IN VITRO
FERTILIZATION
    (IVF)
IN VITRO FERTILIZATION
STEPS:
 •Controlled Ovarian Stimulation
     (Lupron, Injectable Drugs)
 •Egg Retrieval from ovarie...
LUPRON:

 Taken either 10 days or 2 days before
 stimulation drugs. Stops early ovulation, can
 help grow more eggs. Given...
1. Wash hands thoroughly.



2. With new bottle, flip off
   plastic cover. Use alcohol
   swab; cleanse metal ring
   and...
4. Pull syringe
   plunger back until
   tip is at proper mark.

5. Uncover needle. Do not touch
   needle.



6. Place bo...
8. Pull back plunger
   until syringe fills to proper
   mark. Remove bubbles by pushing
   plunger up slowly. With needle...
10. Push plunger to inject
    medication. Withdraw needle.
    Wipe skin with alcohol swab.



11. Dispose of syringe.

 ...
Baseline Visit

•   Arrive with a full bladder
•   Estrogen
•   Follicular Ultrasound
•   Trial Transfer
•   Payment
•   S...
Monitor your Cycle using the
          PATIENT PORTAL
• Username and password

• View lab results and medication instructi...
GONAL F, FOLLISTIM, OR
BRAVELLE:
 Used for 7-12 days to grow eggs. Given just
 under skin with small needles. Potent drugs...
FOLLICLES NEAR TRIGGER
DAY:
OVIDREL:
 Given when eggs mature (several measure
 about 20 mm in size). This marks last day of
 Lupron and Follistim, Gon...
OVA ASPIRATION
35 HOURS LATER:
OOCYTE
OOCYTE & SPERM
ICSI
FERTILIZED OOCYTE
Medicines to start Day of
RETRIEVAL:

 Doxycycline: 1 tablet orally, twice daily, 5 days.
 Estrace: 1 tablet orally, twice...
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
LUTEAL PHASE SUPPORT
INTERMUSCULAR PROGESTERONE:
ASSISTED HATCHING:

  •A Laboratory Technique That ‘hatches’ an Area
     In Outer Coating of Embryo (Chemical vs. Laser)
...
Assisted Hatching
Pre-Implantation
Genetic Diagnosis
2, 3 or 5 Days after Retrieval:
         Embryo Transfer

Arrive with full or partially full bladder.
Bring Valium/Diaze...
Pregnancy Testing

• Approx 2 ½ weeks after Ovidrel shot
• Blood test for hCG in our office
• With a positive  ultrasound...
Wisconsin Fertility Institute: Injection Class 2009
Wisconsin Fertility Institute: Injection Class 2009
Wisconsin Fertility Institute: Injection Class 2009
Wisconsin Fertility Institute: Injection Class 2009
Wisconsin Fertility Institute: Injection Class 2009
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Wisconsin Fertility Institute: Injection Class 2009

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Wisconsin Fertility Institute presentation on In Vitro Fertilization.

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  • Do NOT go to this facility. I was in Last Monday as a new patient and I was appalled at how horribly The female doctor treats her nurses and staff. I heard from the female doctor's mouth many curse words, she caused one female nurse/tech to cry, and had nothing nice to say to her staff. I certainly don't want her helping me get pregnant. Then after research I find out she was fired/let go from UW for a frivolous sexual harassment suit towards another UW physician. DO NOT GO TO THIS CLINIC! It may look like a spa type environment but the female physician ( I believe her name is Dr. Pritts) is a horribly cruel and negative woman towards her co-workers. I will find another clinic to help conceive a child.
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Wisconsin Fertility Institute: Injection Class 2009

  1. 1. IN VITRO FERTILIZATION (IVF)
  2. 2. IN VITRO FERTILIZATION STEPS: •Controlled Ovarian Stimulation (Lupron, Injectable Drugs) •Egg Retrieval from ovaries •Fertilization In Vitro •Embryo Transfer to uterus
  3. 3. LUPRON: Taken either 10 days or 2 days before stimulation drugs. Stops early ovulation, can help grow more eggs. Given with small needle just under skin.
  4. 4. 1. Wash hands thoroughly. 2. With new bottle, flip off plastic cover. Use alcohol swab; cleanse metal ring and rubber stopper every day, just before use. 3. Remove outer wrapping from one syringe.
  5. 5. 4. Pull syringe plunger back until tip is at proper mark. 5. Uncover needle. Do not touch needle. 6. Place bottle on flat surface, push needle through rubber stopper. Push plunger to inject air into bottle. 7. Keep needle in bottle. Lift bottle, turn straight upside down. Check to see that needle tip is in liquid.
  6. 6. 8. Pull back plunger until syringe fills to proper mark. Remove bubbles by pushing plunger up slowly. With needle tip still in liquid, pull the plunger until at proper mark. 9. Cleanse injection site with alcohol swab. Stretch skin.Slide needle through skin, into subcutaneous tissue, at 90 angle.
  7. 7. 10. Push plunger to inject medication. Withdraw needle. Wipe skin with alcohol swab. 11. Dispose of syringe. Remember! Use disposable syringe only once
  8. 8. Baseline Visit • Arrive with a full bladder • Estrogen • Follicular Ultrasound • Trial Transfer • Payment • Sign Consent
  9. 9. Monitor your Cycle using the PATIENT PORTAL • Username and password • View lab results and medication instructions • Results are ready by 1PM each day • Schedule next appointment with front desk
  10. 10. GONAL F, FOLLISTIM, OR BRAVELLE: Used for 7-12 days to grow eggs. Given just under skin with small needles. Potent drugs, so monitoring with ultrasound and blood draws and potential changes in dosing will occur.
  11. 11. FOLLICLES NEAR TRIGGER DAY:
  12. 12. OVIDREL: Given when eggs mature (several measure about 20 mm in size). This marks last day of Lupron and Follistim, Gonal F or Bravelle. Given just under skin with small needle. IMPORTANT TO TAKE THIS ONE AT EXACTLY CORRECT TIME!
  13. 13. OVA ASPIRATION 35 HOURS LATER:
  14. 14. OOCYTE
  15. 15. OOCYTE & SPERM
  16. 16. ICSI
  17. 17. FERTILIZED OOCYTE
  18. 18. Medicines to start Day of RETRIEVAL: Doxycycline: 1 tablet orally, twice daily, 5 days. Estrace: 1 tablet orally, twice daily* Progesterone: 1mL shot same time daily* * Continued until 11 weeks gestation
  19. 19. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  20. 20. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  21. 21. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  22. 22. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  23. 23. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  24. 24. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  25. 25. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  26. 26. LUTEAL PHASE SUPPORT INTERMUSCULAR PROGESTERONE:
  27. 27. ASSISTED HATCHING: •A Laboratory Technique That ‘hatches’ an Area In Outer Coating of Embryo (Chemical vs. Laser) •May Improve Implantation •Indications: Age 38 or greater Prior Failed IVF Cycles ICSI Embryos (Intracytoplasmic Sperm Injection)
  28. 28. Assisted Hatching
  29. 29. Pre-Implantation Genetic Diagnosis
  30. 30. 2, 3 or 5 Days after Retrieval: Embryo Transfer Arrive with full or partially full bladder. Bring Valium/Diazepam tablet. Transfer performed, 30 minutes of rest. Low activity for next few days. Bedrest not recommended. Normal activity within 3 days.
  31. 31. Pregnancy Testing • Approx 2 ½ weeks after Ovidrel shot • Blood test for hCG in our office • With a positive  ultrasound 3 weeks later • With bleeding or other problems  repeat hCG • With a negative  follow up with physician
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