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GROUP 8           By:Delgado, Sharmaine Kay  Gloria, Sherina Ann   Lagos, Riza Jane  Pillora, Gin Anilou Villaflor, Mary Q...
•   Mass Spectrometry•   Mass Spectrometer•   Principles•   Major Parts•   How it works•   Uses•   Types of Spectrometer
• An analytical technique that  measures the mass-to-charge  (m/z) ratio of charged particles.• A technique of separating ...
• A mass spectrometer is an  analytical tool used to determine  the elemental composition of an  unknown substance. It uti...
• A Mass Spectrometer  produces ions from the  substance under  investigation, separates them  according to their mass-to-...
• Different elements can be  uniquely identified by their mass.
• Different compounds can also be uniquely        identified by their mass.      Butorphanol            L-dopa         Eth...
• The heavier the ion, the lesser  the deflection.• The lighter the ion, the greater  the deflection.
• Mass spectrometers consist of four basic  parts;• a handling system to introduce the unknown  sample into the equipment;...
The sample to be analyzed entersthe instrument through theinlet, usually as a gas, although asolid can be analyzed if it i...
In the ionization chamber, the sample isionized and fragmented. This can beaccomplished in many ways—electronbombardment, ...
3. The Mass Analyser       Here, the particles are separated into   groups by mass, and then the detector   measures the m...
4. The Detector       Finally, a readout device—usually a   computer—records the data.
• The Sample is vaporized into gas for  ionization,• The atom is ionised by knocking one or more  electrons off to give a ...
The ions are accelerated so that they all have the same kinetic energy.
• The ions are then deflected by a magnetic  field according to their masses. The lighter  they are, the more they are def...
The beam of ions passing through the machine is detected electrically.
1. GC/MS (Gas Chromatography-Mass           Spectrometry)        • Is a method that combines          the features of Gas-...
2. AMS (Accelerator Mass     Spectrometry)     • a ‘’tandem accelerator’’       is used to accelerate the       ions at se...
3. ICP-MS (Inductively Coupled Plasma-Mass Spectrometry)• involves the formation of gas  containing electrons, ions and  n...
4. IRMS (Isotope Ratio Mass      Spectrometry) •      It is used to measure     mixture of stable isotopes. It     has two...
5. Tandem MS (Tandem Mass       Spectrometer)  • is a spectrometer used to    separate ions based on a    sample’s ‘’elect...
6. TIMS (Thermal Ionization-     Mass Spectrometry) • is a mass spectrometer that   can make exact   measurements isotope ...
7. SSMS (Spark Source Mass      Spectrometry)• can ionize the analytes in solid  samples using electric current  with two ...
8. (LC/MS or LC-MS) Liquid        chromatography –mass             spectrometry•       It is used to separate    compounds...
9. IMS/MS or IMMS (Ionmobility Spectrometry)• Is a technique where ions are  first separated by drift time  through some n...
• Fast• Differentiates Isotopes• Can be combined with GC  and LC to run mixtures
• Doesn’t directly gives  structural information.• Need pure compounds• Difficult with non-volatile  compounds
45
 Automated analyzers process large volume  of tests with great precision and speed. It permits the operator to focus on ...
47
 Continuous Flow Analyzers Discrete Analyzers                              48
Pumped through a system of continuoustubing. Samples are introduced in asequential manner, following each otherthrough the...
An essential principle of the system is the           introduction of air bubbles.Function of Air Bubbles: The air bubble...
Function of Air Bubbles: The air bubbles also assist mixing by  creating turbulent flow and provide  operators with a qui...
 In Continuous Flow Analysis a  continuous stream of material is divided  by air bubbles into discrete segments in  which...
 The tubing passes the samples from one  apparatus to the other with each apparatus  performing different functions, such...
 Continuous flow is used in some  spectrophotometric instruments in which  the chemical reaction occurs in one reaction  ...
55
 Segmented Stream System     -The reaction stream is segmented with  bubbles of air or nitrogen to reduce inter-sample  d...
It includes a peristaltic pump that continuouslyaspirates sample and reagent, a variable number oftubes constituting a ma...
