(How Much)

Do We Know

?
Q-1
• Who had a flair for working with
lenses and discovered the basic
instrument of microbiology
• A. Robert Koch
• B. Jo...
Microscope
• Antony van leeuwenhoek –
father of microbiology
• Draper
• Developed interest in lens
making from glass and
o...
Q-2
• Identify the gentleman
Vaccination
• Milkmaids who had
occupational exposure to cow
pox did not get small pox
• Edward Jenner
• observed that whe...
Q-3
• Which branch of microbiology
deals with blood grouping?
• A. immunology
• B. serology
• C. Virulogy
• D. Antigen-ant...
Serology
• Study of these antibodies and
antigens in body fluids
• Blood grouping
Q-4
• Which among the following
statements is true?
• A. Fungi are eukaryotes
• B. viruses have cell wall
• C. Bacteria ha...
Classification of
Microorganisms
• Classification by structure
• Subcellular – DNA or RNA surrounded by
a protein coat – v...
• Protozoans – single celled
eukaryotes
• Fungi – multicelled eukaryotes
with cell wall
• Parasites – multicelled
eukaryot...
Classification of
Microorganisms
• Structure
Q-5
• Which class of organism causes
the following illness
•
•
•
•

A.
B.
C.
D.

Virus
Bacteria
fungi
Protozoa
Protozoans
• Single-celled eukaryotic organisms,
larger than bacteria
• Found in soil and water
• Illnesses

• Malaria
• A...
Q-6
• What is the type of culture seen
in next picture used for?
•
•
•
•

A.
B.
C.
D.

Antibiotic Specificity Testing
Antibody Sensitivity testing
Growing in pure culture
Antibiotic sensi...
Determining
Antimicrobial Sensitivity
Q-7
• What is the unit of
measurement of a bacterium
cell wall?
• A. meter
• B. Nanometer
• C. Micrometer
• D. centimeter
Size of Bacteria
• Unit of measurement in bacteriology
is the micron (micrometre, µm)
• 1 micrometre (10-6)= 1/1000 mm =
1...
Anatomy of a
Bacterial Cell
CELL WALL
•

Outermost layer, encloses cytoplasm
1.Confers shape and rigidity
2.10 - 25 nm thick

3.Composed of peptidogly...
Q-8
• What is the process of
reproduction of bacteria known
as?
• A. replication
• B. regeneration
• C. duplication
• D. B...
Binary Fission
• DNA replication
• Plasma membrane invaginate
• Cell wall deposited in
invaginated space
• Cross wall comp...
Binary Fission
• Light micrograph
Binary Fission
Consequences of Binary
Fission
• Very large number of cells very
fast
• Mathematical progressions
• arithmetic
(1>2>4>6>8>...
Q-9
• During which phase of bacterial
growth curve, is the growth
fastest?
• A. Lag phase
• B. Log phase
• C. Death phase
...
Bacterial Growth Curve
Stationary phase
Death
phase

Log phase

Lag phase
1

5
Time (hours)

10
Final question…Thank
God!
• Which type of liquid culture
medium contains an inhibitory
substance added to it?
• A. Enriche...
Enriched media
• Substances like blood, serum, egg
are added to the basal medium.
• Used to grow bacteria that are
exactin...
Blood agar

Chocolate agar
Enrichment media
• Liquid media used to
isolate pathogens from a
mixed culture.
• Media is incorporated
with inhibitory
su...
Selective media
• The inhibitory substance is added
to a solid media.
Eg:
• Mac Conkey’s medium for gram
negative bacteria...
Mac Conkey’s medium

TCBS
Potassium Tellurite media

LJ media
Indicator media
• These media contain an indicator
which changes its colour when a
bacterium grows in them.
• Eg:
• Blood ...
Urease medium
Hope u enjoyed
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This is a series of lectures on microbiology useful for undergraduate medical and paramedical students

Published in: Health & Medicine, Technology
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  • Chocolate agar
  • TCBS
  • C.Diphtheriae on Potassium tellurite media
  • Mac Conkey’s medium
  • Quiz 1

