HUMAN ANTIBODIES FROM TRANSGENIC     ANIMALS                                  S. Kumarakurubaran,     CENTRE FOR PLANT MOL...
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     2
IntroductionMonoclonal antibodies has been extensively  used from its discoveryThese monoclonal antibodies are used in the...
CELL CULTURE Itis defined as the culturing up  of individual cells or by  disaggregated cell mass, either  totipotent or ...
THREE ROUTES FOR DESTINATION ANIMALCELL CULTURE TRANSGENIC ANIMALS IMMUNOLOGY OF MAbs           HUMAN ANTIBODIES FROM  ...
HISTORY Alexis  carrel(1912)-chick embryo tissue  extract increases cell proliferation in vitro Fibrin clot plasma used ...
Contd…. 1966 Alec Issacs discovered filtrates have  secretions called interferon Chinese hamster ovary cells were cultur...
CHARACTERISTICS OF ANIMALCELL Grow  only to a limited number of  generations Neurons cannot divide to grow Skin cells g...
SUBSTRATESPlastics-polystyrenes,                    PTTES, TPS, PVCGlass, methyl cellulosesMetallic surfaceAdhesives-d...
MEDIApH ,osmotic pressure, gas phasesNATURAL MEDIAPlasma clotsBiological fluidsSYNTHETIC MEDIASerum containingSerum f...
COMPOSITION Serum Proteins Carbohydrates Co factors Examples-MEM,CMRL media               HUMAN ANTIBODIES FROM      ...
CELL LINES Finite   cell lines-Epidermal  cells, Infinite cell lines- Cancerous  cells                HUMAN ANTIBODIES F...
MODEL ORGANISMMICE-short oestrous cycle, and gestation period-relatively short generation time-several litters per pregnan...
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     14
Transgenic animals Method of introducing transgeneTransfection Transient-transgene gradually lost   from daughter cells...
Transfection methods Calcium phosphate precipitation DEAE-dextran mediated transfection Lipofection Retroviral vector ...
HUMAN ANTIBODIES FROM   Gardener et al., 2003 TRANSGENIC ANIMALS                             17
Gardener et al., 2003HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS                       18
VECTORS Simian virus 40 Bovine papilloma virus with transforming  region of pBR322 Polyoma virus Vaccinia virus P ele...
Gene construct Promoter/enhancer element   RSV, SV40, β-casein Initiation codon, Gene of interest Reporter gene scorab...
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     21
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     22
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     23
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     24
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     25
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     26
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     27
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     28
Milstein and Kohler 1975                  HUMAN ANTIBODIES FROM                   TRANSGENIC ANIMALS     29
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS     30
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS                          31                        Nil Lonberg (2005)
HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS                        Nil Lonberg (2005   32
Nil Lonberg (2005)HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS                          33
MONOCLONAL ANTIBODYDerived from single cell line have identical structure binds same epitope,Specific antigen is repeatedl...
ApplicationsHighly sensitive (magic bullets) epitopes  helps in in vitroImmuno diagnostics (detection and  quantification)...
Negative effects Antibodies recognized as murine  antibodies and mounts immune response  and eliminates from the body. C...
Chimerised & human antibodies Variable region is obtained from non  human rat antibody Constant region is obtained from ...
Contd.. Humanised to make more compatible Utilizes recombinant DNA technology Epitopes are substituted with human gene ...
Human monoclonal antibodies  from SCID mouse by injecting human B  and T cells and grafting mouse by  transplanting splee...
Hybridoma technology   First discovered by Milstein and Kohler 1975   Specific antigen is given repeatedly to the host ...
HUMAN ANTIBODIES FROM   William Fodor                                 41 TRANSGENIC ANIMALS                             2004
CHARACTERISTICSPOLYCLONAL ANTIBODY               MONOCLONAL ANTIBODY   Contains many                    Contains single ...
