F LIMPOPO   UN         YO     I V ERSITFaculty of Science and Agriculture  Host-suitability and Host-sensitivity of Vernon...
15/05/12                                                Faculty of Agriculture and Science-Post-graduate Research Day     ...
15/05/12                                            Faculty of Agriculture and Science-Post-graduate Research Day         ...
15/05/12                                         Faculty of Agriculture and Science-Post-graduate Research Day            ...
15/05/12                                  OBJECTIVETo determine Host-suitability and Host-sensitivity of Vernonia galamens...
15/05/12                                                               Faculty of Agriculture and Science-Post-graduate Re...
15/05/12                                                Faculty of Agriculture and Science-Post-graduate Research Day     ...
15/05/12   Faculty of Agriculture and Science-Post-graduate Research Day                                                  ...
15/05/12                                                             Faculty of Agriculture and Science-Post-graduate Rese...
15/05/12                                                      Faculty of Agriculture and Science-Post-graduate Research Da...
15/05/12                  Faculty of Agriculture and Science-Post-graduate Research Day           ACKNOWLEDGEMENTS        ...
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Host suitability and host-sensitivity of vernonia galamensis accession 17, 18 and 19 to meloidogyne incognita race 2

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  • Banking Council 15/05/12
  • Host suitability and host-sensitivity of vernonia galamensis accession 17, 18 and 19 to meloidogyne incognita race 2

    1. 1. F LIMPOPO UN YO I V ERSITFaculty of Science and Agriculture Host-suitability and Host-sensitivity of Vernoniagalamensis accession 17, 18 and 19 to Meloidogyne incognita race 2 under Greenhouse conditions Matsobane Mathiba School of Agricultural and Environmental Sciences Department of Soil Science, Plant Production and Agricultural Engineering
    2. 2. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day INTRODUCTION Alternative vegetable oil: Vernonia oil valued for its vernolic fatty acid Synthetic epoxies of linoleic and alpha-linolenic acids are common substitutes of the carcinogenetic acryllic and phthalate products. Industrial epoxidation of common fatty acids increase greenhouse emissions Page 2
    3. 3. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day Vernonia galamensis in Limpopo Province V. galamensis is adaptable to the agronomic conditions in Limpopo Province, Republic of South Africa. Three accessions: 17, 18 and 19, have shown good adaptations in Limpopo Province and are therefore being considered as commercial lines. Root-knot nematodes: Meloidogyne incognita race 2 and M. javanica. Page 3
    4. 4. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day Three types of host-nematode interactions Susceptible host: Host allows nematodes to reproduce and the host suffers yield loss. Tolerant host: Host allows nematodes to reproduce, but the host does not suffer yield loss. Resistant host: Host does not allow nematodes to reproduce and there is no yield loss. Page 4
    5. 5. 15/05/12 OBJECTIVETo determine Host-suitability and Host-sensitivity of Vernonia galamensis accession17, 18 and 19 to Meloidogyne incognita race 2 under greenhouse conditions Page 5
    6. 6. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day MATERIALS AND METHOD Study area: Greenhouse Experimental design, treatment and procedure:  Two 5 x 3 Factorial experiments in RCBD with five replicates.  Treatments 0, 500, 1000, 1500 and 2000 nematode densities (Pi) were inoculated on V. galamensis accession 17, 18 and 19. Data collection  Nematode numbers (Pf)  Shoot mass  Calculated reproductive factor (Rf) = Pf/Pi Data analysis  Data: ANOVA and Means: LSD Page 6
    7. 7. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day RESULTS AND DISCUSSION Accession 17 Accession 18 Accession 19 Inoculation Root Soil Root Soil Root Soil (Pi) 500 232.5 2407.8 267.7 2075.4 226.4 834.0 1000 151.7 2069.7 128.0 1409.0 159.0 672.4 1500 81.1 1260.8 82.3 1403.4 73.1 409.2 2000 18.5 876.1 32.2 983.6 32.8 313.4 Accession 17 Accession 18 Accession 19 Inoculation Pf RF Pf RF Pf RF (Pi) 500 2640.2 5.2a 2343.0 4.7a 1065.2 1.97a 1000 2221.4 2.2b 1531.3 1.5b 831.4 0.83b 1500 1342.0 0.9c 1491.3 1.0bc 482.3 0.32bc 2000 894.6 0.5c 1015.9 0.51c 346.2 0.17c Page 7
    8. 8. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day Page 8
    9. 9. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day Accession 17 Accession 18 Accession 19 Shoot Shoot ShootInoculation (Pi) mass (g) Rel* mass (g) Rel* mass (g) Rel* Control 35.7a - 37.8a - 41.9a - 500 15.0b - 57.98 26.4b - 30.16 30.9b - 26.25 1000 14.7b - 58.82 29.1b - 23.06 27.6b - 34.13 1500 13.0b - 63.59 29.7b - 21.43 32.8b - 21.72 2000 16.3b - 54.34 27.2b - 28.04 31.5b - 24.82Relative effect = (treatment/control – 1) 100 Page 9
    10. 10. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day CONCLUSION V. galamensis accessions tested are susceptible to M. incognita race 2. Future research will include screening all available accessions with the aim of grafting the three commercially used accessions with those with less commercial value but resistant to nematodes. Page 10
    11. 11. 15/05/12 Faculty of Agriculture and Science-Post-graduate Research Day ACKNOWLEDGEMENTS Page 11

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