A wide ranging scientificfield which includes themanipulation of livingorganisms that results innew products orprocesses b...
Gregor Johann MendelDiscovered the LawsGoverning the GeneticInheritance of Traits byScientific Experimentation.Founded Mod...
*Creation of   RECOMBINANTDNA*GENE CLONING*GENE TRANSFER
 When   DNA is  extracted from  an organism, all  its genes are  obtained In gene (DNA)  cloning a  particular gene is  ...
WHAT IS GENE CLONING ?•    To "clone a gene" is to      make many copies of    it•    Act of making many      identical ...
Cell based DNA cloning   Cell-free DNA cloning (PCR)
Blood sample DNA is extracted-  here from blood                                   DNA Restriction  enzymes, e.g.  EcoRI,...
PCR technique                  ds DNAStep 1                   DenatureStep 2                   AnnealStep 3               ...
1st cycle     2nd cycle                 3rd cycle                 4th cycle                 5th cycle                 6th ...
* Flavr Savr Tomato*Golden Rice *BT Cotton * BT Brinjal
DELAYED RIPENING IN TOMATO
VIRUS RESISTANT TOMATO
NORMAL   GOLDENRICE     RICE
BIOTECHNOLOGICALAPPLICATIONS IN MEDICINE ProteinPharmaceuticals Vaccines & Therapeutic  Agents Diagnostics: Protein or ...
 Insertion of a new “healthy” gene into the  organism to provide needed (usually)  proteins, hormones etc. Gene is carri...
ORAL          VACCINEHUMULIN
1983 Transgenic Mice   1997 Cloned sheep
THANKSSS !!!
Biotechnology
Biotechnology
Biotechnology
Biotechnology
Biotechnology
Biotechnology
Biotechnology
Biotechnology
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Biotechnology

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Biotechnology

  1. 1. A wide ranging scientificfield which includes themanipulation of livingorganisms that results innew products orprocesses by that cell.
  2. 2. Gregor Johann MendelDiscovered the LawsGoverning the GeneticInheritance of Traits byScientific Experimentation.Founded Modern Genetics.
  3. 3. *Creation of RECOMBINANTDNA*GENE CLONING*GENE TRANSFER
  4. 4.  When DNA is extracted from an organism, all its genes are obtained In gene (DNA) cloning a particular gene is copied (cloned)
  5. 5. WHAT IS GENE CLONING ?• To "clone a gene" is to make many copies of it• Act of making many identical copies of gene• Gene can be an exact copy of a natural gene• Gene can be an altered version of a natural
  6. 6. Cell based DNA cloning Cell-free DNA cloning (PCR)
  7. 7. Blood sample DNA is extracted- here from blood DNA Restriction enzymes, e.g. EcoRI, HindIII, etc., cut the DNA into small pieces Different DNA pieces cut with the same enzyme can join, or recombine. RESTRICTION ENDONUCLEASE
  8. 8. PCR technique ds DNAStep 1 DenatureStep 2 AnnealStep 3 Extend
  9. 9. 1st cycle 2nd cycle 3rd cycle 4th cycle 5th cycle 6th cycle
  10. 10. * Flavr Savr Tomato*Golden Rice *BT Cotton * BT Brinjal
  11. 11. DELAYED RIPENING IN TOMATO
  12. 12. VIRUS RESISTANT TOMATO
  13. 13. NORMAL GOLDENRICE RICE
  14. 14. BIOTECHNOLOGICALAPPLICATIONS IN MEDICINE ProteinPharmaceuticals Vaccines & Therapeutic Agents Diagnostics: Protein or DNA Based Gene Therapy Stem Cell Research Cancer Treatment
  15. 15.  Insertion of a new “healthy” gene into the organism to provide needed (usually) proteins, hormones etc. Gene is carried into the host by a viral vector that has been disabled Can provide relief for many genetic diseases Problems: Jessie Gelsinger, xscid babies immune responses, and cancer
  16. 16. ORAL VACCINEHUMULIN
  17. 17. 1983 Transgenic Mice 1997 Cloned sheep
  18. 18. THANKSSS !!!
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