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SHUMEZ H.
HEINRICH AUSPITZ
Akantha - thorn
Lysis - loosening
Definition
 Loss of coherence between epidermal cells due to
the breakdown of their intercellular bridges.
 The cells re...
Basic Structures
 Desmosomal Cadherins are cell adhesion molecules
which bind one keratinocyte to another.
 They are of ...
 Desmogleins are of 3 types:
Dg1 - pemphigus foliaceous – upper layers of epidermis
Dg2 - all desmosome possessing tissue...
Pathogenesis
 Desmosomal Cadherins are the primary
pathological targets in acantholysis.
 Acantholysis is initially char...
 The acantholytic cells remain metabolically active
for some time and retain their capacity for DNA
synthesis.
 Another ...
Triggers
 Autoimmunity
 Drugs
 Infections
 Burns
 Genetics
 Heat, UV rays
 Ionizing radiation
 Contact dermatitis
...
Classification
Classified as Primary or Secondary
 Primary Acantholysis : In these diseases,
acantholysis is the primary ...
Causes of primary acantholysis
 Pemphigus vulgaris and its variants
 Pemphigus foliaceous and its variants
 Hailey - Ha...
Causes of secondary acantholysis
 Herpes simplex virus infections
 Herpes zoster
 Condyloma acuminatum
 BT leprosy
 E...
 Galli - Galli disease
 Solar keratosis
 Acantholytic acanthoma
 Adenoid squamous cell carcinoma
 Basal cell carcinom...
Acantholysis in Pemphigus
 The conventional concept is that PV IgG binds to
Dsgs resulting in stearic hindrance, interfer...
 Subsequently, tonofilaments collapse and
keratinocytes shrink with sloughing of
desmosomes, which elicits an autoimmune
...
Acantholysis in Hailey - Hailey disease
 Acantholysis results from a genetically determined
defect, resulting in desmosom...
Biochemical Acantholysis
 The term describes a special type of primary
acantholysis brought about without antibody
mediat...
Sites of acantholysis
 The site of acantholysis in a given condition helps
in the diagnosis, especially in blistering dis...
Clinical signs associated with
acantholysis
Nikolsky’s sign
 The sign is elicited by applying tangential
pressure with a ...
 (a) Eliciting Nikolsky's sign on perilesional skin. Note the
tangential pressure, (b) Eliciting Nikolsky's sign, peeling...
 The underlying pathophysiology of this sign is
that acantholysis occurs in affected areas as well
as in areas with intac...
Bulla spread sign (Asboe-Hansen sign)
 It is the enlargement of an intact blister by the
application of mechanical pressu...
Eliciting Bulla spread sign. Note the vertical pressure.
Tzanck Smear
 Acantholytic cells are easily demonstrated using a
side-lab cytologic smear.
 The base of an unroofed blis...
 It is a rounded keratinocyte with
 hypertrophic or dysmorphic nucleus,
 hazy or absent nucleoli,
 increased nuclear t...
Tzanck smear of pemphigus vulgaris showing abundant
acantholytic cells. (Giemsa stain, 10× )
High power view of Tzanck cells in pemphigus vulgaris. Note the
perinuclear halo visible in the marked cell. (Giemsa stain...
THANK YOU
Acantholysis
Acantholysis
Acantholysis
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Acantholysis

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Transcript of "Acantholysis"

  1. 1. SHUMEZ H.
  2. 2. HEINRICH AUSPITZ Akantha - thorn Lysis - loosening
  3. 3. Definition  Loss of coherence between epidermal cells due to the breakdown of their intercellular bridges.  The cells remain intact but are no longer attached to each other.  They tend to acquire the smallest possible surface area and become rounded up, resulting in intradermal clefts, vesicles and bullae.
  4. 4. Basic Structures  Desmosomal Cadherins are cell adhesion molecules which bind one keratinocyte to another.  They are of 2 types :  Transmembrane compounds - Desmoglein & Desmocollin  Cytoplasmic compounds - Desmoplakin & Plakoglobin  Cadherins are calcium ion dependent cell adhesion molecules.
  5. 5.  Desmogleins are of 3 types: Dg1 - pemphigus foliaceous – upper layers of epidermis Dg2 - all desmosome possessing tissues Dg3 – pemphigus vulgaris – basal & parabasal layers  Desmocollins are of 2 types: Dc1 & Dc2 - SCPD ( SubCorneal Pustular Dermatosis ) type of IgA pemphigus
  6. 6. Pathogenesis  Desmosomal Cadherins are the primary pathological targets in acantholysis.  Acantholysis is initially characterised by separation of the inter desmosomal regions followed by splitting and disappearance of desmosomes, forming intercellular gaps.  These intercellular gaps result in fluid influx from the dermis leading to cavity formation, which maybe suprabasal, mid - epidermal, subcorneal in location.
  7. 7.  The acantholytic cells remain metabolically active for some time and retain their capacity for DNA synthesis.  Another important molecule in the pathogenesis of acantholysis is syndecan - 1, a heparan sulfate proteoglycan on the keratinocyte membrane, which functions in intercellular adhesion.  Absent or markedly decreased syndecan - 1 is expressed by acantholytic keratinocytes.
  8. 8. Triggers  Autoimmunity  Drugs  Infections  Burns  Genetics  Heat, UV rays  Ionizing radiation  Contact dermatitis  Trauma, macerations
  9. 9. Classification Classified as Primary or Secondary  Primary Acantholysis : In these diseases, acantholysis is the primary event leading to formation of intraepidermal cavities and hence the manifestations of the disease.  Secondary Acantholysis : Acantholysis is secondary to alteration / damage to keratinocytes by various factors. So, keratinocytes are injured first followed by subsequent disintegration of desmosomes.
