buchanania lanzan hepatocarcinoma presentation


Published on

Published in: Education, Health & Medicine
1 Like
  • Be the first to comment

No Downloads
Total views
On SlideShare
From Embeds
Number of Embeds
Embeds 0
No embeds

No notes for slide
  • LPO – LIPID PEROXIDASESOD – superoxide dismutase
  • buchanania lanzan hepatocarcinoma presentation

    1. 1. Abstract Reference Number : OPP 030 Effect of total extract of Buchanania lanzan leaves against Hepatocellular Carcinoma(HCC) in Diethyl nitrosamine induced tumor model Sumithra M*, Anbu J, Srota Dawn, Ashwini Anjana, Nazeer Ahmed, Ravichandiran V. Department of Pharmacology, Vels University Pallavaram, Chennai-600117 Presenting Author Srota Dawn M . Pharm (Pharmacology)
    2. 2. Introduction: HCC is the most common type of liver malignancy and 4th most common cause of cancer mortality. Worldwide incidence >600,000 cases per year Liver cancer is the most rapidly increasing cancer in the U.S., Asia and Africa. 19,160 new cases and 16,780 deaths in 2007 More common in men than women (4:1) For resection, rate of recurrence can be as high as 50% at 2 years Only 12% are eligible for resection or for transplant 80%-90% of HCC cases occur in cirrhotic livers
    3. 3. Factors causing HCC : Environmental factors Hepatitis viral infection Food additives Excessive alcohol consumption and smoking Alfatoxins Exposure with chemical carcinogens Air and water pollutants
    4. 4. Management of HCC: Liver transplantation Resection Tumour ablation Radiofrequency thermal ablation Alcohol injection Chemoembolization Targeted molecular therapy Chemotherapy Regional Systemic
    5. 5. Bone marrow depression Lymphocytopenia, Stomatitis Gastro intestinal tract disorders like Diarrhoea, Nausea, Vomiting Skin problems like Alopecia, Dermatitis Oligozoospermia, Impotency in males Abortion and Fetal damage in females Generation of Secondary cancers specially leukemia, lymphoma
    6. 6. Aim : Here the anticancer potential of ethanolic extract of Buchanania lanzan (Anacardiaceae family) leaves was evaluated against Diethyl nitrosamine (DEN) induced hepatocarcinoma in male wistar rats. The aim of this study is to reduce the toxicity and adverse effect of cytotoxic drugs by the use of drugs from phytochemical sources.
    7. 7. About the Plant: Buchanania lanzan (Anacardiaceae family) is an evergreen deciduous tree, growing 50 ft tall. popularly known as “Chiranji” in Hindi Uses: Buchanania lanzan has folklore evidence for its effective role in curing asthma, cough, skin diseases, antioxidants and antitumor effect. All parts of the plant are used for the treatment of various diseases. The oils extracted from the seed are used for reduction of granular swelling of neck The kernel is used to treat skin diseases like itching and as prickly heat also The gum obtained from the bark of Buchanania lanzan used for treating diarrhoea and intercostals pains. Leaves of Buchanania lanzan tree are used for promoting wound healing
    8. 8. Work plan and procedure Collection and authentication of plant material Extraction procedure (soxhlet extractor, 70% ethanol, temperature 3540ºC for 72 hrs.); preliminary phytochemical studies Selection of animals(30 adult male wistar rats, 100-150 g); acclimatized for 2 weeks, 12 hrs. day/night cycle, pallet food, water ad libitum ; experimental procedure and protocol used in this study was reviewed and approved by Institutional Animal Ethics Committee (IAEC) The doses are fixed after performing the acute toxicity studies according to the OECD guideline 423 Continued….
