Your SlideShare is downloading. ×
Presentación biología molecular
Upcoming SlideShare
Loading in...5
×

Thanks for flagging this SlideShare!

Oops! An error has occurred.

×
Saving this for later? Get the SlideShare app to save on your phone or tablet. Read anywhere, anytime – even offline.
Text the download link to your phone
Standard text messaging rates apply

Presentación biología molecular

43
views

Published on

Published in: Health & Medicine, Technology

0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
43
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
1
Comments
0
Likes
0
Embeds 0
No embeds

Report content
Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
No notes for slide

Transcript

  • 1. Importin β-dependent nuclear import of TopBP1 in ATR–Chk1 checkpoint in Xenopus egg extracts and Evolution of a cellular immune response in Drosophila: a phenotypic and genomic comparative analysis. Virginia Espinosa Navarro Medicine student III Semester UPB
  • 2. BIBLIOGRAPHY MEDICAL UTILITY This first study is of great clinical importance because, genetic and molecular biological studies offer us a path toward personalized treatment and prognostic factors, one of which includes the prevention of breast cancer, and it is known that family susceptibility to breast cancer comprises 25% of all breast tumors, so the ratio of the TopBP1 protein susceptibility to breast cancer is a step that leads us to find an appropriate and effective prevention. In the second study, the clinical importance of the phenotypic and genomic analysis comes from the possibility that the drug therapy can be safer and more effective when the genotype of the patient is known. Pharmacogenetic studies can be considered studies of interaction between genes and environment, considering pharmacological treatment as an environmental variable. • • Importin β-dependent nuclear import of TopBP1 in ATR–Chk1 checkpoint in Xenopus egg extracts. January 9, 2014. From: www.elsevier.com/locate/cell sig. Evolution of a cellular immune response in Drosophila: a phenotypic and genomic comparative analysis. Janyary 18, 2014. From: http://gbe.oxfordjournals.org /. Importin β-dependent nuclear import of TopBP1 in ATR–Chk1 checkpoint in Xenopus egg extracts and Evolution of a cellular immune response in Drosophila: a phenotypic and genomic comparative analysis. Virginia Espinosa Navarro Medical student III semester UPB Teacher Lina Martínez
  • 3. INTRODUCTION In the first article, we seek to clarify one of the aspects that influence the susceptibility to breast cancer, and it’s importing the TopBP1 protein from the nucleus to the cytoplasm. Taking advantage of the cell-free Xenopus egg extract system, was investigated the roles of TopBP1 in DNA metabolism including DNA replication and DDR through a series of studies And all these data suggest that the Importin β-dependent shuttling of TopBP1 into nucleus plays an important role in the ATR–Chk1 checkpoint signaling in Xenopus. At the second one, the propose that they had with the article was investigate the large interspecific differences in the ability to survive infection by parasitoids, across 11 Drosophila species and identify genomic changes associated with the evolution of cellular immunity in the Drosophila genu and the gains and losses of parasitoid resistance. Importin β-dependent nuclear import of TopBP1 in ATR–Chk1 checkpoint in Xenopus egg extracts By identifying the importance of having the TopBP1 protein in DNA metabolism, replication, damage response and transcriptional regulation, and besides that, it has been identified that its location is associated with susceptibility to breast cancer , they wanted to do this study to investigate the way this core protein is transported to the cytoplasm, and a protein that interacted with TopBP1 C-terminal, known as B importin was found. After a series of procedures, managed to conclude important facts such as: Importin β has been identified as a new interacting protein of TopBP1 C-terminus. •A C-terminal motif in TopBP1 is important for its nuclear import. •Importazole inhibits TopBP1's nuclear import and ATR–Chk1 checkpoint signaling. •With ongoing DNA replication, nuclear import of TopBP1 is required for checkpoint. •Checkpoint activation requires more TopBP1 than DNA replication does. Evolution of a cellular immune response in Drosophila: a phenotypic and genomic comparative analysis As well the name says , this article seeks to evaluate the evolution of the immune response by phenotypic and genotypic comparison, for that, was investigated the large interspecific differences in the ability to survive infection by parasitoids across 11 Drosophila species and identify genomic changes associated with gains and losses of parasitoid resistance. They provide an important step towards understanding the evolution of the cellular resistance against parasitoids in Drosophila species and they showed that a combination of cooption and neofunctionalization is likely to have contributed to the acquiring of the new immunity component in the cellular immune response, and that particular gene families (serine-type proteases, Teps and lectins ) could be of special interest for the processes of hemocyte differentiation, proliferation and activation.
