Davis plaque method.pptx recombinant DNA technology
Country Workplan_Cowpea-Nigeria_TL III Annual Meet
1. Tropical Legumes III (Cowpea-
Nigeria)
2016 Country Workplan
Objective 3: To enhance cowpea
productivity and production
Tropical Legumes III
2. 3.1 Trait Discovery Pipeline
3.1.1 Multiplication and phenotyping of
MARS lines for Striga resistance, Drought
tolerance and seed size.
• Multiplication of 150 MARS lines.
• Phenotyping of the MARS lines for Striga
resistance, drought tolerance and seed size
3. 3.1 Trait Discovery Pipeline Contn’d
3.1.2 Collection and evaluation of
phenotyping data using modern tools
and IBP.
• Training of scientists and technicians on
Breeding Management System of IBP.
• Creation of a local database for phenotyping,
genotyping and pedigree in IAR.
4. 3.2 Breeding Pipeline
3.2.1: Evaluation of MARS lines for low P
tolerance
• Ten low-P tolerant breeding lines selected
3.2.2: Initiate MABC scheme
• For each trait (Aphids, Striga and Bacterial
blight) Identify preferred variety to improve
• Hybridize and generate BC1.
5. 3.3 Testing for Release
3.3.1 Evaluation of promising short and
medium duration lines.
• Conduct advanced yield trials (Short and Medium
duration) in four (4) locations (Minjibir, Samaru, Bakura
and Dutsen-Ma), with at least 20 entries each.
• Conduct multi-locational trials (short and medium) in
at least 8 locations (Minjibir, Samaru, Bakura, Dutsen-
Ma, Bauchi, Makurdi, Ibadan and Abeokuta).
• Produce at least 50 kg seed of each of 5 selected
promising breeding lines
6. 3.3 Testing for Release Contn’d
3.3.2: On-farm evaluation of new
varieties (FPVS)
• Evaluate three new varieties in on-farm trials in at
least 100 locations (in Kano, Jigawa, Katsina and
Sokoto States) with at least 2000 participants
(minimum of 30% women)
3.3.3: Nutrition profiling of candidate
release varieties
• Determine nutrition profile of 5 most promising
candidate varieties prior to release
7. 3.4: Best Bet Varieties
3.4.1 On-farm demonstration of new technologies
• Establish at least fifteen (15) demonstration plots of two newly-
released cowpea varieties (SAMPEA-16 and 17) each in Kano,
Jigawa, Katsina and Sokoto States (total = 60).
3.4.2 Breeder Seed Production
• Establish three (3) hectares of Breeder seed farm of 4 newly-
released varieties (IT99K-573-1-1, IT99K-573-2-1, IT07K-318-33 and
IT07K-292-10 )to obtain a minimum of 2 tons of breeder seed.
3.4.3 DNA fingerprinting of released varieties
• Fingerprint 17 released varieties