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Effect of physical properties of cotton on enzyme concentration
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Effect of physical properties of cotton on enzyme concentration

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Effect of physical properties of cotton on enzyme concentration Effect of physical properties of cotton on enzyme concentration Presentation Transcript

  • INFLUENCE OF ENZYMES ON TEXTILE WET PROCESSING 1 Aravin Prince Periyasamy aravinprince@gmail.com
  • ENZYMES Enzymes - Greek word “Enzymos” Meaning - in the cell / from the cell’. Enzymes – Proteins composed of amino acids, produced by all living organisms Responsible for number of reactions & biological activities. Enzymes - Work efficiently and rapidly & biodegradable. Enzymes types - Malt, Bacterial, Pancreatic etc, Major functions - Hydrolysis, Oxidation, Reduction Coagulation & Decomposition. 2
  • HYDROLASES ENZYME Hydrolases - Mostly used enzyme in textiles. These enzymes are mostly preferred due to following reasons 1) Accelerate the rate of the reaction 2) Specific in action 3) Low temperature operation 4) Safe and easy to control. 5) Replace harsh chemicals 6) No pollution 7) Biologically degradable 3
  • CHARACTERISTICS OF ENZYMESUsed during the process & can berecovered at the endRequires low activation energy - reactionconditions are close to the ambient.Sharp control over process conditions - verysensitive to pH & temp variations.Eco-friendly & accomplish their work quicklyand efficiently without leaving any pollutantsbehind. 4
  • ENZYMATIC MECHANISMFirst suggested by Emil Fischer in 1890s.Enzymes - particular shape - substrate fitexactly like “Lock and Key” model.The active centers in the enzymes (Fissures,Holes, Pockets, Cavities or Hollows) formcomplex with the substrate.The bio-reaction in this complex & itsdestruction - release of original enzyme and 5the product.
  • Enzymatic mechanism 6
  • MATERIAL SPECIFICATIONS 2 types of fabrics Plain cotton woven fabric - desizing, Scouring, Bio-polishing 3/1 twill weave Denim fabric - Denim washing Plain cotton woven Denim fabric fabricWeave : Plain Woven Weave : 3/1 Twill Fabric Woven FabricEPI : 80 EPI : 26PPI : 60 PPI : 20GSM : 130 GSM : 180Warp Count : 60s Ne Warp Count : 18sNeWeft Count : 60s Ne Weft Count : 26s Ne
  • ENZYMATIC DESIZINGMost effective & widely used method for theremoval of Starch.Use Starch liquefying enzymes in requiredquantity under controlled condition of pH andtemperature.Amylase enzyme from Malt extract were firstused to degrade starch-based sizes for cheapand effective desizing.Two categories of amylase α-amylase β-amylase 8
  • ENZYMATIC DESIZING (Contd…) α-amylase attacks the starch randomly &form Dextrins. Very rapid in action & producesimple water soluble sugars. The β-amylase converts starch into maltose& it is slower in the rate of action. The rate of desizing depends on the ratio ofthese two amylases. Higher α-amylase content - faster reaction & vice versa. 9
  • MECHANISM OF DESIZING BY α- AMYLASE ENZYME
  • PROCESS SEQUENCESRecipe α Amylase enzyme – 3%, 6% & 9% o.w.f wetting agent – 0.5% o.w.f pH – 8-10 M:L- 1:40 Temp - 55°c Time – 1 hrSequence Treat the fabric with the given recipe Raise the temp of the bath to 55°c for 1 hrTaken out the material and rinse with hot & cold water Squeeze 11 Dry
  • Enzymatic desizing EFFECT OF AMYLASE ON DESIZING EFFICIENCY Enzyme used: Amylase EnzymeS. Enzyme Conc. Untre 3% 6% 9% Acidno & Parameters ated Desizi ng1 Desizing 0 90 94 98 85 efficiency (%)2 Strength Loss 48 47.3 46.5 46 44 (Kg)
  • EFFECT OF AMYLASE ON DESIZING EFFICIENCY120100 Untreated80 3%60 6%40 9%20 Acid Desizing 0 Desizing Efficiency Strength Losss
  • BIO-SCOURING Alkaline scouring – require harsh chemicals, high temp & high effluent load Bio-scouring - effective alternative to chemical scouring. Cottonase T & Pectinease enzyme is widely used Versatile, economically viable & eco- friendly 14
  • PROCESS SEQUENCES Enzyme used Conc % (owf) pH Temp (°c) Recipe: Pectinease 1.5, 3 & 4.5% 6.0 60 Cottanase T 1.5, 3 & 4.5% 6.5 65 Sequence Treat the fabric with the given recipe Raise the temp of the bath to 55°c for 1 hr Taken out the material and rinse with hot & cold water Squeeze dry 15
  • BIO SCOURING EFFECT OF PECTINASE ON SCOURING EFFICIENCYSl.No Desizing 3 6 9 conc (%) Pectinase 1.5 3 4.5 1.5 3.0 4.5 1.5 3.0 4.51 Conc (%) Absorbency 3.3 3 2.9 2.8 2.6 2.5 2 1.8 1.62 (Sec)
  • EFFECT OF PECTINASE ON SCOURING EFFICIENCY 3.5 3 Absorbency (Sec) 2.5 2 1.5 1 0.5 0 1.5 3 4.5 Pectinase Conc.(%) Amylase 3 % Amylase 6 % Amylase 9 %
  • BIO SCOURING EFFECT OF COTTANASE T ON SCOURING EFFICIENCYSl.No Desizing 3 6 9 conc (%) Cottonase 1.5 3 4.5 1.5 3.0 4.5 1.5 3.0 4.51 Conc (%) Absorbency 4.4 4.2 4.1 3.7 3.5 3.5 3.0 2.7 2.62 (Sec)
  • EFFECT OF COTTANASE T ON SCOURING EFFICIENCY 5 4.5 4 Absorbency (Sec) 3.5 3 2.5 2 1.5 1 0.5 0 Cottanase T 20 Conc.(%) 1.5 3 4.5 Amylase 3 % Amylase 6 % Amylase 9 %
  • ADVANTAGES OF BIO-SCOURING Milder conditions of processing and excellent absorbency in goods. No Oxy-cellulose formation and less strength loss Uniform removal of waxes. Highly suitable for scouring of blends. Low TDS in discharge. Compared to conventional fabric is softer 20
  • APPLICATION OF ENZYME ON FINISHING Finishing - value addition process - hidesfabric defects & improves aesthetic value.Enzymes application area in finishing process Bio-washing Bio-polishingThe fashionable stone wash look of denimgarment and other processes – applications ofcellulose finishing. 21
  • DENIM WASHINGDenim fabric - woven fabric of 3/1 twillweave coarse counts of yarn Warp - Indigo dyed yarn Weft- Grey yarn.Conventional method - faded effectachieved by acid / stone wash.Stone wash – garment/fabric rubbedagainst pumice stonesDisadv: loss of strength & thickness of thefabric.
