Cladman wendy cv and addendum 2012


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Wendy Cladman CV and skills addendum

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Cladman wendy cv and addendum 2012

  1. 1. WendyCLADMANScientific Research ProfessionalExperimental Design and Execution 519-268-0649Method Development Management wcladman@sympatico.caCareer SummaryA skilled and accomplished laboratory professional with extensive experience conducting/analyzingprotocols and experiments. Expertise in laboratory management, equipment usage and maintenance,instruction of personnel, writing reports and contributing to publications. A talent for analyzing andresolving complex problems, developing and simplifying procedures. Works well as part of a team orindependently. Ability to adapt to different situations.Critical Skill Set Biochemical assays  Data analysis  Cell culture Spectrophotometry  Laboratory management  Microbiology techniques Immunoprecipitations  Complex problem solving  Protein purification Liquid Chromatography  Computer skills  DNA and RNA extractions Molecular biology  Recombinant DNA protocols  Immunoblotting Preparation of reports  Gene expression  Radioactive assaysProfessional Employment History University of Western Ontario, Department of Biology, Dr. Stan Caveney Laboratory Technician level 5 January 2005 – July 2008 Area of Research: Monoamine neurotransmitter transporters in invertebrates  Conducted phylogenetic study of neurotransmitter transporters in invertebrates and vertebrates using RT PCR, BLAST searches and genome banks, contributing to a publication.  Designed experiments to study the effects of kinases on the uptake and efflux functions of the octopamine transporter in a live cell assay.  Examined the functional role of neurotransmitter transporters in a moth by RNA interference and quantitative PCR. University of Western Ontario, Department of Physiology and Pharmacology, Dr. Peter Chidiac Laboratory Technician level 5 October 1998 - November 2004 Area of Research: Regulation of G-proteins by receptors and RGS proteins  Developed a new steady state GTPase assay and improved purification methods for RGS proteins and G-proteins.  Analyzed the function of several RGS proteins through a series of complex assays and procedures.  Set up and managed laboratory. Trained and supervised personnel and students in laboratory protocols. University of Guelph, Department of Food Science, Dr. Mansel Griffiths Research Associate April 1997 - February 1998 Area of Research: Develop methods of detection of food pathogens, milk trials  Collected, purified and classified bacteriophage specific for food pathogenic bacteria for the detection of pathogens in food and food processing equipment.  Performed milk trials to test and compare efficacy of polyethylene terephthalate (PET) milk containers resulting in a publication. Page 1
  2. 2. Wendy Cladman CVUniversity of Guelph, Department of Chemistry and Biochemistry, Dr. Alan Mellors Research Assistant February 1991 - January 1997 Area of Research: Study of O-sialoglycoprotein endopeptidase and other proteins secreted by P. haemolytica  Developed better methods to purify enzymes secreted by bacteria causing shipping fever in cattle.  Designed an assay to accurately quantify the degree of substrate cleavage by the glycoprotease of interest.  Determined the efficacy of antibodies to be used in vaccine trials.Contract Positions:Diagnostics Biochem Canada, Dorchester, Ontario Production of ELISA assay kits June 2012 - August 2012University of Western Ontario, Department of Microbiology and Immunology, Dr. Miguel Valvano Laboratory Technician level 5 February 2010 - June 2010 Area of Research: Lipopolysaccharide mechanism of assembly in infectious bacteriaUniversity of Western Ontario, Department of Biology, Dr. John Wiebe Laboratory Technician level 5 September 2008 – March 2009 Area of Research: Purification of progesterone metabolite receptor in breast cancer cellsLimagrain Canada Seeds Incorporated, Listowel, Ontario Laboratory technician