 At detector air bubbles are removed and each  sample is separated by washing solution, thus a  square shaped detector re...
59
 FIA is based on the injection of a liquid  sample into a moving continuous non  segmented carrier stream of a suitable  ...
 Mixing with reagent in the flowing stream  mainly occurs by diffusion-controlled process  and a chemical reaction occurs...
62
 Discrete analysis is the separation of each  sample and accompanying reagents in a separate  container. Discrete analyz...
 They are the most popular and  versatile analyzers and have almost  completely replaced continuous-flow  and centrifugal...
 Sample reactions are kept discrete through the  use of separate reaction cuvettes, cells, slides, or  wells that are dis...
 Samples are applied to slides that are  automatically dispensed from test- specific  cartridges. Sample application is p...
 The slides incubate in heated air chambers and  the color that develops is measured by  reflectance photometry from the ...
68
Designs of Analyzer Pathway Batch Testing- Samples are processed in concert  as a group or “batch” in the same analytical...
Designs of Analyzer                Pathway Parallel Testing- samples undergo a series of  analytical processes, usually f...
   Patient Identification   Sampling   Sample and Specimen Transport   Dilution   Mixing   Incubation   Reaction Ve...
 Patient identification was accomplished by  transcribing patient information onto sample  cups and print outs of test re...
 Bar code labeling systems are now  employed. The bar code was read and  would match patient data with test  results. The...
74
 Accomplished by syringe pipette or aspirating  probe. The specimens are transferred to  sample cup, and the sample picku...
Sampling-Dispensing Automatic Pipets                      76
A peristaltic pump is a type of positivedisplacement pump used for pumping avariety of fluids.                            ...
Works by squeezing the tube with rollers/shoes. Itcan run dry, self-prime and handle viscous orabrasive liquids, plus, as ...
As the rollers and wiper move, a part of thetube is pressed, causing the fluid to be pumpedonward. A restitution fluid can...
80
A piston pump (reciprocating pumps) is atype of positive displacement pump where thehigh-pressure seal reciprocates with t...
A piston pump uses the reciprocating motion ofa piston rod to move fluid along an axis through acylinder-shaped chamber. A...
83
• Reciprocating pumps will deliver fluid at  high pressure (High Delivery Head).• They are Self-priming - No need to fill ...
 Discrete analyzers employ a variety of  syringe pipettes to aspirate and dispense  sample and reagents. An important  co...
In continuous flow analyzers, specimentransport is accomplished using the peristalticpump. Air bubbles separate aliquots o...
 In the Dupont aca, the sample reagent pack is  transported throughout the analyzer with a chain-  driven pulley system....
 The Kodak Ektachem analyzers  meters the sample aliquot, by use  of a disposable sample tip  secured by an apparatus cal...
 Sample and reagent dilutions are usually  accomplished with the syringe pipettes and  pumps. The pumps must be designed ...
In an automated system such ascontinuous analyzer mixing of asample and reagents is accomplishedusing a glass coil inserte...
 In the Beckam ASTRA systems, a  magnetically driven Teflon stirring bar  located in the bottom of the reaction  chamber ...
 Reaction mixtures that require incubation must  be conducted at constant temperatures without  significant fluctuations....
 In CFA systems the tubing serves as reaction  vessel. In DA, any of the following maybe used:a.) The DuPont aca uses a ...
c.) Hitachi series and Baxters Paramax 720 ZXuse plastic cuvettes.d.) Eastman Kodak Ektachem uses a multilayerthin film sl...
 Light-emitting diodes offer direct readout of  absorbance and replace the earlier recorders with an  ink pen to trace th...
 Calculations, calibration curves, and  quality control are performed by the  computers, thus reducing errors and  provid...
 Most automated chemistry analyzers use  photometric methods of analysis such as  spectrophotometry, fluorometry, nephelo...
 Automated systems based on colorimetry use  narrow-band interference filters for the isolation of  specific wavelengths....
• Albumin              •   Creatinine• Alkaline phosphatase •   Glucose• Aspartate            •   Inorganic phosphorus  tr...
