CELLULAR EVENTS IN ZEBRAFISH OPTIC TECTAL BRAIN EXPLANTS: A MODEL TO ANALYZE NEUROTRAUMA AND NEUROREGENERATION  by Michael...
Research Focuses in the Laboratory<br />To study cellular events taking place in nervous tissue in cases of Traumatic Brai...
Zebrafish (Daniorerio) <br />
Zebrafish Brain <br />
Culture Conditions<br />
Light microscopic analysis showed formation of embryoid structures as early as 48 hours in culture which further different...
My Personal Research Goals<br />Scanning electron microscopy <br />Confocal laser scanning microscopy <br />BrdU Cell Prol...
Scanning Electron MicroscopyTop Row – 12 Hours of cultivationBottom Row – 24 Hours of Cultivation <br />
Scanning Electron Microscopy48 Hours of Cultivation  <br />
Scanning Electron Microscopy96 Hours of Cultivation <br />
Zebrafish Brain Cytoarchitecture seen with Immunohistochemistry and Confocal Microscopy <br />
BrdU Assay for Cell Proliferation6 & 48 Hours of Cultivation<br />6 h<br />48 h<br />
BrdU Assay for Cell Proliferation96 Hours of Cultivation<br />
BrdU Assay for Cell ProliferationPositive Control: CHO Cells<br />
Conclusion<br />Adult zebrafish brain demonstrates regenerative capacities in surviving organotypic culture. <br />This re...
Acknowledgements <br />Professor Christopher Corbo<br />Dr. Alejandra del C. Alonso <br />Dr. William L’Amoreaux<br />Dr. ...
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Michael Gutkin (Biology)

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Michael Gutkin, a 2010 graduating senior at Wagner College (B.S. in Biology), uses this Power Point slideshow to help make his thesis presentation.

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Michael Gutkin (Biology)

  1. 1. CELLULAR EVENTS IN ZEBRAFISH OPTIC TECTAL BRAIN EXPLANTS: A MODEL TO ANALYZE NEUROTRAUMA AND NEUROREGENERATION  by Michael C. GutkinReflective Tutorial in Biology(Senior Learning Community) Experiential Component: ResearchDepartment of Biological SciencesWagner College Spring, 2010<br />
  2. 2. Research Focuses in the Laboratory<br />To study cellular events taking place in nervous tissue in cases of Traumatic Brain Injury (TBI).<br />To use a model animal whose brain has known regenerative capacities. <br />To use a model animal which inexpensive and easy to handle. <br />
  3. 3. Zebrafish (Daniorerio) <br />
  4. 4. Zebrafish Brain <br />
  5. 5. Culture Conditions<br />
  6. 6. Light microscopic analysis showed formation of embryoid structures as early as 48 hours in culture which further differentiated with the extended time in culture. This picture is from a sample cultivated for 96 hours.<br />
  7. 7. My Personal Research Goals<br />Scanning electron microscopy <br />Confocal laser scanning microscopy <br />BrdU Cell Proliferation<br />Immunohistochemistry<br />
  8. 8. Scanning Electron MicroscopyTop Row – 12 Hours of cultivationBottom Row – 24 Hours of Cultivation <br />
  9. 9. Scanning Electron Microscopy48 Hours of Cultivation <br />
  10. 10. Scanning Electron Microscopy96 Hours of Cultivation <br />
  11. 11. Zebrafish Brain Cytoarchitecture seen with Immunohistochemistry and Confocal Microscopy <br />
  12. 12. BrdU Assay for Cell Proliferation6 & 48 Hours of Cultivation<br />6 h<br />48 h<br />
  13. 13. BrdU Assay for Cell Proliferation96 Hours of Cultivation<br />
  14. 14. BrdU Assay for Cell ProliferationPositive Control: CHO Cells<br />
  15. 15. Conclusion<br />Adult zebrafish brain demonstrates regenerative capacities in surviving organotypic culture. <br />This regeneration is characterized in SEM by relocation of “stem-like cells” and the formation of embryoid bodies accompanied by neovascularization within spongiform degenerative regions. <br />Vital cells can be detected by IHC as well as cell proliferation, but dividing cells are seen less than expected. <br />
  16. 16. Acknowledgements <br />Professor Christopher Corbo<br />Dr. Alejandra del C. Alonso <br />Dr. William L’Amoreaux<br />Dr. ZoltanFulop<br />Professor Linda Raths<br />

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