A wide ranging scientificfield which includes themanipulation of livingorganisms that results innew products orprocesses by that cell.
Gregor Johann MendelDiscovered the LawsGoverning the GeneticInheritance of Traits byScientific Experimentation.Founded Modern Genetics.
*Creation of RECOMBINANTDNA*GENE CLONING*GENE TRANSFER
When DNA is extracted from an organism, all its genes are obtained In gene (DNA) cloning a particular gene is copied (cloned)
WHAT IS GENE CLONING ?• To "clone a gene" is to make many copies of it• Act of making many identical copies of gene• Gene can be an exact copy of a natural gene• Gene can be an altered version of a natural
Cell based DNA cloning Cell-free DNA cloning (PCR)
Blood sample DNA is extracted- here from blood DNA Restriction enzymes, e.g. EcoRI, HindIII, etc., cut the DNA into small pieces Different DNA pieces cut with the same enzyme can join, or recombine. RESTRICTION ENDONUCLEASE
BIOTECHNOLOGICALAPPLICATIONS IN MEDICINE ProteinPharmaceuticals Vaccines & Therapeutic Agents Diagnostics: Protein or DNA Based Gene Therapy Stem Cell Research Cancer Treatment
Insertion of a new “healthy” gene into the organism to provide needed (usually) proteins, hormones etc. Gene is carried into the host by a viral vector that has been disabled Can provide relief for many genetic diseases Problems: Jessie Gelsinger, xscid babies immune responses, and cancer