    9. 9. Grouping of the selected animals Experimental animals are randomly divided into 5 groups (n=6) GROUP - І (control) GROUP – ІІ (DEN alone) GROUP - ІІІ (DEN+TEST 1 ; 200 mg/kg) GROUP – ІV (DEN+TEST 2 ; 400mg/kg) GROUP - V (DEN+ 5 Flurouracil) DEN (in DMSO) is given intraperitonially at a dose of 50 mg/kg once in a week for a period of three weeks to GROUP – ІІ, GROUP – ІІІ, GROUP - ІV and GROUP - V ; The test drug is given for 15 days; Continued.........
    10. 10. After 21 days of observation the overnight fasted animals were sacrificed by chemically induced euthanasia using chloroform. Blood samples were collected by cardiac puncture serum was obtained from the blood by centrifugation at 4,000 rpm for 20 minutes. The supernatant is used for estimation of Serum enzymes like SGPT, SGOT, ALP, total protein and bilirubin values were evaluated. determination of antioxidant enzymes, liver was dissected Using ice cold saline and homogenate was prepared (0.1M Tris–HCL buffer, pH 7.4) , centrifuged and the supernatant was used for the estimation of Tissue LPO , SOD and CAT level were estimated. All data obtained from the experiment were represented as mean ± SEM and analyzed by One way ANOVA and for multiple comparisons of groups with post hoc Bonferroni’s test was used.
    11. 11. Result and graphs: Table no. 1. Data showing preliminary Phytochemical screening of the ethanolic extract of Buchanania lanzan leaves extract. Sl. No. Phytochemical test Ethanolic extract of Buchanania lanzan leaves extract 1. Test for Alkaloids +ve 2. Test for Steroids -ve 3. Test for Tannins -ve 4. Test for Saponin -ve 5. Test for Gallic acid +ve 6. Test for Flavonoids +ve 7. Test for Carbohydrates -ve 8. Test for Gum and Mucilage’s -ve 9. Test for Glycoside +ve
    12. 12. Table no. 2. Effect of ethanolic extract of Buchanania lanzan leaves on Biochemical parameters in DEN induced Hepatocarcinoma in rats. Bilirubin (mg/dL) Groups (n=6) SGOT (IUL-1) SGPT (IUL-1) ALP (IUL-1) TP (mg/100g) Normal control 51.47±0.33 98.25±0.27 220.25±0.25 767.90±0.23 2. DEN alone 103.13±0.45 255.51±0.15 295.33±0.62 832.16±0.52 1.517±0.093 3. DEN+200mg/kg extract(T1) 91.21±0.23 242.98±0.34 288.12±0.33 818.32±0.43 0.510±0.06 4. DEN+400mg extract(T2) 84.45±0.65 180.43±0.33 252.65±0.22 792.21±0.21 0.536±0.12 5. DEN+ 5FU 81.11±0.54 167.55±0.56 247.55±0.93 788.34±0.99 0.510±0.05 Sl. no. 1. 0.460±0.022 All values are presented as mean ± SEM. Group II was compared with group I and all values were significant (p< 0.001); group III, group IV and group V were compared with group II and all values were significant. (p< 0.001). P values were calculated by one way ANOVA analysis with post hoc Bonferroni’s test. (N=6)
    13. 13. Graphical representation SGOT & SGPT level (IU/L) 300 Effect of Buchanania lanzan on SGOT and SGPT level in DEN induced Hepatocarcinoma in rats 250 200 150 255.51 242.98 100 180.43 50 98.25 103.13 91.21 84.45 167.55 81.11 51.47 0 Normal control DEN alone DEN+200 mg/kg extract(T1) DEN+400 mg extract(T2) DEN+ 5FU Groups SGOT (IUL-1) SGPT (IUL-1) * Reitman et.al 1957
    14. 14. Effect of Buchanania Lanzan on Biochemical parameters in DEN induced Hepatocarcinoma in rats. 1000 900 ALP and TP level 800 700 600 500 400 832.16 767.9 818.32 792.21 788.34 300 200 100 220.25 295.33 288.12 252.62 247.55 0 Normal control DEN alone DEN+200 mg/kg extract(T1) Groups DEN+400 mg/kg extract(T2) DEN+ 5FU ALP (IUL-1) TP (mg/100g)