  • MECHANISM OF DENIM WASHING 23
  • PROCESS SEQUENCESEnzyme used Conc % (owf) pH Temp (°c) Recipe: Laccase 1, 1.5 & 2% 6.0 40 Sequence Load the fabric in drum dyeing machine Add the water & wetting agent Raise the temperature 60 0 C Run the fabric for 20 min Drain Add the water and Laccase enzyme Run the fabric for 60 min Drain Cold wash 24 Dry
  • DENIM WASHING (Contd…) Enzyme used - Cellulase enzymes Advantages Completely eliminate stone as the abrasive agent in denim wash Prevent damage to washing machines & garments Eliminates disposal of the used stones Improves the quality of the waste water No environmental pollution Reduces processing time Saves chemical & energy costs
  • BIO WASHING EFFECT OF LACCASE ON BIO WASHING EFFICIENCYEnzyme used: Laccase Enzyme Enzyme Conc Used %(o.w.f) STONEParameters STONE + 1% 1.5% 2% WASH ENZYME WASHWeight loss % 2% 2.5% 3% 10% 12%
  • % of Weight loss en zy m e 10 12 14 0 2 4 6 8 c o n c 1 % en zy m e c o n c 1 .5 % en zy m e c o n c 2 % st o EFFICIENCY ne w a sh st o ne +e nz ym eweight loss% EFFECT OF LACCASE ON BIO WASHING
  • BIO POLISHINGTo remove every kind of impurity + endsprotruding from the fabric surface.Enzymatic hydrolysis weakens the fiber ends.To achieve a good result, mechanicaltreatment is necessary.Cellulase enzyme – widely usedCause controlled super ficial attack on thefiber leading to decolourisation without bringingabout excessive loss in fabric strength.
  • PROCESS SEQUENCESEnzyme used Conc % (owf) pH Temp (°c) Recipe: Cellulase 1, 1.5 & 2% 6.5 45 Sequence Load the fabric in drum dyeing machine Add the water & wetting agent Raise the temperature 60 0 C Run the fabric for 20 min Drain Add the water and Laccase enzyme Run the fabric for 60 min Drain Cold wash 29 Dry
  • EFFECT OF BIO POLISHINGBefore enzyme treatment After enzyme treatment
  • BIO POLISHING EFFECT OF CELLULASE ON BIO POLISHING EFFICIENCYEnzyme used: Cellulase Enzyme Enzyme Conc Used %(o.w.f) Parameters 1% 1.5% 2% Weight loss % 1.5% 2% 3%
  • EFFECT OF CELLULASE ON BIO POLISHING EFFICIENCY 3.5 3 2.5 Enzyme Conc % 2 1.5 1 0.5 0 1 1.5 2 Weight loss % weight loss %
  • BIO POLISHING (Contd…) ADVANTAGES Reduces the pilling tendency Cleans the fabric surface & reduces fluff Improves handle properties like softness & flexibility Improves fabric drape Improves the hydrophilic properties Improves dyestuff affinity, yield, levelness & brilliance
  • CONCLUSIONThus the “ENZYMES” replaces traditionalmethod with more advantages such as Replacing harmful chemicals Require less effluent treatments Low cost and less energy consumption No damages to materials 34
  • REFERENCES Application of Bio-Technology in Textiles/ Asian Dyer Oct 06 Application of enzyme in textile processing – by Dr Bhaarathi dhurai www.fiber2fashion.com/articles/biotech.453h tml www.biocon.com/products/textilefinishing www.resil.com/products www.mapsenzyme.com/developments www.bharattextile.com/newsletter www.textileinfo.com/enzymes
  • REFERENCE Application of Bio-Technology in Textiles/ Asian Dyer Oct 06 Application of enzyme in textile processing – by Dr Bhaarathi dhurai www.fiber2fashion.com/articles/biotech.453html www.biocon.com/products/textilefinishing www.resil.com/products www.mapsenzyme.com/developments www.bharattextile.com/newsletter www.textileinfo.com/enzymes 36