 Increase the number tests performed by one  medical technologist in a given period. Minimize the variation in results f...
 Instruments can use very small amounts of  samples and reagents. Reduction in the variability of results and  errors of...
 Faster analyses up to 120 samples per hour Automatic data recording and preparation Being a closed system, automation ...
 Time-consuming sample preparation steps such  as distillations, digestions, and matrix removal or  enhancement performed...
• is defined as medical testing  at or near the site  of patient care outside of the  conventional laboratory. .• brings t...
• point-of-care test systems are easy-to-use  membrane-based test strips, often enclosed by a  plastic test cassette.• The...
• Non-automated Methods- may be done by  manual rapid-testing methods using a Dipsticks  or Immunostrips.• Instrument-Base...
• Most of the instruments utilized for POCT use  whole blood for analysis and disposable reagent  unit-dose devices.• The ...
• used to measure blood  gas, pH, electrolytes, and some metabolites in  whole blood specimens.• They are also used to det...
• It have extensive test menus and  provide a rapid laboratory results to  expedite a patient’s diagnosis and  treatment.•...
• BLOOD GLUCOSE TESTING• Blood glucose levels are measured by a meter  and use a capillary blood directly from finger  sti...
Drugs of Abuse Testing• Drug of abuse testing are frequently  ordered on patients who exhibit symptoms  of intoxication or...
• Taking the sample from the wrong patient• Taking the wrong type of sample• Failure to follow procedure• Incorrect result...
• Rapid test results essential for decision-making• A system that generates a printout of the  results• Requires small sam...
• Potentially different reference ranges• Costly to operate• Minimal training of personnel to  operate the instruments• Ma...
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
Group 8 mass spec automated analyser and poct the complete version
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Transcript of "Group 8 mass spec automated analyser and poct the complete version"

  1. 1. GROUP 8 By:Delgado, Sharmaine Kay Gloria, Sherina Ann Lagos, Riza Jane Pillora, Gin Anilou Villaflor, Mary Queen 1
  2. 2. • Mass Spectrometry• Mass Spectrometer• Principles• Major Parts• How it works• Uses• Types of Spectrometer
  3. 3. • An analytical technique that measures the mass-to-charge (m/z) ratio of charged particles.• A technique of separating and identifying molecules based on its mass.
  4. 4. • A mass spectrometer is an analytical tool used to determine the elemental composition of an unknown substance. It utilizes the charged particles of molecules to separate them.
  5. 5. • A Mass Spectrometer produces ions from the substance under investigation, separates them according to their mass-to- charged ratio (m/z) and records the relative abundance of each present.
  6. 6. • Different elements can be uniquely identified by their mass.
  7. 7. • Different compounds can also be uniquely identified by their mass. Butorphanol L-dopa Ethanol N -CH2- COOH OH HO -CH2CH-NH2 CH3CH2OH HOHOMW = 327.1 MW = 197.2 MW = 46.1
  8. 8. • The heavier the ion, the lesser the deflection.• The lighter the ion, the greater the deflection.
  9. 9. • Mass spectrometers consist of four basic parts;• a handling system to introduce the unknown sample into the equipment;• an ion source, in which a beam of particles characteristic of the sample is produced;• an analyzer that separates the particles according to mass; and• a detector, in which the separated ion components are collected and characterized.
  10. 10. The sample to be analyzed entersthe instrument through theinlet, usually as a gas, although asolid can be analyzed if it issufficiently volatile to give off atleast some gaseous molecules.
  11. 11. In the ionization chamber, the sample isionized and fragmented. This can beaccomplished in many ways—electronbombardment, chemicalionization, laser ionization, electric fieldionization—and the choice is usually based onhow much the analyst wants the molecule tofragment.
  12. 12. 3. The Mass Analyser Here, the particles are separated into groups by mass, and then the detector measures the mass-to-charge ratio for each group of fragments by electromagnetic fields.
  13. 13. 4. The Detector Finally, a readout device—usually a computer—records the data.
  14. 14. • The Sample is vaporized into gas for ionization,• The atom is ionised by knocking one or more electrons off to give a positive ion.• The Ion source is maintained in a high vacuum environment to enhance collision efficiency and ion formation.