in a canola research station April 1998 – August 1998 Area of Research: Canola Research StationMusic Theater:Pfalztheater Kaiserslautern and Vereinigten Städtische Bühnen Krefeld-Mönchengladbach,Germany Opera Singer September 1979 - August 1990  Rehearsed and performed operas, operettas and musicals on stage, performed in concerts.Education and Training Graduated with a Bachelor of Science Degree in the biological sciences from York University, Toronto, Ontario, Canada Additional Courses (audited and/or participated in) University of Toronto Medical Genetics 430H Recombinant DNA Methods – laboratory course University of Guelph Biochemistry 19-258 Introductory Biochemistry Food Science 42-323 Food Microbiology – laboratory course University of Western Ontario Pharmacology 462 Advanced Principles of Pharmacology Pharmacology 443 Pharmacology and Toxicology of Natural-Sourced Medicine Pharmacology 355b Principles of Drug Action Attended Great Lakes GPCR Retreat in 2000, 2002 and 2003 Other laboratory training:  WHMIS Training, University of Western Ontario, Agriculture Canada  Biohazard Safety, University of Western Ontario  Radiation Safety Training, University of Western Ontario, Agriculture Canada  UWO Financials Training - Electronic Purchase Requisitions and AP/PO Inquiries, University of Western Ontario, 2010  Laboratory and Environmental Waste Management Safety Workshop, University of Western Ontario, 1998 and 2010  Frequently attended seminars, equipment demonstrations, equipment training sessions and trade shows. Page 2
  3. 3. Wendy Cladman CVPublicationsPapers published in refereed journalsDonly, C.,Verellen, L., Cladman, W., Caveney, S. 2007. Functional comparison of full-length and N- terminal-truncated octopamine transporters from Lepidoptera. Insect Biochemistry and Molecular Biology, 37(9):933-940.Caveney, S., Cladman, W., Verellen, L. and Donly, C. 2006. Ancestry of neuronal monoamine transporters in the metazoan. The Journal of Experimental Biology 209: 4858-4868.Hepler, J.R., Cladman, W., Ramineni, S., Hollinger, S. and Chidiac, P. 2005. A novel activity of RGS14 on Goα and Gi2α nucleotide binding and hydrolysis independent of its RGS domain and GDI activity. Biochemistry, 44(14):5495-5502.Bernstein, L.S., Ramineni, S., Hague, C., Cladman, W., Chidiac, P., Levey, A.I., and Hepler, J.R. 2004. RGS2 binds directly and selectively to the M1 muscarinic acetylcholine receptor third intracellular loop to modulate Gq/11α signaling. The Journal of Biological Chemistry 279:21248-21256.Cladman, W.M. and Chidiac, P. 2002. Characterization and comparison of RGS2 and RGS4 as GTPase activating proteins for M2 muscarinic receptor-stimulated Gi. Molecular Pharmacology 62:654-659.Cladman, W.M., Scheffer, S., Goodrich, N. and Griffiths, M.W. 1998. Shelf life of milk packaged in plastic containers with and without treatment to reduce light transmission. International Dairy Journal. 8:629-636.Cladman, W.M., Watt, M.-A.V., Dini, J.-P. and Mellors, A. 1996. The Pasteurella haemolytica O-sialoglycoprotein endopeptidase is inhibited by zinc ions and does not cleave fetuin. Biochemical and Biophysical Research Communications. 220:141-146.Lee, C.W., Shewen, P.E., Cladman, W.M., Conlon, J.A.R., Mellors, A. and Lo, R.Y.C. 1994. Sialoglycoprotease of Pasteurella haemolytica A1: Detection of antisialoglycoprotease antibodies in sera of calves. Canadian Journal of Veterinary Research. 58: 93-98.Published AbstractsCaveney, S., Cladman, W.,Verellen, L., Donly, C. 2005. Origin and evolutionary history of metazoanmonoamine transporters. 