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Writing a Systematic Reviewa good start of a PhD-project           Marije SloffPhD-student Experimental Urology       Tiss...
Introduction    • Experimental Urology – internship 2009    • Polymer Chemistry – internship 2011         •     Targeted D...
PhD-project    • “Developing an artificial stoma for urinary diversion in      patients with extensive bladder disease”.  ...
Urinary Diversion (1/2)
Urinary Diversion (2/2)    • Urinary Diversion         •   Golden standard is Bricker ileal conduit         •   Bowel surg...
ProblemsIn 28-81% of the cases complications will occur, due to:• Intestinal surgery (1-5%)       •   Leakage/rupture of t...
Tissue engineering• Aims to restore or replace diseased, affected or non-  functional tissues.• In vitro and In vivo      ...
Artificial Urostomy (1/2)• Structural protein Collagen• (slow) Biodegradable Vypro® IIsynthetic polymer mesh• Porcine mode...
Artificial Urostomy (2/2)    • Problems          • Skin contraction          • Immunological reaction          • Tissue re...
Systematic Review    • “What is the current evidence for the efficacy of      tissue-engineered constructs for urinary div...
Search Strategy (in progress)                     Based on Leenaars et al (2012) 45: 24-31.
Inclusion/Exclusion        Used in Systematic Review
Why start a PhD with systematic Review?• To prevent unneccessary (animal) experimentation• To collect information about ma...
Experienced difficulties    • Commercial side of my research          • Unpublished information    • Negative results    •...
Conclusions   • Benefits and difficulties   • Do the benefits outweigh the difficulties?        • Time and effort        •...
Acknowledgements Department of Urology   SYRCLE Prof. Dr. Wout Feitz    Prof. Dr. Merel Ritskes-Hoitinga Dr. Egbert Ooster...
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SYRCLE_Sloff mini symposium sr animal studies 30082012

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Transcript of "SYRCLE_Sloff mini symposium sr animal studies 30082012"

  1. 1. Writing a Systematic Reviewa good start of a PhD-project Marije SloffPhD-student Experimental Urology Tissue Engineering
  2. 2. Introduction • Experimental Urology – internship 2009 • Polymer Chemistry – internship 2011 • Targeted Drug Delivery • Hyaluronan
  3. 3. PhD-project • “Developing an artificial stoma for urinary diversion in patients with extensive bladder disease”. • Patients • Bladder cancer •73.000 new patients/2012 in US •Muscle-invasive tumors • Congenital malformations •Spina bifida or bladder exstrophy •Neurogenic/dyfunctional bladder
  4. 4. Urinary Diversion (1/2)
  5. 5. Urinary Diversion (2/2) • Urinary Diversion • Golden standard is Bricker ileal conduit • Bowel surgery • Placing Urostomy
  6. 6. ProblemsIn 28-81% of the cases complications will occur, due to:• Intestinal surgery (1-5%) • Leakage/rupture of the anastomosis • Obstruction • Fistula formation • Infections• Unsuitability of tissue • Necrosis • Stenosis • Obstruction • Deformations
  7. 7. Tissue engineering• Aims to restore or replace diseased, affected or non- functional tissues.• In vitro and In vivo • Synthetic/biological materials • W/ or w/o cell technology• Scaffold • Temporal support • Promotes tissue regeneration • Replacement
  8. 8. Artificial Urostomy (1/2)• Structural protein Collagen• (slow) Biodegradable Vypro® IIsynthetic polymer mesh• Porcine model•Conclusion: A tubular construct can beused for urinary diversion (althoughimprovement is needed)
  9. 9. Artificial Urostomy (2/2) • Problems • Skin contraction • Immunological reaction • Tissue regeneration • Improvement • Material • Animal model • Porcine model • Predictive value?
  10. 10. Systematic Review • “What is the current evidence for the efficacy of tissue-engineered constructs for urinary diversion in animal models?” • PICO Patient: Urinary diversion in animal models Intervention: Tissue-engineered construct Comparison: Classical method using GI tissue Outcome: Survival/ regeneration/ inflammation/ integration/ functionality
  11. 11. Search Strategy (in progress) Based on Leenaars et al (2012) 45: 24-31.
  12. 12. Inclusion/Exclusion Used in Systematic Review
  13. 13. Why start a PhD with systematic Review?• To prevent unneccessary (animal) experimentation• To collect information about materials/methods/models• To generate a detailed overview of all relevant literature• To learn how to make a comprehensive search strategy
  14. 14. Experienced difficulties • Commercial side of my research • Unpublished information • Negative results • When to stop/continue • Slow proces
  15. 15. Conclusions • Benefits and difficulties • Do the benefits outweigh the difficulties? • Time and effort • Useful information • Extensive overview • Full-time focus at beginning of a project • Use for grant application
  16. 16. Acknowledgements Department of Urology SYRCLE Prof. Dr. Wout Feitz Prof. Dr. Merel Ritskes-Hoitinga Dr. Egbert Oosterwijk Dr. Rob de Vries Dr. Paul Geutjes
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