  15. 15. The ions are accelerated so that they all have the same kinetic energy.
  16. 16. • The ions are then deflected by a magnetic field according to their masses. The lighter they are, the more they are deflected.• The amount of deflection also depends on the number of positive charges on the ion - in other words, on how many electrons were knocked off in the first stage. The more the ion is charged, the more it gets deflected.
  17. 17. The beam of ions passing through the machine is detected electrically.
  18. 18. 1. GC/MS (Gas Chromatography-Mass Spectrometry) • Is a method that combines the features of Gas-Liquid Chromatography and Mass Spectrometry to identify the different substances within a sample.
  19. 19. 2. AMS (Accelerator Mass Spectrometry) • a ‘’tandem accelerator’’ is used to accelerate the ions at several million volts.
  20. 20. 3. ICP-MS (Inductively Coupled Plasma-Mass Spectrometry)• involves the formation of gas containing electrons, ions and neutral particles from Argon gas. The sample is atomized and ionized by this gas. In a high vacuum mass analyzer, these ionized atoms from gas are passed through cones (apertures).
  21. 21. 4. IRMS (Isotope Ratio Mass Spectrometry) • It is used to measure mixture of stable isotopes. It has two inlets that help in repetitive measurements with continuous supply of sample gas.
  22. 22. 5. Tandem MS (Tandem Mass Spectrometer) • is a spectrometer used to separate ions based on a sample’s ‘’electronic’’ mass using two or more quadruple’s
  23. 23. 6. TIMS (Thermal Ionization- Mass Spectrometry) • is a mass spectrometer that can make exact measurements isotope ratios of thermally ionisable elements. This ionization can be done by passing them through metal ribbons under vacuum.
  24. 24. 7. SSMS (Spark Source Mass Spectrometry)• can ionize the analytes in solid samples using electric current with two electrodes. It works as one electrode if the sample is metal or can be placed in a cup-shaped electrode by mixing with graph detected isotopes from the sample.
  25. 25. 8. (LC/MS or LC-MS) Liquid chromatography –mass spectrometry• It is used to separate compounds chromatographically before they are introduced to the ion source and mass spectrometer. LC-MS is a powerful technique used for many applications which has a very high sensitivity and selectivity.
  26. 26. 9. IMS/MS or IMMS (Ionmobility Spectrometry)• Is a technique where ions are first separated by drift time through some neutral gas under an applied electrical potential gradient being introduced into mass spectrometer.
  27. 27. • Fast• Differentiates Isotopes• Can be combined with GC and LC to run mixtures
  28. 28. • Doesn’t directly gives structural information.• Need pure compounds• Difficult with non-volatile compounds
  29. 29. 45
  30. 30.  Automated analyzers process large volume of tests with great precision and speed. It permits the operator to focus on tasks that cannot be readily automated and increased both efficiency and capacity. 46
  31. 31. 47
  32. 32.  Continuous Flow Analyzers Discrete Analyzers 48
  33. 33. Pumped through a system of continuoustubing. Samples are introduced in asequential manner, following each otherthrough the same network. This analyzer is capable of analyzingone analyte at a time. 49
  34. 34. An essential principle of the system is the introduction of air bubbles.Function of Air Bubbles: The air bubbles segment each sample into discrete packets and act as a barrier between packets to prevent cross contamination as they travel down the length of the tubing. 50
  35. 35. Function of Air Bubbles: The air bubbles also assist mixing by creating turbulent flow and provide operators with a quick and easy check of the flow characteristics of the liquid. 51
  36. 36.  In Continuous Flow Analysis a continuous stream of material is divided by air bubbles into discrete segments in which chemical reactions occur. The continuous stream of liquid samples and reagents are combined and transported in tubing and mixing coils. 52
  37. 37.  The tubing passes the samples from one apparatus to the other with each apparatus performing different functions, such as distillation, dialysis, extraction, ion exchange, heating, incubation, and subsequent recording of a signal. 53
  38. 38.  Continuous flow is used in some spectrophotometric instruments in which the chemical reaction occurs in one reaction channel and then is rinsed out and reused for the next sample, which may be an entirely different chemical reaction. 54
  39. 39. 55
