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  • 1. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 PurePulse for Dummies - 1 Background of cell perforation using PEF Myriam Knopf Application Technologist PEF www.top-bv.nl www.toptechnologytalks.nl www.tophealthfacts.nl www.topfoodlab.nl www.topwiki.nl
  • 2. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 Background – freshly squeezed, tasty & healthy
  • 3. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 Unfortunnaly microbes are present in the juice …
  • 4. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 And if we do nothing, exponential growth occurs
  • 5. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 Even outbreaks due to pathogens may occur … Pathogeen Jaar Aantal zieken Aantal doden Oorzaak Land Salmonella Typhimurium 1944 18 1 Restaurant medewerker (zelf geen symptomen) Cleveland, Ohio, US Enterogene E. coli 1992 6 0 Slechte hygiëne verkopers India Salmonella Hartford, Gaminara, Rubislaw 1995 63, 7 opgenomen ziekenhuis 0 Amfibieën (padden) Orlando, Florida, US Shigella flexneri 1995 14 - geen verdere details Zuid-Afrika Salmonella enterica 1999 500 0 Sinaasappels besmet Adelaide, Australië Salmonella muenchen 1999 423 1 Mexicaans sinaasappelsap Tempe, Arizona, US Salmonella Anatum 1999 6 0 Straat verkoper, slechte hygiëne. Florida, US Salmonella Typhimurium 1999 405 0 Pathogeen ongeopende verpakkingen Australie Salmonella enteritidis 2000 74 0 Bron is niet bekend. Calfornia, US Salmonella Typhimurium en SaintPaul 2005 152 - HACCP slecht gehandhaaft Meerdere staten, US Salmonella Panama 2008 15 0 Besmet vruchtensap Nederland
  • 6. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 But, suppose we put a penlight on the juice … + + + + + + + + - - - - - - - - 1,5V
  • 7. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 … that provides an electric field distribution … + + + + + + + + - - - - - - - - 1,5V
  • 8. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 .. due to this E-field, the membrane perforates .. + + + + + + + + - - - - - - - - 1,5V
  • 9. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 .. and the microbes viability is gone immediately R.I.P. + + + + + + + + - - - - - - - -
  • 10. ir. Myriam Knopf (www.top-bv.nl) - PurePulse for Dummies - © 2013 The result? Healthy juice with sufficient shelf-life 21-60 days