Gene Therapy                  for the            Treatment of CancerDAVID Y. DY, MD, MS, MBE, FPCS, FACSSurgical Oncologis...
Mig’s story
Migs’ Story2009 11 years old, boy, Filipino Painless blood in urine Cystoscopy: bleeding mass in bladder Dx: Rhabdoid ...
Migs’ Story Hospital in China   Locoregional and systemic chemotherapy          (Gemcitabine/Cisplatin)   Immunotherapy...
Mig’s story Progressive and metastatic      disease (R) flank pain Came back to Philippines Urologist: total excision ...
Mig’s storyGendicine: IT 9           IA  2           IV  1 + 6 Chemo (ICE)Outcome: No tumor seen on 2nd look surgery      ...
OUTLINE WHAT IS A GENE? HOW IS GENE MEDICINE MADE HOW DOES IT WORK? HOW IS IT USED? OTHER CASES
WHATISAGENE ?
ORGAN             ORGAN SYSTEMSHUMAN BODY                                     SEGMENT  CELLS                              ...
THECELL
CELLNUCLEUS
CHROMOSOMES23 pairs or 46chromosomes in thenucleus of each cellin our body
CHROMOSOME # 3•ONCOGENES•TUMORSUPPRESSORGENES
P53             TUMOR SUPPRESSOR GENEchromosome 17(17p13.1)
P53 tumor suppressor gene when a cells  DNA is  damaged, it  acts as an  "emergency  brake" to stop  the resulting  cell ...
P53 tumor suppressor gene It also acts as  an  executioner,  programming  damaged cells  to self-destruct  before their  ...
P53 tumor suppressor gene when it mutates, p53 can lose its suppressive powers or have the devastating effect of actually...
HOW ISGENE THERAPY MEDICINEMADE?
TWO COMPONENTS                 ADENOVIRUS p53 gene
Genetic Engineering             Adenovirus                                                  P53 Gene                      ...
HOW DOESGENE THERAPYWORK?
 The adenovirus  containing the  p53 tumor  suppressor gene  binds to the  receptor in the  cell membrane of  the cancer ...
 The  adenovirus is  then  packaged in a  vesicle The vesicle  proceeds to  the cell  nucleus
 The vesicle  breaks down,  releasing the  adenovirus  near the cell  nucleus The  adenovirus  injects its  gene, which ...
 The  adenovirus  injects its  gene, which  now includes  the p53  tumor  suppressor  gene, into the  cell nucleus
 The cancer  cell then  makes p53  protein
 The p53  protein  causes the  cancer cell to  self destruct  without  affecting  surrounding  normal cells
HOW ISGENE THERAPYADMINISTERED?
Direct injection to tumor
Injection into pleural and peritoneal cavities
Intravenous infusion
Intra arterial infusion
P53 Gene Therapy is            Safe• First used since year 2003• Over 16,000 patients already treated• No serious side eff...
OTHER PATIENTS
Liver Cancer Male, 60 y.o.•治疗前   before treatment   介入 + 基因治疗后   after interventional therapy+gene therapy
Advanced Lung CancerMale, 62 y.o.  Before treatment     after chemotherapy+gene therapy
Lung cancerMale, 55 y.o.
Cancer of the lower lipMale, 67 y.o..
GENE THERAPY
GENE THERAPY
Landline: 63-2-9618035   Mobile: +639175536300Email: surg.onc1@tullamarine.ph
Gene therapy - a Powerpoint Presentation
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Gene therapy - a Powerpoint Presentation

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A short introduction to what is gene therapy for cancer.