  40. 40.  Segmented Stream System -The reaction stream is segmented with bubbles of air or nitrogen to reduce inter-sample dispersion. Flow Injection Analysis - It is low pressure and without separation. The injected sample mixes and reacts with the flowing stream. 56
  41. 41. It includes a peristaltic pump that continuouslyaspirates sample and reagent, a variable number oftubes constituting a manifold to circulate liquidand a detector system. Aspirated sample are segmented by injecting airbubbles that should be remove before they canreach to the detector. 57
  42. 42.  At detector air bubbles are removed and each sample is separated by washing solution, thus a square shaped detector response is obtained, the height of rectangle is directly proportional to concentration of analyte. 58
  43. 43. 59
  44. 44.  FIA is based on the injection of a liquid sample into a moving continuous non segmented carrier stream of a suitable liquid. The injected sample forms a zone which is then transported towards a detector. 60
  45. 45.  Mixing with reagent in the flowing stream mainly occurs by diffusion-controlled process and a chemical reaction occurs. Detectors continuously record the physical parameter as it changes as a result of passage of sample material through flow cell. 61
  46. 46. 62
  47. 47.  Discrete analysis is the separation of each sample and accompanying reagents in a separate container. Discrete analyzers have the capability of running multiple tests on one sample at a time or multiple samples one test at a time. 63
  48. 48.  They are the most popular and versatile analyzers and have almost completely replaced continuous-flow and centrifugal analyzers. 64
  49. 49.  Sample reactions are kept discrete through the use of separate reaction cuvettes, cells, slides, or wells that are disposed of following chemical analysis. This keeps sample and reaction carryover to a minimum but increases the cost per test due to disposable products 65
  50. 50.  Samples are applied to slides that are automatically dispensed from test- specific cartridges. Sample application is performed by means of individual, disposable tips, thereby eliminating the carryover problem. The sample itself provides the liquid necessary to hydrate the reagent layers of the slide. 66
  51. 51.  The slides incubate in heated air chambers and the color that develops is measured by reflectance photometry from the bottom side of the slide. Results for each sample are collated and printed in a report form that could be suitable for use as the final chartable report. 67
  52. 52. 68
  53. 53. Designs of Analyzer Pathway Batch Testing- Samples are processed in concert as a group or “batch” in the same analytical analysis. Sequential Testing – samples are processed sequentially rather than in a batch. 69
  54. 54. Designs of Analyzer Pathway Parallel Testing- samples undergo a series of analytical processes, usually for one analysis at a time, often used with batch analysis. Random access testing- a system where any specimen can be analyze in any sequence with regard to the initial order of the specimens. 70
  55. 55.  Patient Identification Sampling Sample and Specimen Transport Dilution Mixing Incubation Reaction Vessels Analysis of Measurement 71
  56. 56.  Patient identification was accomplished by transcribing patient information onto sample cups and print outs of test results. With the arrival of computers, the operator could input patient information to the laboratory computer. 72
  57. 57.  Bar code labeling systems are now employed. The bar code was read and would match patient data with test results. The use of bar code labels has served to reduce errors in matching test results with the proper patient. 73
  58. 58. 74
  59. 59.  Accomplished by syringe pipette or aspirating probe. The specimens are transferred to sample cup, and the sample pickup device aspirates the specimen. In CFA, the aspirating probe is dipped into the sample cup and the specimen is drawn up using a peristaltic pump. 75
  60. 60. Sampling-Dispensing Automatic Pipets 76
  61. 61. A peristaltic pump is a type of positivedisplacement pump used for pumping avariety of fluids. 77
  62. 62. Works by squeezing the tube with rollers/shoes. Itcan run dry, self-prime and handle viscous orabrasive liquids, plus, as the tube is one completeunit, there are no seals thus making the pump leakfree and hygienic. Excellent for dosing applications.Although this principle applies to all peristalticpumps the difference is in the head and the drives. 78
