Dr.usrinivasa,m.Pharm,Ph.D
GeneTransfer in Plants
• Genetic transfer involves the removal of
gene from one species and inserting them
into another. This does not require se...
• This transgenic plants carry this stable
integrated genes thus those plants obtained
through genetic engineering contain...
• Gene transfer requires transgene and
targeted cell.
i. Transgene : isolated genes of plants
viruses , bacteria etc.
ii. ...
• Overall process involves the following
stages for producing transgenic plant.
1. Gene transfer
2. Selection of transgeni...
There are two methods
1.Vector mediated methods
2.DNA mediated gene transfer methods
Classification of gene transfer method
• Vectors are DNA carriers into which the genes are
inserted to make recombinant DNA
• Vectors along with this foreign gen...
(A)Using Agrobacterium tumefaciens :-
• It is Gram-negative bacterium , commonly
occuring in soil. It Causes “ Crown gall
...
 Process of Gene Transfer :-
• By genetic transfer , the T1 Plasmids of
this bacterium can be modified to create
“Disarme...
• These vectors can carry any DNA
sequence into
A rumefaciens infected plant without
causing tumors growth .
• By placing ...
• Cloning of the required foreign DNA
sequence into vector using E-coli host is
usually practiced.
• As plasmid resides in...
Introduction of ADA-gene
i. Improvement in both quality and quantity (yield) of crop.
ii. Development of herbicidal resistance
iii. Development of ...
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Gene transfer in plants by Dr.Srinivasa, Professor and Head, Srinivas college of pharmacy, Mangalore

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Gene transfer in plants by Dr.Srinivasa, Professor and Head, Srinivas college of pharmacy, Mangalore

  1. 1. Dr.usrinivasa,m.Pharm,Ph.D GeneTransfer in Plants
  2. 2. • Genetic transfer involves the removal of gene from one species and inserting them into another. This does not require sexual incompatibility . • The new DNA remains stable and can transmit the transferred gene to offspring , resulting in development of transgenic plants.
  3. 3. • This transgenic plants carry this stable integrated genes thus those plants obtained through genetic engineering contain a gene from and unrelated organ such genes are called trans gene and those plants contain them are called transgenic plants. • The technique of introduction of foreign gene is called genetic transformation .
  4. 4. • Gene transfer requires transgene and targeted cell. i. Transgene : isolated genes of plants viruses , bacteria etc. ii. Targeted cells: culture cells ,protoplast ,pollens, zygots, and also cells from immature cells
  5. 5. • Overall process involves the following stages for producing transgenic plant. 1. Gene transfer 2. Selection of transgenic plant 3. The recovery of transgenic plant
  6. 6. There are two methods 1.Vector mediated methods 2.DNA mediated gene transfer methods Classification of gene transfer method
  7. 7. • Vectors are DNA carriers into which the genes are inserted to make recombinant DNA • Vectors along with this foreign genes are introduce into appropriate host cells ,vectors are two types 1. Cloning vectors: This are use for making millions of DNA segments 2. Expression vectors: This are use for expression of clone gene to produce product Vector mediated methods:
  8. 8. (A)Using Agrobacterium tumefaciens :- • It is Gram-negative bacterium , commonly occuring in soil. It Causes “ Crown gall disease ”. • On entering into plant through mechanically wounded tissue site , it causes tumerous outgrowth due to proliferation of undifferentiated tissue .
  9. 9.  Process of Gene Transfer :- • By genetic transfer , the T1 Plasmids of this bacterium can be modified to create “Disarmed Plasmids ” , known as Vectors .
  10. 10. • These vectors can carry any DNA sequence into A rumefaciens infected plant without causing tumors growth . • By placing foreign gene into T-DNA region of the T1 plasmid , cloning of the introduced gene is possible .
  11. 11. • Cloning of the required foreign DNA sequence into vector using E-coli host is usually practiced. • As plasmid resides inside bacteria , growing them on a medium leads to simultaneous multiplication of bacteria and plasmid
  12. 12. Introduction of ADA-gene
  13. 13. i. Improvement in both quality and quantity (yield) of crop. ii. Development of herbicidal resistance iii. Development of pesticidal resistance iv. To produce valuable products - Antigen and antibody production - Vaccine production - Oligopeptide and protein production - Production of cyclodextrins - Enzymes like anylase APPLICATION OF GENETRANSFER::

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