    15. 15. 1.8 Effect of Buchanania lanzan on bilirubin level in DEN induced Hepatocarcinoma in rats. 1.6 Bilirubin level (mg/dl) 1.4 1.2 1 0.8 1.517 0.6 0.4 0.2 0.51 0.46 0.536 0.51 DEN+200mg/kg extract(T1) DEN+400mg/kg extract(T2) DEN+ 5FU 0 Normal control DEN alone Groups
    16. 16. Table no. 3. Effect of Buchanania Lanzan on Antioxidant level in DEN induced Hepatocarcinoma in rats. Sl. no. Groups (n=6) SOD CAT LPO 1. Normal control 8.89±0.12 63.21±1.45 0.095±.003 2. DEN alone 4.44±0.10 41.85±1.33 0.219±0.004 3. DEN+200mg extract(T1) 5.11±0.21** 48.55±1.09** 0.115±0.013 4. DEN+400mg extract(T2) 7.65±0.33 56.09±0.99 0.178±0.02** 5. DEN+ 5FU 7.99±0.55 57.99±1.93 0.099±0.003 All values are presented as mean ± SEM. Group II was compared with group I and all values were significant (p<0.001) Group III (p<0.01) vs. Group II Group IV (p<0.01) vs. Group II Group V (p<0.01) vs. Group II, N=6 SOD = µ moles of MDA/min/mg protein CAT = µ moles of H2O2 consumed/min/mg protein LPO = µ moles of MDA/min/mg protein
    17. 17. Effect of Buchanania Lanzan on SOD and CAT level in DEN induced Hepatocarcinoma in rats. 70 60 SOD and CAT level 50 40 SOD 63.21 30 57.99 56.09 48.55 41.85 20 10 8.89 7.65 4.44 7.99 5.11 0 Normal control DEN alone DEN+200mg/kg extract(T1) Groups DEN+400mg/kg extract(T2) DEN+ 5FU CAT
    18. 18. Effect of Buchanania Lanzan on LPO level in DEN induced Hepatocarcinoma in rats. 0.25 LPO level 0.2 0.15 0.219 0.1 0.05 0.178 0.115 0.095 0.099 0 Normal control DEN alone DEN+200mg/kg extract(T1) Groups DEN+400mg/kg extract(T2) DEN+ 5FU
    19. 19. Discussion: Elevation of the plasma levels of cytoplasmic and mitochondrial enzymes are sensitive indicators of liver damage. Elevation of serum SGPT, SGOT, ALP, TP, LPO and bilirubin is known effect of DEN toxicity which specially affects the liver and activities of ALP, SGPT, SGOT, and LPO are most commonly used biochemical markers of liver damage. Lipid peroxidation plays an important role in carcinogenesis There is a significant dose dependent reduction in SGOT , SGPT, ALP, TP level and SOD, CAT levels are increased in dose dependent manner in the B. lanzan treated groups. The low dose of extract shows a better effect in reduction of bilirubin and LPO level than the high dose.
    20. 20. Conclusion: The preliminary phytochemical studies reveal the presence of flavonoids, Alkaloids, Gallic acid and Glycoside in total extract of Buchanania lanzan. After studying many journals we can say that Flavonoid and alkaloids has a chemo preventive role in cancer. Therefore the possible mechanism of anticancer of Buchanania lanzan may be due to flavonoid or alkaloid content and further study has to be carried out to study the exact mechanism behind it and isolation of the active constituents present in the extract.
    21. 21. Acknowledgments The authors are thankful to Vels University, Pallavaram, Chennai – 600117, India, for providing the necessary laboratory facilities for this study. I want to thank my teacher Sumithra M. for giving me the opportunity to present this paper and I also want to thank the authority of NATIONAL CONFERENCE ON HERBAL MEDICINE (NCHM’13) for encouraging the students and research scholars and for giving a platform to present their views.