  10. 10. Causes of primary acantholysis  Pemphigus vulgaris and its variants  Pemphigus foliaceous and its variants  Hailey - Hailey disease  Darier’s disease  Transient acantholytic dermatosis  Bullous impetigo  Staphylococcal scalded skin syndrome
  11. 11. Causes of secondary acantholysis  Herpes simplex virus infections  Herpes zoster  Condyloma acuminatum  BT leprosy  Epidermolytic hyperkeratosis  Epidermolysis bullosa  Lethal acantholytic EB  Acantholytic Dowling - Meara type of EB simplex  Mal de Meleda type of PPK
  12. 12.  Galli - Galli disease  Solar keratosis  Acantholytic acanthoma  Adenoid squamous cell carcinoma  Basal cell carcinoma  Keratoacanthoma  Dermatofibroma  Elastolytic granuloma  Leukocytoclastic vasculitis  Psoriasis
  13. 13. Acantholysis in Pemphigus  The conventional concept is that PV IgG binds to Dsgs resulting in stearic hindrance, interference with desmosomal cadherin trans-interactions and loss of intercellular adhesion.  Plasminogen activation in lesional epidermis is also believed to contribute to acantholysis.  The binding of antibodies to peripheral myelin protein (PERP) and/or cellular acetylcholine receptor (AChR), leads to dissociation of adhesion molecules and also initiates apoptosis.
  14. 14.  Subsequently, tonofilaments collapse and keratinocytes shrink with sloughing of desmosomes, which elicits an autoimmune response.  Finally, anti-Dsg antibodies bind to their targets precluding formation of new intercellular junctions.  The linking of the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis has been termed apoptolysis.
  15. 15. Acantholysis in Hailey - Hailey disease  Acantholysis results from a genetically determined defect, resulting in desmosomal instability, in combination with exogenous factors like bacterial toxins, trauma, or maceration.  A mutation in ATP2C1 gene disturbs keratinocyte differentiation, proliferation and adhesion, which in combination with other triggers, results in the separation of keratin filaments from desmosomal plaques.  A similar phenomenon plays a role in Darier's disease.
  16. 16. Biochemical Acantholysis  The term describes a special type of primary acantholysis brought about without antibody mediation.  Drugs and food items of the thiol or phenol groups can trigger pemphigus through this mechanism.  The proposed mechanisms for thiol-induced acantholysis include direct biochemical effect by formation of thiol-cysteine bonds disturbing cell adhesion.
  17. 17. Sites of acantholysis  The site of acantholysis in a given condition helps in the diagnosis, especially in blistering disorders.  In PV, the clefting occurs in the suprabasal epidermis due to involvement of Dsg3, predominantly expressed in the lower epidermis.  In PF and pemphigus erythematosus, acantholysis is usually confined to the sub-corneal layer due to involvement of Dsg1.  In HHD, although acantholysis is often focal or incomplete, it tends to affect the entire epidermal thickness.
  18. 18. Clinical signs associated with acantholysis Nikolsky’s sign  The sign is elicited by applying tangential pressure with a finger or thumb to the affected skin, peri-lesional skin, or normal skin in patients with suspected pemphigus.  It is termed positive if there is extension of the blister and/or removal of epidermis in the rubbed area.
  19. 19.  (a) Eliciting Nikolsky's sign on perilesional skin. Note the tangential pressure, (b) Eliciting Nikolsky's sign, peeling of skin revealing moist erosion
  20. 20.  The underlying pathophysiology of this sign is that acantholysis occurs in affected areas as well as in areas with intact, normal-appearing skin.  In "Nikolsky's phenomenon," the superficial epidermis is felt to move over the deeper layers, and instead of immediate erosion formation as in Nikolsky's sign, a blister develops after sometime.
  21. 21. Bulla spread sign (Asboe-Hansen sign)  It is the enlargement of an intact blister by the application of mechanical pressure on its roof .  If one carefully presses upon the blister, it enlarges towards its periphery due to the mechanical pressure of the blister fluid.  In PV, the blister extension has a sharp angle, whereas in BP, the advanced border is rounded.
  22. 22. Eliciting Bulla spread sign. Note the vertical pressure.
  23. 23. Tzanck Smear  Acantholytic cells are easily demonstrated using a side-lab cytologic smear.  The base of an unroofed blister is gently scraped; the material obtained is gently smeared onto a clean glass slide, allowed to air dry and stained with Giemsa.  A typical acantholytic cell of PV as seen in a Tzanck smear is called a “Tzanck cell”.
  24. 24.  It is a rounded keratinocyte with  hypertrophic or dysmorphic nucleus,  hazy or absent nucleoli,  increased nuclear to cytoplasmic ratio due to the loss of normal intercellular cohesion  abundant eosinophilic to basophilic cytoplasm.  The staining is more intensely basophilic near the cell membrane ('mourning edge') because of cytoplasmic condensation at the periphery, resulting in a perinuclear halo.
  25. 25. Tzanck smear of pemphigus vulgaris showing abundant acantholytic cells. (Giemsa stain, 10× )
  26. 26. High power view of Tzanck cells in pemphigus vulgaris. Note the perinuclear halo visible in the marked cell. (Giemsa stain, 40× )
  27. 27. THANK YOU
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