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Microbiology training module--finger dab& higeine
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Microbiology training module--finger dab& higeine

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Pharmaceutical Microbiology- Hygiene And Finger Dab Procedures

Pharmaceutical Microbiology- Hygiene And Finger Dab Procedures

Published in Health & Medicine
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  • 1. Quality Control-MicrobiologyTraining on hygiene & finger dab test Sreenath.S Site Microbiologist 1
  • 2. Over view of finger dab & hygiene• Cleanliness of the personnel• Maintenance of hygiene• Proper hand sanitization• Disinfectant cleaning• Hand Swabbing• Use of right sanitizer• Growth of organisms• Educating personnels 2
  • 3. Why Is Hand Hygiene Important?Hands are the most common mode of pathogen transmissionHand washing is an extremely important step Hand washing significantly removes the bacterial flora on the skin Hand washing protects you and your patient from the bacterial flora (from each other) from becoming skin residents 3
  • 4. Hand Hygiene Definitions:Hand washing − Washing hands with plain soap and water• Antiseptic hand wash − Washing hands with water and soap or other detergents containing an antiseptic agent• Alcohol-based hand rub − Rubbing hands with an alcohol- containing preparation (used after hand washing) 4
  • 5. When to Wash:• Visibly dirty• After touching contaminated objects with bare hands• Before glove placement and after glove removal Hand washing before and after each operation is the single most important hygiene measure for reducing or preventing the spread of contamination 5
  • 6. Rings and Jewelry • Hand jewelry should not be worn as it may tear gloves and harbor bacteria − Studies have demonstrated that skin underneath rings is more heavily colonized than comparable areas of skin on fingers without rings − the more rings worn, the greater concentration of organisms 6
  • 7. Fingernails• Can affect the integrity of gloves• Can also harbor bacteria• Keep fingernails SHORT! − Avoid artificial nails 7
  • 8. Wash hands (or use and alcoholbased rub) before glove placementand after glove removal. 8
  • 9. Hand Hygiene: Frequently Missed Areas 9
  • 10. Cleanliness of the personnel’s• Always clean your hands before entering into manufacturing area and where ever it is to be followed.• Disinfectant the gloves during operation. • Personnel Hygiene • Illness − Staff with illness or open lesions should not handle starting materials, intermediates or finished products • Adverse conditions − operators trained to recognize risks − willingness to report illness to the area supervisor 10
  • 11. Personnel HygieneHealth examinations – On recruitment for direct operators , repeated on regular basis• Training - check – induction training for new operators includes basic personal hygiene training – written procedures - to wash hands before entering a manufacturing area – signs in changing rooms to reinforce hand washing 11
  • 12. Personnel Hygiene• Contact between product and operator avoid direct contact if direct handling unavoidable, gloves should be worn check glove disinfection (for sterile production) and disposal 12
  • 13. Personnel Hygiene• Clothing and changing facilities − check changing rooms (hand washing, towels or hot air hand dryers) − check if used clothing stored in separate closed containers while awaiting cleaning − laundering of clean area clothing must be to SOP and in appropriate facility − check for procedure for sterilizing and storing clothing for use in sterile area 13
  • 14. FINGER DAB TEST• Frequency:• Once in six months• Sampling plan: (Random sampling)• Persons working in the manufacturing area are selected randomly from each section on the day of the test.• Limits:• For Bacteria: Standard limit: NMT 100 cfu/Personnel• Alert limit: NMT 50 cfu/ Personnel.• Action limit: NMT 75 cfu/ Personnel.• Fungi: Should be absent• Pathogens: Should be absent 14
  • 15. FINGER DAB TEST DEMO SAMPLE UNDER MICROBIAL SWAB TAKEN BEFORE HAND WASH TESTING AFTER INCUBATION COLONIES ARE IDENTIFIED 15
  • 16. FINGER DAB TEST DEMO SAMPLE TAKEN AFTER HAND SANITIZATION SAMPLE UNDER MICROBIAL TESTING AFTER INCUBATION NO COLONIES ARE IDENTIFIED 16
  • 17. Taking off the first glove:1. 2. 3. 17
  • 18. 4. 5. 18
  • 19. Taking off the second glove:1. 2. 19
  • 20. 4.3. 5. 20
  • 21. Disposal: 21
  • 22. THANK You……… Any Query ???????? 22