Examining the Capabilities & Benefits of UHPLC Method Scouting
 

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Ultra high performance liquid chromatography (UHPLC) has become popular due to the interest in improving productivity and lessening the burden on the environment. Method development systems utilizing ...

Ultra high performance liquid chromatography (UHPLC) has become popular due to the interest in improving productivity and lessening the burden on the environment. Method development systems utilizing UHPLC are also becoming an area of interest. This presentation examines the capabilities & benefits of this technology. For more information, visit www.ssi.shimadzu.com and follow us on Twitter @shimadzussi.

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Examining the Capabilities & Benefits of UHPLC Method Scouting Presentation Transcript

  • 1. 1 / 13 Examining the Capabilities and Benefits of UHPLC Method Development Scouting William Hedgepeth, Kenichiro Tanaka Shimadzu Scientific Instruments, Columbia, MD., USA
  • 2. 2 / 132 / 13 Introduction The optimization of analysis conditions in high performance liquid chromatography (HPLC) requires considerable time and effort. Particularly with the analysis of impurities and natural substances, methods tend to be constructed via trial and error. A search for parameters, centered on columns and mobile phases, is required. A variety of method development systems have been devised with the objective of simplifying this process. Nowadays, ultra high performance liquid chromatography (UHPLC) has become popular, due to the interest in improving productivity and lessening the burden on the environment. Method development systems utilizing UHPLC are becoming an area of interest.
  • 3. 3 / 133 / 13 System Requirements Scouting that can be performed quickly and easily by anyone Easy configuration of the mobile phases and columns required for scouting Easy configuration of the equilibration conditions for mobile phase and column switching Quick creation of schedules for scouting Desire to see the scouting conditions reflected in the data sampled
  • 4. 4 / 134 / 13 Interested in: •Investigating conditions using a variety of samples (STD, actual samples, etc.) •Easily configuring gradient and isocratic conditions •Reducing preparation time and eliminating configuration errors •Assessing, in advance, the time required for scouting, as well as the mobile phase quantities and sample quantities •Batch management of columns and mobile phases using a database •Real-time, onscreen confirmation of system status during scouting •Reducing the data analysis time •Identifying, at a glance, the conditions (columns, mobile phases, and gradient conditions) from the name of the data file •Arranging multiple chromatograms in a single window for comparative investigations System Requirements
  • 5. 5 / 135 / 13 SPD-M20A PDA SIL-30AC LC-30AD LC-30AD FCV-34AH MR180 µL Manifold Column CTO-20AC System Configuration Figure 1: Nexera Method Scouting System
  • 6. 6 / 136 / 13Figure 2: Nexera Method Scouting Interface System Configuration Graphical data is provided to support reliable analysis.
  • 7. 7 / 137 / 13 The method scouting system can: •Search conditions based on a maximum of 6 columns and up to 16 mobile phases. •Be used with basically all current UHPLC columns (100 MPa valve pressure resistance). •Easily configure scouting conditions thanks to special software. •Automatically control everything from system checks to scouting and shutdown. •Quickly evaluate scouting results via data browser or CLASS-Agent/Agent Report. System Capabilities
  • 8. 8 / 138 / 13 Benefits of Method Scouting The clear benefit of a method scouting system is time savings derived from the automated process. Columns and mobile phases are placed on the system and set conditions are run without human intervention - over lunch, overnight, or over the weekend - by automated mobile phase and column switching. This allows the method development process to be completed much sooner and frees the analyst’s time for other tasks. A significant amount of time is saved by not needing to manually enter methods and individual injection conditions, made possible by an automated method file and batch creation software.
  • 9. 9 / 139 / 13 Benefits of Nexera Method Scouting OvernightColumn 1 Column 2 Column 3 Column 4 Column 5 Column 6 Column 1 Column 2 Column 3 Column 4 Column 5 Column 6 Overnight Since mobile phasesand columnscan be switched automatically,even overnight,data can be collected efficiently. Nexera Method Scouting System Conventional UHPLC System Day 1 Day 2 Day 3 Day 1 Day 2 Day 4 Analysis All conditionscan be completed and the resultsviewed after 4 days. Overnight Analysis Figure 3: Time Savings with Automated System
  • 10. 10 / 1310 / 13 Automated Method Scouting of Antibiotics Investigation of batch analysis conditions for 13 cephem antibiotic compounds using Nexera Method Scouting and Method Scouting Solution: Structural Formulas for 13 Cephem Antibiotic Compounds
  • 11. 11 / 1311 / 13 Automated Method Scouting of Antibiotics Step 1: Mobile phase and column scouting Purpose: To search for the optimal mobile phase and column combination with universal gradient conditions
  • 12. 12 / 1312 / 13 Automated Method Scouting of Antibiotics Batch analysis conditions for 13 cephem antibiotic compounds are automatically established with Nexera Method Scouting and Method Scouting Solution.
  • 13. 13 / 1313 / 13 Summary 1. Automated UHPLC method scouting is an underutilized technology that greatly reduces method development times. 1. Automated scouting can free an analyst’s time to perform other tasks and increase lab productivity.
  • 14. 14 / 13 Thank you for viewing this presentation. Should you have any questions or require additional information about our research, products or services, please visit our support page: www.ssi.shimadzu.com/support/ @shimadzussiFollow us on Twitter Need More Info?