Antibody engineering-Abbas Morovvati

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Antibody engineering-Abbas Morovvati

  1. 1.  Introduction Structure of antibody Engineering Anti body monocolonal and poly colonal Humanizing antibody Refrence
  2. 2.  Your immune system makes proteins called ANTIBODIES Antibodies are produced by cells called B- lymphocytes B lymphocytes are produced when your immune system detects a foreign substance that has invaded your body
  3. 3.  Polyclonal antibody  Monoclonal antibody › Antigens possess multiple › Derived from a single epitopes clone, specific for a single › Serum antibodies are epitope heterogeneous, › For most research,  To increase immune diagnostic, and protection in vivo therapeutic purposes  To reduces the efficacy of antiserum for various in vitro uses › To response facilitates the localization, phagocytosis, and complement- mediated lysis of antigen › To have clear advantages for the organism in vivo
  4. 4. CLASSES  IgM, IgG, IgD, IgA, IgE  SUBCLASSES IgG1, IgG2, IgG3, IgG4
  5. 5. MONOMER PENTAMER MONOMER DIMER MONOMER MONOMER WHEN ACTING AS B-CELL RECEPTOR FOR ANTIGEN
  6. 6. Light Chain Heavy Chain Fab Fc
  7. 7. Mu Mu Human Chimeric Antibody
  8. 8. MouseVL VH Human VL VHHumanized Antibody with Mouse Loops VL VH
  9. 9.  • First step - development of  hybridomas monoclonal antibodies  – Parallel work with isolated – Fusion of antibody- Fab fragments in bacteria producing B cell with  • Third step - re-engineering myeloma for desired properties – Results in immortalized  – Reducing immunogenicity monospecific Ab-producing of mouse antibodies cell  – Tailoring size and half-life line for specific need • Second step - ability to  – Adding or removing clone and re-express Abs functions – Initially done with cloned, rearranged genes from
  10. 10.  1-Berezov A, Zhang HT, Greene MI, Murali R. 2001. Disabling erbB receptors with rationally designed exocyclic mimetics of antibodies: Structure- function analysis. J Med Chem 44:2565-2574. 2-Drebin JA, Link VC, Greene MI. 1988. Monoclonal antibodies specific for the neu oncogene product directly mediate anti-tumor effects in vivo. Oncogene 2:387-394. 3-Norman C. Peterson Advances in Monoclonal Antibody Technology: Genetic Engineering of Mice, Cells, and Immunoglobulins 4-Sang Jick Kim, Youngwoo Park, and Hyo Jeong Hong-Antibody Engineering for the Development of Therapeutic Antibodies(Received August 16, 2005; Accepted August 18, 2005) 5-Roland E KONTERMANN Recombinant bispecific antibodies for cancer therapy (2005 Jan) 6-Harris JM, Chess RB. Effect of pegylation on pharmaceuticals. Nat Rev Drug Discov 2003;
  11. 11.  7-M. FRANEK, K. HRUSKA Antibody based methods for environmental and food analysis: a review (Czech, 50, 2005) 8-Laschi S., Franek M., Mascini M. (2000): Screen-printed electrochemical immunosensors for PCB detection. Electroanalysis, 9-Zeravik J., Skryjova K., Nevorankova Z., Franek M. (2004): Development of direct ELISA for the determination of 4-nonylphenol and octylphenol. Analytical Chemistry,

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