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TNAU PDB- Tamil Nadu Agricultural University Proteome Database-Black gram proteome
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TNAU PDB- Tamil Nadu Agricultural University Proteome Database-Black gram proteome

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In India, blackgram is the main edible crop ranking fourth among the pulses. There is a need to increase the performance of pulse crops, particularly in developing countries, where most grain legume …

In India, blackgram is the main edible crop ranking fourth among the pulses. There is a need to increase the performance of pulse crops, particularly in developing countries, where most grain legume production is for human consumption and demand is increasing due to population increase.Investigation was carried out on the possible effects of artificial ageing on dry seeds of blackgram. We have analyzed the quantitative and qualitative proteome changes in artificially aged dry seeds compared with that in control and the results were presented herein.Fresh seeds taken as control and 6 days artificially seeds as test.There were totally 59 spots detected both in fresh and accelerated aged seeds. Among these 16 spots differentially were expressed by artificial ageing, including 4 up regulated spots and 12 down regulated ones.

The reference 2D-PAGE gel shows the position of each identified protein in control. The entire list of protein can also be obtained by selecting the crop variety displayed inside the table. By selecting the spot in the gel image the entire information about that protein in both control as well as test can be obtained.All the reference maps are also displayed under 2D gel section for a quick reference.

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  • 1. Tamil Nadu Agricultural University Proteome DataBase(TNAU PDB)-Black Gram ProteomeVisit TNAU Genomics at www.tnaugenomics.com
  • 2. Introduction• TNAU PDB (TNAU Proteome DataBase) is a data repository ofvarious biotic and abiotic stress related proteins which areidentified from various organism using proteomics approach.Visit TNAU Genomics at www.tnaugenomics.com
  • 3. Need for Black Gram Database• The basic intention of this database is to provide the detailedinformation about proteome of black gram.• Results in improved understanding of adaptive and generalprotective mechanisms, conferring enhanced stress toleranceto plants.• Assists in building a comprehensive platform for future Blackgram proteomicsVisit TNAU Genomics at www.tnaugenomics.com
  • 4. Workflow Client tierApplication serverDatabaseVisit TNAU Genomics at www.tnaugenomics.com
  • 5. Generation of Data• Blackgram seeds were artificially aged at 40°C (8.98%moisture content) for 6 days, and the total protein wasextracted from embryos of the dry seeds.• Two-dimensional electrophoresis was performed and thedifferentially expressed proteins were identified by matrix-assisted laser desorption ionization-time of flight massspectrometry.Visit TNAU Genomics at www.tnaugenomics.com
  • 6. Schematic representation of proteomedata architectureVisit TNAU Genomics at www.tnaugenomics.com
  • 7. Black Gram Proteome DatabaseVisit TNAU Genomics at www.tnaugenomics.com
  • 8. ResultVisit TNAU Genomics at www.tnaugenomics.com
  • 9. Database features• All the reference maps are also displayed under 2D gelsection for a quick reference.• The experiment protocols are listed under the protocolsection.• Some of the major proteomics tools like includes Mascot,Compute pI/Mw tool in ExPASy and ExPASy Proteomics toolsare displayed under proteome tools section.Visit TNAU Genomics at www.tnaugenomics.com
  • 10. Future ProspectsVisit TNAU Genomics at www.tnaugenomics.com
  • 11. Reference• Murukarthick, J., N. Senthil, M. Raveendran, P. Prabhakaran, G.Sreedevi, M. Sumanth Kumar, V. G. Shobhana, S. Dhanya, R. Khushboo,S. Arumugasamy, R. Ravikesavan and S. V. K. Jagadish. 2011. BiogenBase – An Interactive Maize Database for Phenomics Platform.International Journal of Computer Application. 2: 56–61• Setsuko Komatsu, Keiichi Kojima, Kouji Suzuki, Kazuo Ozaki and KenichiHigo. 2004. Rice Proteome Database based on two-dimensionalpolyacrylamide gel electrophoresis: its status in 2003. Nucleic AcidsResearch, Vol. 32, Database issue.Visit TNAU Genomics at www.tnaugenomics.com
  • 12. Visit TNAU Genomics at www.tnaugenomics.comProject Guide : Dr.N.Senthil, ProfessorPresented by,S.SWATHI BTI-09-028