    1. 1. (How Much) Do We Know ?
    2. 2. Q-1 • Who had a flair for working with lenses and discovered the basic instrument of microbiology • A. Robert Koch • B. Joseph Lister • C. Antony Van Leuuewenhoek • D. Christian Gram
    3. 3. Microscope • Antony van leeuwenhoek – father of microbiology • Draper • Developed interest in lens making from glass and observing things through them • Little animalcules
    4. 4. Q-2 • Identify the gentleman
    5. 5. Vaccination • Milkmaids who had occupational exposure to cow pox did not get small pox • Edward Jenner • observed that when extract from cowpox lesion was injected into a person unaffected by small pox, it gave immunity
    6. 6. Q-3 • Which branch of microbiology deals with blood grouping? • A. immunology • B. serology • C. Virulogy • D. Antigen-antibody reactions
    7. 7. Serology • Study of these antibodies and antigens in body fluids • Blood grouping
    8. 8. Q-4 • Which among the following statements is true? • A. Fungi are eukaryotes • B. viruses have cell wall • C. Bacteria have nucleus • D. Parasites are prokaryotes
    9. 9. Classification of Microorganisms • Classification by structure • Subcellular – DNA or RNA surrounded by a protein coat – viruses • Prokaryotic – simple cell structure with no nucleus or organelles – bacteria • Eukaryotic – complex cell structure with nucleus and specialized organelles – protozoans, fungi, parasites
    10. 10. • Protozoans – single celled eukaryotes • Fungi – multicelled eukaryotes with cell wall • Parasites – multicelled eukaryotes without cell wall
    11. 11. Classification of Microorganisms • Structure
    12. 12. Q-5 • Which class of organism causes the following illness
    13. 13. • • • • A. B. C. D. Virus Bacteria fungi Protozoa
    14. 14. Protozoans • Single-celled eukaryotic organisms, larger than bacteria • Found in soil and water • Illnesses • Malaria • Amebic dysentery • Trichomoniasis vaginitis • Leading cause of death in developing countries
    15. 15. Q-6 • What is the type of culture seen in next picture used for?
    16. 16. • • • • A. B. C. D. Antibiotic Specificity Testing Antibody Sensitivity testing Growing in pure culture Antibiotic sensitivity testing
    17. 17. Determining Antimicrobial Sensitivity
    18. 18. Q-7 • What is the unit of measurement of a bacterium cell wall? • A. meter • B. Nanometer • C. Micrometer • D. centimeter
    19. 19. Size of Bacteria • Unit of measurement in bacteriology is the micron (micrometre, µm) • 1 micrometre (10-6)= 1/1000 mm = 1/10000 cm = 1/100000 metre • 1 nanometer (10-9)= 1/1000 micrometer = 1/100000000 meter • Bacteria of medical importance • 0.2 – 1.5 µm in diameter • 3 – 5 µm in length
    20. 20. Anatomy of a Bacterial Cell
    21. 21. CELL WALL • Outermost layer, encloses cytoplasm 1.Confers shape and rigidity 2.10 - 25 nm thick 3.Composed of peptidoglycan
    22. 22. Q-8 • What is the process of reproduction of bacteria known as? • A. replication • B. regeneration • C. duplication • D. Binary fission
    23. 23. Binary Fission • DNA replication • Plasma membrane invaginate • Cell wall deposited in invaginated space • Cross wall completed • Cells separate
    24. 24. Binary Fission • Light micrograph
    25. 25. Binary Fission
    26. 26. Consequences of Binary Fission • Very large number of cells very fast • Mathematical progressions • arithmetic (1>2>4>6>8>10>12>14>16) • geometric(1>2>4>8>16) • exponential expression (20 > 21 > 22 >23>24) • logarithmic expression(0 >log21>log22>log23>log24)
    27. 27. Q-9 • During which phase of bacterial growth curve, is the growth fastest? • A. Lag phase • B. Log phase • C. Death phase • D. Stationary phase
    28. 28. Bacterial Growth Curve Stationary phase Death phase Log phase Lag phase 1 5 Time (hours) 10
    29. 29. Final question…Thank God! • Which type of liquid culture medium contains an inhibitory substance added to it? • A. Enriched Media • B. Enrichment Media • C. Selective Media • D. Indicator Media
    30. 30. Enriched media • Substances like blood, serum, egg are added to the basal medium. • Used to grow bacteria that are exacting in their nutritional needs. • Eg: Blood agar, Chocolate agar
    31. 31. Blood agar Chocolate agar
    32. 32. Enrichment media • Liquid media used to isolate pathogens from a mixed culture. • Media is incorporated with inhibitory substances to suppress the unwanted organism. • Eg: • Selenite F Broth – for the isolation of Salmonella, Shigella • Alkaline Peptone Water –
    33. 33. Selective media • The inhibitory substance is added to a solid media. Eg: • Mac Conkey’s medium for gram negative bacteria • TCBS – for V.cholerae • LJ medium – M.tuberculosis • Wilson and Blair medium – S.typhi • Potassium tellurite medium – Diphtheria bacilli
    34. 34. Mac Conkey’s medium TCBS
    35. 35. Potassium Tellurite media LJ media
    36. 36. Indicator media • These media contain an indicator which changes its colour when a bacterium grows in them. • Eg: • Blood agar • Mac Conkey’s medium • Christensen’s urease medium
    37. 37. Urease medium
    38. 38. Hope u enjoyed
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