  63. 63. As the rollers and wiper move, a part of thetube is pressed, causing the fluid to be pumpedonward. A restitution fluid can be sent into thepump as the rotors and rollers moved back theprocess is called Peristalsis„. It forms the basicfunction within a Peristaltic Pump. 79
  64. 64. 80
  65. 65. A piston pump (reciprocating pumps) is atype of positive displacement pump where thehigh-pressure seal reciprocates with the piston.Piston pumps can be used to move liquids orcompress gases. Powered by an electricmotor, steam or a turbine, hydraulic drivemechanism. 81
  66. 66. A piston pump uses the reciprocating motion ofa piston rod to move fluid along an axis through acylinder-shaped chamber. As the piston movesthrough the cylinder, pressure builds up and forcesthe fluid through the pump. The fluid flowingthrough the pump pulsates due to the movement ofthe piston through the cylinder. 82
  67. 67. 83
  68. 68. • Reciprocating pumps will deliver fluid at high pressure (High Delivery Head).• They are Self-priming - No need to fill the cylinders before starting. 84
  69. 69.  Discrete analyzers employ a variety of syringe pipettes to aspirate and dispense sample and reagents. An important consideration for any sampling device is specimen carry-over and therefore it should be designed to reduce this problem. 85
  70. 70. In continuous flow analyzers, specimentransport is accomplished using the peristalticpump. Air bubbles separate aliquots of the samesample and isolate one specimen from another. 86
  71. 71.  In the Dupont aca, the sample reagent pack is transported throughout the analyzer with a chain- driven pulley system. Some analyzers used a motorized carousel, for example, the Olympus Demand, to move the reaction vessel in a circular path within the instrument. 87
  72. 72.  The Kodak Ektachem analyzers meters the sample aliquot, by use of a disposable sample tip secured by an apparatus called proboscis, onto a slide for transport to incubation chambers and detectors. 88
  73. 73.  Sample and reagent dilutions are usually accomplished with the syringe pipettes and pumps. The pumps must be designed to aspirate and deliver precise volumes of fluid. The dilution volumes maybe adjusted by use of a cam or programmed via a microprocessor as seen in many discrete analyzers. 89
  74. 74. In an automated system such ascontinuous analyzer mixing of asample and reagents is accomplishedusing a glass coil inserted into the flowpath. As the sample mixture passesthrough the coil, it is inverted andmixed via gravity. 90
  75. 75.  In the Beckam ASTRA systems, a magnetically driven Teflon stirring bar located in the bottom of the reaction chamber is used. The DuPont aca employs a breaker mixer that mechanically vibrates and shakes the pack. 91
  76. 76.  Reaction mixtures that require incubation must be conducted at constant temperatures without significant fluctuations. a.) heating the air around the cuvette b.) heating metal blocks c.) using water baths. 92
  77. 77.  In CFA systems the tubing serves as reaction vessel. In DA, any of the following maybe used:a.) The DuPont aca uses a sealed plastic bag that also serves as the cuvette.b.) The Teflon or plastic rotors in centrifugal analyzers serves as the reaction vessels. 93
  78. 78. c.) Hitachi series and Baxters Paramax 720 ZXuse plastic cuvettes.d.) Eastman Kodak Ektachem uses a multilayerthin film slide. Each slide is impregnated withreagents. Sample cup via a disposable pipette tiponto the slide that also serves as the cuvette forthe reflectance or electrochemical measurement. 94
  79. 79.  Light-emitting diodes offer direct readout of absorbance and replace the earlier recorders with an ink pen to trace the response of the phototube on paper. Computer in the laboratory instrumentation allowed users to display results in a variety of formats and printers provide a hard copy of patient‟s results. 95
  80. 80.  Calculations, calibration curves, and quality control are performed by the computers, thus reducing errors and providing more accurate results than a non-computerized instrument. 96
  81. 81.  Most automated chemistry analyzers use photometric methods of analysis such as spectrophotometry, fluorometry, nephelometry, an d reflectometry. Some analytes, for example sodium and potassium, require the use of electrochemistry for analysis. Instrument manufacturer have designed electrochemical devices based on coulometry, amperometry, and potentiometry to measure these and other analytes. 97
  82. 82.  Automated systems based on colorimetry use narrow-band interference filters for the isolation of specific wavelengths. The filters are contained in a circular disk, called a filter wheel, that rotates into the light path. A computer controls the rotation of the filter wheel and multiple wavelengths can be use to analyze a specimen. 98