  • 4. The susceptibility to breast cancer comprises 25% of all breast tumors, and the importing the TopBP1 protein from the nucleus to the cytoplasm plays an important role in this. Taking advantage of the cell-free Xenopus egg extract system, was investigated the roles of TopBP1 in DNA metabolism including DNA replication and DDR through a series of studies And all these data suggest that the Importin β-dependent shuttling of TopBP1 into nucleus plays an important role in the ATR– Chk1 checkpoint signaling in Xenopus. The study about the evolution of a cellular immune response in Drosophila, show us that through a combination of phenotypic and genomic characterizations , they provide an important step towards understanding the evolution of the cellular resistance against parasitoids in Drosophila species. .
  • 5. MATERIALS AND METHODS Xenopus egg extract preparation and related procedures Recombinant proteins and antibodies Coimmunoprecipitation (Co-IP) assays Immunofluorescence microscopy GST pulldown assays
  • 6. RESULTS Importin β interacts with TopBP1 The CTM motif of TopBP1 mediates its interaction with Importin β The CTM motif of TopBP1 is required for nuclear import from outside of assembled nuclei. The CT100 fragment of Importin β is required for TopBP1 association
  • 7. RESULTS Importin β-specific inhibitor importazole compromised the nuclear import of TopBP1 Nuclear import of TopBP1 plays a distinct regulatory role in the DNA damage response The CTM-dependent nuclear import of TopBP1 is required for ATR–Chk1 checkpoint signaling, not due to its requirement for DNA replication
  • 8. OBSERVATION Breast cancer is a reality that affects all women, and all of which are likely to have, it is vital to attend these studies, where molecular biology gives us significant events that influence the disease. In my opinion it is an article of great interest and it brings a lot to the medical science, indicating the influence of the TopBP1 protein in breast cancer susceptibility.
  • 9. MATERIALS AND METHODS Species strains Encapsulation assay Melanization Candidate genes Lamellocyte identification
  • 10. MATERIALS AND METHODS Orthologous groups and homology categories Phylogenetic analysis Immune classification Positive selection RT-qPCR
  • 11. RESULTS Phenotypic characterization: Only species of the melanogaster subgroup show encapsulation ability and produce lamellocytes Comparative Genomics Orthologs Comparative expression of TepI, PPO3 and IM1 Lineage specic gains and losses Sequence divergence
  • 12. OBSERVATION In this studio, they provide an important step towards understanding the evolution of the cellular resistance against parasitoids in Drosophila species, that’s important because, It has been argued that it may be beneficial to the public health use genetic information to stratify the allocation of environmental settings that prevent disease, although this view is not universally accepted.
  • 13. MEDICAL UTILITY This first study is of great clinical importance because, genetic and molecular biological studies offer us a path toward personalized treatment and prognostic factors, one of which includes the prevention of breast cancer,.
  • 14. MEDICAL UTILITY it is known that family susceptibility to breast cancer comprises 25% of all breast tumors, so the ratio of the TopBP1 protein susceptibility to breast cancer is a step that leads us to find an appropriate and effective prevention.
  • 15. MEDICAL UTILITY In the second study, the clinical importance of the phenotypic and genomic analysis comes from the possibility that the drug therapy can be safer and more effective when the genotype of the patient is known. Pharmacogenetic studies can be considered studies of interaction between genes and environment, considering pharmacological treatment as an environmental variable
  • 16. BIBLIOGRAPHY • Importin β-dependent nuclear import of TopBP1 in ATR–Chk1 checkpoint in Xenopus egg extracts. January 9, 2014. From: www.elsevier.com/locate/cellsig. • Evolution of a cellular immune response in Drosophila: a phenotypic and genomic comparative analysis. Janyary 18, 2014. From: http://gbe.oxfordjournals.org/.