Isolation and Transmission Electron
    Microscopic Characterization of
Bacteriophages in Sewage Specific for
E.coli O157:...
My Question?
• Can I prove that phages are present in the
  sample collected from a sewage plant by
  visualizing them via...
Philips CM 100 Transmission Electron
            Microscope
E.Coli O157:H7 phage from my sample




               A   B




               C   D
Citrobacterphage from my sample




             A   B




             C   D
Conclusion
• Water samples from sewage plants are rich in
  phages forE. coli O157:H7 and
  Citrobacterfreundii as it was ...
Acknowledgements
•   Dr. Kathleen Bobbitt
•   Prof. Christopher Corbo
•   Zulmaire Franco
•   Dr. Roy Mosher
•   Dr. Adam ...
Salvatore Valenti
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Salvatore Valenti

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Salvatore Valenti, a graduating senior at Wagner College, used this Power Point slideshow in delivering his senior thesis presentation.

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Transcript of "Salvatore Valenti"

  1. 1. Isolation and Transmission Electron Microscopic Characterization of Bacteriophages in Sewage Specific for E.coli O157:H7 and CitrobacterFreundii Salvatore Valenti-Microbiology Department of Biological Sciences
  2. 2. My Question? • Can I prove that phages are present in the sample collected from a sewage plant by visualizing them via Transmission Electron Microscopy? • By answering this question I will help future generations of Wagner microbiology students perform further analyses of bacteriophages.
  3. 3. Philips CM 100 Transmission Electron Microscope
  4. 4. E.Coli O157:H7 phage from my sample A B C D
  5. 5. Citrobacterphage from my sample A B C D
  6. 6. Conclusion • Water samples from sewage plants are rich in phages forE. coli O157:H7 and Citrobacterfreundii as it was proven with electron microscopy. • Having learned the techniques to isolate bacteriophages and visualize them with the electron microscope makes me more competitive in the job market.
  7. 7. Acknowledgements • Dr. Kathleen Bobbitt • Prof. Christopher Corbo • Zulmaire Franco • Dr. Roy Mosher • Dr. Adam Houlihan
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