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Antibody engineering-Abbas Morovvati
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Antibody engineering-Abbas Morovvati

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Antibody engineering-Abbas Morovvati Antibody engineering-Abbas Morovvati Presentation Transcript

  •  Introduction Structure of antibody Engineering Anti body monocolonal and poly colonal Humanizing antibody Refrence
  •  Your immune system makes proteins called ANTIBODIES Antibodies are produced by cells called B- lymphocytes B lymphocytes are produced when your immune system detects a foreign substance that has invaded your body
  •  Polyclonal antibody  Monoclonal antibody › Antigens possess multiple › Derived from a single epitopes clone, specific for a single › Serum antibodies are epitope heterogeneous, › For most research,  To increase immune diagnostic, and protection in vivo therapeutic purposes  To reduces the efficacy of antiserum for various in vitro uses › To response facilitates the localization, phagocytosis, and complement- mediated lysis of antigen › To have clear advantages for the organism in vivo
  • CLASSES  IgM, IgG, IgD, IgA, IgE  SUBCLASSES IgG1, IgG2, IgG3, IgG4
  • MONOMER PENTAMER MONOMER DIMER MONOMER MONOMER WHEN ACTING AS B-CELL RECEPTOR FOR ANTIGEN
  • Light Chain Heavy Chain Fab Fc
  • Mu Mu Human Chimeric Antibody
  • MouseVL VH Human VL VHHumanized Antibody with Mouse Loops VL VH
  •  • First step - development of  hybridomas monoclonal antibodies  – Parallel work with isolated – Fusion of antibody- Fab fragments in bacteria producing B cell with  • Third step - re-engineering myeloma for desired properties – Results in immortalized  – Reducing immunogenicity monospecific Ab-producing of mouse antibodies cell  – Tailoring size and half-life line for specific need • Second step - ability to  – Adding or removing clone and re-express Abs functions – Initially done with cloned, rearranged genes from
  •  1-Berezov A, Zhang HT, Greene MI, Murali R. 2001. Disabling erbB receptors with rationally designed exocyclic mimetics of antibodies: Structure- function analysis. J Med Chem 44:2565-2574. 2-Drebin JA, Link VC, Greene MI. 1988. Monoclonal antibodies specific for the neu oncogene product directly mediate anti-tumor effects in vivo. Oncogene 2:387-394. 3-Norman C. Peterson Advances in Monoclonal Antibody Technology: Genetic Engineering of Mice, Cells, and Immunoglobulins 4-Sang Jick Kim, Youngwoo Park, and Hyo Jeong Hong-Antibody Engineering for the Development of Therapeutic Antibodies(Received August 16, 2005; Accepted August 18, 2005) 5-Roland E KONTERMANN Recombinant bispecific antibodies for cancer therapy (2005 Jan) 6-Harris JM, Chess RB. Effect of pegylation on pharmaceuticals. Nat Rev Drug Discov 2003;
  •  7-M. FRANEK, K. HRUSKA Antibody based methods for environmental and food analysis: a review (Czech, 50, 2005) 8-Laschi S., Franek M., Mascini M. (2000): Screen-printed electrochemical immunosensors for PCB detection. Electroanalysis, 9-Zeravik J., Skryjova K., Nevorankova Z., Franek M. (2004): Development of direct ELISA for the determination of 4-nonylphenol and octylphenol. Analytical Chemistry,