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Gene therapy - a Powerpoint Presentation

  1. 1. Gene Therapy for the Treatment of CancerDAVID Y. DY, MD, MS, MBE, FPCS, FACSSurgical OncologistAMY G. DY, MD, FPPS, FPSMO, FPSPOPediatric Oncologist
  2. 2. Mig’s story
  3. 3. Migs’ Story2009 11 years old, boy, Filipino Painless blood in urine Cystoscopy: bleeding mass in bladder Dx: Rhabdoid tumor of the urinary bladder Tx: Partial removal of urinary bladder
  4. 4. Migs’ Story Hospital in China  Locoregional and systemic chemotherapy (Gemcitabine/Cisplatin)  Immunotherapy  2 mos: pelvic recurrence  Cryosurgery  Brachytherapy
  5. 5. Mig’s story Progressive and metastatic disease (R) flank pain Came back to Philippines Urologist: total excision of bladder and rectum
  6. 6. Mig’s storyGendicine: IT 9 IA 2 IV 1 + 6 Chemo (ICE)Outcome: No tumor seen on 2nd look surgery PET CT : no active disease
  7. 7. OUTLINE WHAT IS A GENE? HOW IS GENE MEDICINE MADE HOW DOES IT WORK? HOW IS IT USED? OTHER CASES
  8. 8. WHATISAGENE ?
  9. 9. ORGAN ORGAN SYSTEMSHUMAN BODY SEGMENT CELLS OF COLON TISSUE
  10. 10. THECELL
  11. 11. CELLNUCLEUS
  12. 12. CHROMOSOMES23 pairs or 46chromosomes in thenucleus of each cellin our body
  13. 13. CHROMOSOME # 3•ONCOGENES•TUMORSUPPRESSORGENES
  14. 14. P53 TUMOR SUPPRESSOR GENEchromosome 17(17p13.1)
  15. 15. P53 tumor suppressor gene when a cells DNA is damaged, it acts as an "emergency brake" to stop the resulting cell division that can lead to cancer.
  16. 16. P53 tumor suppressor gene It also acts as an executioner, programming damaged cells to self-destruct before their altered DNA can be replicated.
  17. 17. P53 tumor suppressor gene when it mutates, p53 can lose its suppressive powers or have the devastating effect of actually promoting abnormal cell growth p53 is the most commonly mutated gene found in human tumors
  18. 18. HOW ISGENE THERAPY MEDICINEMADE?
  19. 19. TWO COMPONENTS ADENOVIRUS p53 gene
  20. 20. Genetic Engineering Adenovirus P53 Gene ns ert I bad gene Remove rAd-p53 RECOMBINANT HUMAN ADENOVIRUS p53 INJECTION MEDICINE
  21. 21. HOW DOESGENE THERAPYWORK?
  22. 22.  The adenovirus containing the p53 tumor suppressor gene binds to the receptor in the cell membrane of the cancer cell
  23. 23.  The adenovirus is then packaged in a vesicle The vesicle proceeds to the cell nucleus
  24. 24.  The vesicle breaks down, releasing the adenovirus near the cell nucleus The adenovirus injects its gene, which contain the p53
  25. 25.  The adenovirus injects its gene, which now includes the p53 tumor suppressor gene, into the cell nucleus
  26. 26.  The cancer cell then makes p53 protein
  27. 27.  The p53 protein causes the cancer cell to self destruct without affecting surrounding normal cells
  28. 28. HOW ISGENE THERAPYADMINISTERED?
  29. 29. Direct injection to tumor
  30. 30. Injection into pleural and peritoneal cavities
  31. 31. Intravenous infusion
  32. 32. Intra arterial infusion
  33. 33. P53 Gene Therapy is Safe• First used since year 2003• Over 16,000 patients already treated• No serious side effects• Common side effects are self-limited fever
  34. 34. OTHER PATIENTS
  35. 35. Liver Cancer Male, 60 y.o.•治疗前 before treatment 介入 + 基因治疗后 after interventional therapy+gene therapy
  36. 36. Advanced Lung CancerMale, 62 y.o. Before treatment after chemotherapy+gene therapy
  37. 37. Lung cancerMale, 55 y.o.
  38. 38. Cancer of the lower lipMale, 67 y.o..
  39. 39. GENE THERAPY
  40. 40. GENE THERAPY
  41. 41. Landline: 63-2-9618035 Mobile: +639175536300Email: surg.onc1@tullamarine.ph

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