15th Neuropharmacology Conference, New Perspectives inNeurotransmitter Transporter Biology, Washington DC, USA, Abstract no. P68.Desjardins, R.L., Cladman, W. and Chidiac, P. 2003. Evidence for stimulus trafficking at the M2 muscarinic receptor. Great Lakes GPCR Retreat, Honey Harbour, Ontario.Zhao, Q., Cladman, W. and Chidiac, P. 2003. Receptor-dependent differences between RGS4 and RGS5 as GTPase activating proteins for Go and Gi2. Great Lakes GPCR Retreat, Honey Harbour, Ontario.Roy, A.A., Cladman, W.M., Lemberg, K.E., Ferguson, S.S., Cook, M.A. and Chidiac, P. 2001. G protein selectivity of RGS2 and RGS4. Society for Neuroscience 31st Annual Meeting, San Diego CA. USA, Abstract no. 43.12.Roy, A.A., Cladman, W.M., Lemberg, K.E. and Chidiac, P. 2001. Effects of Gi, Gq and Gs on the intracellular localization of GFP-RGS2 and GPF-RGS4. Great Lakes GPCR Retreat, Bromont, Quebec.Cladman, W. and Chidiac, P. 2000. Stimulation of Gi GTPase activity by RGS2 and RGS4 in a membrane-based assay system. Great Lakes GPCR Retreat, London, Ontario.Mellors, A., Watt, M.-A., Shad, Y., Cladman, W.M., and Lo, R.Y.C. 1994. The O- sialoglycoprotease endopeptidase of Pasteurella haemolytica. Canadian Federation of Biological Science, Montreal. Page 3
  4. 4. Wendy Cladman CV AddendumLaboratory SkillsBiochemical and enzymatic assays  Assaying for proteins and enzymatic activity by UV-VIS spectrophotometric and fluorometric procedures as well as by densitometry, autoradiography and radioactive incorporation  Milk quality testing: protein, lipid and Vitamin A degradation, measurement of bacterial contamination  Vitamin and fatty acid extractions and quantitative analysis by HPLC and GC  Immunoprecipitations, immunoblotting techniques (colorimetric and chemiluminescent), ELISA assays, radioimmuno assays (RIA), electrophoresis, whole cell electroelution  Steady state assays, G protein and/or receptor radioligand binding, densitometry, substrate degradation assays, complex time course experiments, live cell uptake and efflux assays,Microbiology and cell biology  Maintaining mammalian and insect cell culture and baculovirus stocks, microscopy, cabbage looper moth rearing  Maintaining bacterial strains and isolating mutants, aerobic and anaerobic  Making bacteriophage or virus lysates by infection or induction, purifying phage and phage DNA (single and double stranded) by CsCl gradient ultracentrifugation  Screening and classification of pathogenic bacteria by staining, catalase and oxidase tests, biochemical screening media and API stripsMolecular biology  Recombinant DNA techniques, RT PCR, quantitative PCR, RNA interference, RACE, gene isolations, ligations, cloning, bioinformatics  RNA extractions and purifications from invertebrate tissue samples, restriction enzyme digestions, reverse transcriptions, DNA extractions, plasmid kits  Transformation, induction and expression of cloned genes, denaturation and renaturation of purified proteinsGene expression and protein purification  Purification and analysis of membrane-bound proteins, enzymes and glycoproteins by precipitations; column chromatography with HPLC, HRLC, FPLC, low pressure, batch and gravity systems utilizing ion exchange, affinity, adsorption and size exclusion resins; differential centrifugation  Preparation of crude extracts from bacterial cultures and supernatants, mammalian tissues, mammalian and insect cell cultures by various methods (sonication, French Pressure cell, glass bead homogenization, polytron, osmotic shock and enzymatic lysis)Data analysis and computer skills  Kinetic analysis and statistical programs (Anova, Origin, Prism, Sigma Plot)  Sequence analysis: DNA Star, Oligo, Gene Bank, BLAST search  Spread sheets and plots (Excel, Origin), word processing  Quantification of purified proteins and immunoblot data, analysis of densitometric dataLaboratory management  Maintenance and documentation of chemical stocks, lab supplies, data bases and resource collections  Maintenance of equipment and software; training of personnel in the use of equipment  Assisting with the preparation of grant applications, reports and research publications  Radioactive inventory and waste disposal  Setup and organization of facilities, organization of meetings and schedulesUse and maintenance of laboratory equipment  FPLC, HPLC, HRLC, GC, electroporator, Thermal cycler (PCR), Light cycler, Rotor Gene  Liquid scintillation counter, Ultracentrifuge, High-speed centrifuge, electrophoresis  Fluorchem imager, phospho-imager, spectrophotometer, fluorometer, lyophilizer Page 4