Abstract

Z.M. Corales-Tirado, R.K. Rivera-Valentin, C. Rios-              University of Puerto Rico- Mayagüez
Velazquez
N...
whether the activity is due to the cloned fragment. The screening of detergent proteases for
industrial production has a g...
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Functional genomic and culture independent approaches to search for protease activity in Forest soils in Puerto Rico

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Transcript of "Functional genomic and culture independent approaches to search for protease activity in Forest soils in Puerto Rico"

  1. 1. Abstract Z.M. Corales-Tirado, R.K. Rivera-Valentin, C. Rios- University of Puerto Rico- Mayagüez Velazquez Names University Biology Biotechnology Department Category Text (Times New Roman, Font 12) Functional genomic and culture independent approaches to search for protease activity in Forest soils in Puerto Rico Z.M. Corales-Tirado, R.K. Rivera-Valentin, C. Rios-Velazquez, University of Puerto Rico- Mayagüez, Biology Department. The recognition that most microorganisms in the environment cannot be culture by standard methods stimulated the development of metagenomics, which is the genomic analysis of uncultured microorganisms. Modern biotechnology has a steadily increasing demand for novel bioactive compounds, biomolecules, and enzymes. Natural and genetic diversity of the soil metagenome has so far been the best supplier for these novel molecules. Enzymes production is one of the most important drivers of the industrial processing industry because it uses modern biology in the production process, and produces the biotechnological products needed for applications in other industrial areas. Some of these industrial enzymes are the proteases, which have important biotechnological applications and execute a large variety of functions. The proteases represent one of the three largest groups of industrial enzymes and have application in detergents, leather industry, food industry, pharmaceutical industry and bioremediation processes. Probably the largest application of proteases is in laundry detergents, where they help removing proteins based strains from clothing. Two approaches, the function-driven analysis and the sequence-driven analysis, have emerged to extract biological information from metagenomic libraries. Here, we used the function-driven analysis, which is initiated by identification of clones that express a desired trait, followed by characterization of the active clones by sequence and biochemical analysis. We screened three metagenomic libraries generated from forests in Puerto Rico. Serial dilutions of the libraries were spread on Petri plates supplemented with non-fat dry milk. A clear zone around the tested clones indicated clones with potential for protease activity. A total 20 potential clones were found which have being analyzed through biochemical and molecular techniques to confirm
  2. 2. whether the activity is due to the cloned fragment. The screening of detergent proteases for industrial production has a great importance because detergent enzymes account for about 30% of the total worldwide enzyme production, and represent one of the largest and most successful applications of modern industrial biotechnology.

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