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Overview DNA assays to study and manage soilborne diseases

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Alan McKay speaking at the Mannum Community Science Forum

Alan McKay speaking at the Mannum Community Science Forum

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  • The interaction is not significant between (Fungicide x depth x time). The interaction between depth and time is significant (
  • Statistically significant at treatment x depth interaction (p=.009), the main effects of treatment and depth are also significant.
  • Transcript

    • 1. South Australian Murray Darling Basin NRM Board Ranges to River NRM - Community Science Forum   Farming Sustainability & Technologies  
    • 2. Overview DNA assays to study and manage soilborne diseases Alan McKay & Kathy Ophel Keller Peter Blacket, 2009
    • 3.  
    • 4. Cost of soilborne diseases in Wheat Murray & Brennan, 2009 Disease Cost AUS$m Contribution to control AUS$m Potential Current Breeding Cultural Pesticide Cereal cyst nematode 572 58 377 137 0 Crown rot 434 79 83 272 0 Pratylenchus neglectus 266 73 48 146 0 P. thornei 167 50 60 57 0 Rhizoctonia 165 59 0 100 6 Take-all 109 13 0 86 10 Common root rot 108 30 46 32 0 Pythium 28 10 0 17 1 TOTAL 1,849 372 614 847 17
    • 5. PreDicta B - SARDI service to monitor soilborne pathogen levels
      • Cereal Cyst Nematode
      • Take-all (wheat strain)
      • Take-all (oat strain)
      • Rhizoctonia (AG8)
      • P. neglectus
      • P. thornei
      • Crown rot (3 tests)
      • Blackspot (peas-2 tests)
    • 6. Amplification Plot DNA Amplification Plot
    • 7. Gaeumannomyces graminis var tritici + avenae (Take-all) levels before seeding 2010
    • 8. Rhizoctonia solani AG8 (bare patch) levels before seeding 2010
    • 9. Pratylenchus neglectus (root lesion nematode) levels before seeding 2010
    • 10. Pratylenchus thornei (root lesion nematode) levels before seeding 2010
    • 11. Fusarium pseudograminearum (crown rot) levels before seeding 2010
    • 12. Cereal cyst nematode levels before seeding 2010
    • 13. CCN resistance: Preliminary results SA 2010
    • 14. Rhizoctonia bare patch
    • 15. Uneven growth
    • 16. Vivien Vanstone DAFWA Symptoms Rhizoctonia Pratylenchus
    • 17.  
    • 18. No-Till wheat Wheat Cultivation Grass Free Pasture Canola Sown to Wheat Impact of summer rain and rotation Gupta, 2010
    • 19. Rhizoctonia levels in soil at seeding (3 June 2010) 0-10= 85 pg DNA / g soil
    • 20. In-row Rhizoctonia DNA levels 5 wks after sowing 8 – 9 July 2010 Interaction – Treatment x depth, P val = 0.03, l.s.d. (0.05) = 103 Knife Blade Coulter Disc Seeding level Untreated Treated Untreated Treated Untreated Treated Untreated Treated
    • 21. 8 wks after seeding Treated Untreated
    • 22. Sjaan Davey Treated Untreated
    • 23. Coulter in-row, Rhizoctonia DNA levels 5 and 10 weeks after sowing in Untreated and treated plots Rhizoctonia DNA concentrations increase with time Rhizoctonia DNA concentrations in 0-5cm lower in fungicide treated plots
    • 24. Wheat root response in row 10 weeks after sowing Wheat DNA concentrations in 0-5 cm higher in fungicide plots
    • 25. Other Project Areas
      • Evaluating resistance and tolerance to cereal nematodes
      • Controlling Rhizoctonia
      • Potatoes - Developing a testing service for soilborne pathogens
      • Soil health – developing assays to assess community structure of key free living nematodes
      • Root knot nematodes in vegetables
      • Root architecture of P efficient varieties
    • 26. Acknowledgements Financial support Scientists Organisation Diagnostics Staff: Herdina, Ina Dumitrescu, Jan Gooden, Russell Burns, Aidan Thomson, Paul Webber, Ian Riley. SARDI Di Hartley CSIRO Gupta Vadakattu CSIRO Jack Desbiolles UniSA Sjaan Davey SARDI Dan Smith SARDI Ray Correll Rho Environmetrics David Roget Ex CSIRO

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