  83. 83. • Albumin • Creatinine• Alkaline phosphatase • Glucose• Aspartate • Inorganic phosphorus transaminase (AST) • Protein• Blood urea nitrogen, • Uric acid in bloods• Bilirubin • Calcium• Cholesterol 99
  84. 84.  Increase the number tests performed by one medical technologist in a given period. Minimize the variation in results from one medical technologist to another. Automation eliminates the potential errors of manual analyses as a volumetric pipetting steps, calculation of results, and transcription of results. 100
  85. 85.  Instruments can use very small amounts of samples and reagents. Reduction in the variability of results and errors of analysis through the elimination of task that are repetitive and monotonous for most individuals. 101
  86. 86.  Faster analyses up to 120 samples per hour Automatic data recording and preparation Being a closed system, automation reduces contamination Greater accuracy and reproducibility of results as all samples are subject to same processes Smaller sample and reagent volumes, reduces cost 102
  87. 87.  Time-consuming sample preparation steps such as distillations, digestions, and matrix removal or enhancement performed manually before testing by a discrete analyzer. Cannot perform complex chemistries such as on- line gas diffusion, dialysis, distillations, extractions, and digestions 103
  88. 88. • is defined as medical testing at or near the site of patient care outside of the conventional laboratory. .• brings the test conveniently and immediately to the patient and increases the possibilities of the patient receiving the test result in a timely manner.
  89. 89. • point-of-care test systems are easy-to-use membrane-based test strips, often enclosed by a plastic test cassette.• These tests require only a single drop of whole blood, urine or saliva, and they can be performed and interpreted by any general physician within minutes.•• POCT are accomplished through the use of transportable, portable, and handheld instruments and test kits.
  90. 90. • Non-automated Methods- may be done by manual rapid-testing methods using a Dipsticks or Immunostrips.• Instrument-Based and Automated Methods- are automated and use a small amount of specimen. This type of automation requires minimal technical support and is easy to use. It includes visual readings, display screen, printer, infrared, wireless radio signals, or modems.
  91. 91. • Most of the instruments utilized for POCT use whole blood for analysis and disposable reagent unit-dose devices.• The most popular POCT instrument is the I-STAT analyzer.
  92. 92. • used to measure blood gas, pH, electrolytes, and some metabolites in whole blood specimens.• They are also used to determine abnormal metabolite and/or electrolyte levels in blood and the patient’s acid-base balance and levels of oxygen/carbon dioxide exchange.
  93. 93. • It have extensive test menus and provide a rapid laboratory results to expedite a patient’s diagnosis and treatment.• There are many compact analyzers available for bedside testing, screening projects, wellness centres, operating rooms and emergency rooms.
  94. 94. • BLOOD GLUCOSE TESTING• Blood glucose levels are measured by a meter and use a capillary blood directly from finger sticks.• The blood glucose test is ordered to measure the amount of glucose in the blood right at the time of sample collection. It is used to monitor glucose levels in persons with diabetes.
  95. 95. Drugs of Abuse Testing• Drug of abuse testing are frequently ordered on patients who exhibit symptoms of intoxication or offer a history of drug ingestion.• Rapid and accurate results are critical to manage patients effectively.
  96. 96. • Taking the sample from the wrong patient• Taking the wrong type of sample• Failure to follow procedure• Incorrect result interpretation
  97. 97. • Rapid test results essential for decision-making• A system that generates a printout of the results• Requires small sample volume• Allows testing in a variety of locations• Potential to improve patient outcome or workflow by having results immediately available• Less traumatic for the patients• Portable devices are used
  98. 98. • Potentially different reference ranges• Costly to operate• Minimal training of personnel to operate the instruments• Management of POCT is challenging• Not all methods are appropriate for diagnosis or monitoring treatment
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