacum Xanthi -> Potatoes Aphid transmission in the laboratory
  18. 18. Method of transmission 2 hours to starve 3 mn acquisition of PVYNTN Inoculation on potato Plants tests 3 hours TreatmentDAS-ELISA après 15 jours 15 days ELISA
  19. 19. Aphid feeding on infected potato and then moved on healthy plants coated with oil No acquisition, no transmission Acquisition, transmission Acquisition, no transmission coated with oil
  20. 20. PVY infection ELISA results after 15 days inoculation of healthy potato by M. persicae Aphids that feed on infected potato treated with oil then sucking on healthy plants Infected aphids feeding on healthy plants treated with mineral oil infected aphids feeding on potato healthy plants without oil treatment Infected potato plants 0 (+)/40 0(+)/40 38 (+)/40
  21. 21. Conclusion • Mineral oil is efficient to reduce PVY transmission when well coated • No phytotoxicity observed on plqnt sprqyed with oil • Application of oil have no negative effect on the yield
  22. 22. Future Investigations • use of crop border • further evaluation of the use of biopesticides in combination with oils • selection of resistant plant to aphids and PVY
  23. 23. Thank you

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