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Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
Micropropagation. adil.
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Micropropagation. adil.

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micropropagation the process of generating plant from a small part of the plant

micropropagation the process of generating plant from a small part of the plant

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  • 1. MICROPROPAGATION PRESENTED BY ADIL MAZEED B.SC BIOTECHNOLOGY 3 RD YEAR
  • 2. Definition of micropropagation Clonal propagation ‘IN VITRO’  Micro-propagation is the production of whole plant from small section of plant such as a stem tip, node, meristem, embryo, or even a seed.
  • 3. STEPS OF MICRO-PROPAGATION  Establishment  Proliferation  Rooting and hardening  Sometimes one step is also included in cases where establishment of plantlets in soil is particularly elaborate
  • 4. Selection of an elite mother plant STEP 1- ESTABLISHMENT- In this step, selection of suitable plants, their sterilization and transfer to nutrient media for establishment, i.e. initiation of a sterile culture of the explant. STEP 2 – PROLIFERATION- Proliferation or multiplication of shoot from the explant on medium. STEP 3 – ROOTING & HARDNING- Transfer of shoots to a rooting medium followed later by planting into soil Explant Surface sterilization and washing Establishment on growth medium Transfer to proliferation medium Shoot formation Transfer of shoot or plantlets to sterilized soil or artificial medium by various gradual weaning processes
  • 5.  METHODS OF MICRO-PROPAGATION  AXILLARY BUD PROLIFERATION APPROACH • Meristem and shoot tip culture • Bud culture  ORGANOGENESIS • INDIRECT • DIRECT  EMBRYOGENESIS
  • 6.  AXILLARY BUD PROLIFERATION APPROACH Meristem and shoot tip culture Morel and Martin (1952) develop the technique of meristem culture for in vivo virus of Dahlia.  G. Morel (1965) was developed the technique shoot tip culture for micro propagation of orchid Cymbidium.  This method is more successful in herbaceous plant.  Bud culture Buds contain active meristem depending upon the physiological state of the plant. The various types used in bud culture, a). Single node culture b). Axillary bud method
  • 7.  ORGANOGENESIS  Indirect This pathway includes a callus stage.  Callus is undifferentiated tissue that develops on or around an injured or cut plant surface.  Direct This pathway is bypasses a callus stage.  This method is particularly suitable to herbaceous species .
  • 8.  EMBRYOGENESIS • The process of initiation and development of embryos and embryo like structure from somatic cells. • It usually involves a callus intermediate stage which can result in variation among seedlings. • Its not a common micro-propagation technique but currently being used to produce superior pine seedlings. a). Direct embryogenesis b). Indirect embryogenesis
  • 9. HOW does micro-propagation work?  Plant cell have the ability to reproduce the whole plant from a single cell. This is called totipotency.  Totipotency is the ability of single cell to express the full genome in the cells to which it gives rise by cell division. Why do we do it?  To regenerate plant from single cell or plant tissues.  To produce large quantities of identical plant.  To create new plant varieties.
  • 10. thank you

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