Purposes:-Point-of-care Medical Applications-Cell characterization-Disease Detection-Biological SeparationAdvantages:-Cost effective in a large variety of circumstances-Can operate with minimal support (all on-chip)-High surface-area-to-volume ration (catalysis)Limitations:-Field of View problems for detection-Usually require fluorescence-Decision Algorithms could be improved
The proposed detection method was faster and cheaper than flow cytometry for characterizing micro particles.LUCAS = Lensless, Ultra-wide Cell Monitoring Array platform based on Shadow Imaging
Equation was developed for characterizing overlapping cells, which wasn’t a problem below 50,000 cells/second flow. Plane gaps between cells were required to be 100 micrometer or greater.Proven for both Homogeneous mixtures of different sizes and heterogeneous mixtures.Methods developed include: Surface Chemistry, Dielectric Micro Bead, and Fluorescent particle labeling
PEEK = Polyether Ether Ketone. Throughout the paper, they talk about the advantages and disadvantages of using PEEK, including the development of a new bonding process to overcome disadvantages. C4D = Capacitively Coupled Contactless Conductivity Measurements, which uses a signaling and detection electrode.MinCE = Completely Minimized Capillary Electrophoresis Device encompassing entire experimental apparatus in hand-held form.
Food and Beverage – Characterized organic acids in wine and saccharides in fruit juices.Biological Analysis – Detection of 13 amino acids in acidic media.Medical Analysis – Detection of Lithium levels in blood, which Li+ is used as a treatment for manic-depressives.DNA Applications – Two separate DNA fragments were detected in electrophoresis gel medium.
The use of PEEK, higher through-put designs, complex detection algorithms, and electrical detection methods opens the door for expanded applications. Applications include: DNA detection, Biological Analysis, Point-of-care Medical Analysis, and Food/Beverage Analysis to name a few.The literature shows that researchers are overstepping the limiting factors of microdevices. Expanded through-put and more complex detection methods are developing to create the next generation of microdevices.
Minerick Final Presentation April 23rd 2010
Innovations in the Design and Detection of Polymer Lab-on-a-chip Microfluidic Devices<br />Presented By: Aaron Ritthaler<br />
Background<br />Microfluidic Devices serve many purposes<br />Many advantages to this technology<br />Limited by throughput and detection methodology<br />
Literature Reviews<br />High-throughput Lens-Free Imaging and Characterization of a Heterogeneous Cell Solution <br />Polymer Lab-on-a-Chip System with Electrical Detection<br />
High-throughput Lens-Free Imaging and Characterization of a Heterogeneous Cell Solution <br />Goal: Replace Flow Cytometry with microfluidic detection device<br />Built off of previous work (LUCAS)<br />Focus on decision algorithm<br />
Experiment<br />Imaged polystyrene beads and a variety of cells<br />No external fluid flow during imaging<br />Depth of field was 4 mm<br />Area of field was 10 cm2<br />“White” light source used to generate cell shadow images<br />
Results<br />Algorithm is limited by detection of overlapping cells and plane gaps<br />Proven detection and separate characterization of three micro particles<br />Methods developed to characterize undetectable cells<br />
Polymer Lab-on-a-Chip System with Electrical Detection<br />Goal: Present new materials and methods for Lab-on-a-chip devices<br />Supports the use of PEEK for fabrication<br />Demonstrates CCD (C4D) detection method<br />Developed “MinCE”<br />
Results<br />Successful in all experiments as a “proof of concept”<br />Demonstrated advantages to using PEEK<br />Proven use of CCD for DNA analysis<br />
Conclusion<br />New material and detection methods<br />Expanded applications for devices<br />Research shows promise for breaking limitations<br />
References<br />Ting-Wei Su, SungkyuSeo, Anthony Erlinger, AydoganOzcan, "High-Throughput Lensfree Imaging and Characterization of a Heterogeneous Cell Solution On a Chip", Biotechnology and Bioengineering 102 (2009) 856-868<br />Holger Muhlberger, Wonhee Hwang, Andreas E. Guber, Volker Saile, Werner Hoffmann, "Polymer Lab-on-a-Chip System with Electrical Detection", IEEE Sensors Journal, 8 (2008) 572-579<br />