CONCLUSION  HUMAN ANTIBODIES FROM   TRANSGENIC ANIMALS     43
HUMAN ANTIBODIES FROM   44 TRANSGENIC ANIMALS
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Human antibodies from transgenic animals

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Human antibodies from transgenic animals

  1. 1. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS S. Kumarakurubaran, CENTRE FOR PLANT MOLECULAR BIOLOGY
  2. 2. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 2
  3. 3. IntroductionMonoclonal antibodies has been extensively used from its discoveryThese monoclonal antibodies are used in the field of medicine for therapeutic applicationsThe research in the field of immunology has flourished to produce human antibodies from transgenic animals HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 3
  4. 4. CELL CULTURE Itis defined as the culturing up of individual cells or by disaggregated cell mass, either totipotent or pleuripotent, in a suitable culture medium HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 4
  5. 5. THREE ROUTES FOR DESTINATION ANIMALCELL CULTURE TRANSGENIC ANIMALS IMMUNOLOGY OF MAbs HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 5
  6. 6. HISTORY Alexis carrel(1912)-chick embryo tissue extract increases cell proliferation in vitro Fibrin clot plasma used as solid support Ross harrison(1907)cultured embryonic nerve cell of frog Using Hanging drop method Carcinoma cell line HE LA, myeloma cells identified HUMAN ANTIBODIES FROM TRANSGENIC 6 ANIMALS
  7. 7. Contd…. 1966 Alec Issacs discovered filtrates have secretions called interferon Chinese hamster ovary cells were cultured High density culturing methods were found Stem cell technology was discovered 1996 Dolly was produce by egg enucleation HUMAN ANTIBODIES FROM TRANSGENIC 7 ANIMALS
  8. 8. CHARACTERISTICS OF ANIMALCELL Grow only to a limited number of generations Neurons cannot divide to grow Skin cells grow only for a few generation Process of contact inhibition HUMAN ANTIBODIES FROM TRANSGENIC 8 ANIMALS
  9. 9. SUBSTRATESPlastics-polystyrenes, PTTES, TPS, PVCGlass, methyl cellulosesMetallic surfaceAdhesives-d lysine, gelatin,Feeder layer for glial cells HUMAN ANTIBODIES FROM TRANSGENIC 9 ANIMALS
  10. 10. MEDIApH ,osmotic pressure, gas phasesNATURAL MEDIAPlasma clotsBiological fluidsSYNTHETIC MEDIASerum containingSerum free HUMAN ANTIBODIES FROM TRANSGENIC 10 ANIMALS
  11. 11. COMPOSITION Serum Proteins Carbohydrates Co factors Examples-MEM,CMRL media HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 11
  12. 12. CELL LINES Finite cell lines-Epidermal cells, Infinite cell lines- Cancerous cells HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 12
  13. 13. MODEL ORGANISMMICE-short oestrous cycle, and gestation period-relatively short generation time-several litters per pregnancy-Convenient in vitro fertilization-successful in vitro embryo culture-production and maintenance of ES lines HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 13
  14. 14. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 14
  15. 15. Transgenic animals Method of introducing transgeneTransfection Transient-transgene gradually lost from daughter cells Stable-retained and expressed in all the cells derived from transfected cells HUMAN ANTIBODIES FROM 15 TRANSGENIC ANIMALS
  16. 16. Transfection methods Calcium phosphate precipitation DEAE-dextran mediated transfection Lipofection Retroviral vector infection Microinjection Embryonic stem cell transfer Mark.M.Davis (2004) HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 16
  17. 17. HUMAN ANTIBODIES FROM Gardener et al., 2003 TRANSGENIC ANIMALS 17
  18. 18. Gardener et al., 2003HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 18
  19. 19. VECTORS Simian virus 40 Bovine papilloma virus with transforming region of pBR322 Polyoma virus Vaccinia virus P element vectors Baculovirus vectors HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 19
  20. 20. Gene construct Promoter/enhancer element RSV, SV40, β-casein Initiation codon, Gene of interest Reporter gene scorable marker β- galactosidase, lux selectable marker-thymidine kinase Termination signal, Poly A tail HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 20
  21. 21. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 21
  22. 22. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 22
  23. 23. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 23
  24. 24. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 24
  25. 25. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 25
  26. 26. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 26
  27. 27. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 27
  28. 28. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 28
  29. 29. Milstein and Kohler 1975 HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 29
  30. 30. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 30
  31. 31. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 31 Nil Lonberg (2005)
  32. 32. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS Nil Lonberg (2005 32
  33. 33. Nil Lonberg (2005)HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 33
  34. 34. MONOCLONAL ANTIBODYDerived from single cell line have identical structure binds same epitope,Specific antigen is repeatedly injected and and spleen cells are extracted to obtain MAbs HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 34
  35. 35. ApplicationsHighly sensitive (magic bullets) epitopes helps in in vitroImmuno diagnostics (detection and quantification)Used in treatment of cancer, delivers drugs specifically to target tumor cells. HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 35
  36. 36. Negative effects Antibodies recognized as murine antibodies and mounts immune response and eliminates from the body. Causes serious allergenicity to the level of lethality. Repeated use of mouse antibodies induces host to produce antibodies that neutralizes mouse antibody called HAMA HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 36
  37. 37. Chimerised & human antibodies Variable region is obtained from non human rat antibody Constant region is obtained from human sequence Chimerics exhibit lower immunogenicity to the host Still the chimeric sequence requires changes this is done by r-DNA by engineering specific amino acid sequence HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 37
  38. 38. Contd.. Humanised to make more compatible Utilizes recombinant DNA technology Epitopes are substituted with human gene sequences Constant regions are derived from human HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 38
  39. 39. Human monoclonal antibodies from SCID mouse by injecting human B and T cells and grafting mouse by transplanting spleens and lymph nodes The transplanted mouse is immunized with target antigens to produce human antibodies Phage derived combinatorial antibody library, phage display technique-mimics in vitro affinity maturation HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 39
  40. 40. Hybridoma technology First discovered by Milstein and Kohler 1975 Specific antigen is given repeatedly to the host The spleen producing B cells are isolated B cells are fused with myeloma cells that are immortal and this is called hybridoma cells This produces specific antibody indefinitely precisely can be isolated easily HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 40
  41. 41. HUMAN ANTIBODIES FROM William Fodor 41 TRANSGENIC ANIMALS 2004
  42. 42. CHARACTERISTICSPOLYCLONAL ANTIBODY MONOCLONAL ANTIBODY Contains many  Contains single specific antigenic determinants determinant Produces various  Single class of antibody classes is produced Reproducibility and  Highly reproducible standardization is difficult HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 42
  43. 43. CONCLUSION HUMAN ANTIBODIES FROM TRANSGENIC ANIMALS 43
  44. 44. HUMAN ANTIBODIES FROM 44 TRANSGENIC ANIMALS
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